Plant/Animal Whole Genome Sequencing
Features Service
● Praeparatio Library potest esse vexillum vel PC-liberum
● Praesto sunt in 4 suggestis sequencia: Illumina NovaSeq, MGI T7, Nanopore Promethion P48, vel PacBio Revio.
Analysis bioinformatica detectio variantium notarum: SNP, InDel, SV et CNV
Servitium Commoda
●Expertise et Publication Records: experientia congesta in genome sequendo pro super 1000 species consecuta est in casibus supra 1000 editis cum factore cumulativo impulsum super 5000.
●Comprehensive Bioinformatics Analysis: Complectens variationem vocationis et functionis annotationem.
Post-Sales Support:Munus nostrum ultra project complementum cum 3-mensibus post venditionem tempus servitutis extenditur. Hoc tempore consilium praebemus subsequens, adiumentum fermentum, et sessiones Q&A address quaelibet interrogationes ad eventus relatas.
●Comprehensive Annotation: Multiplices databases utimur ut officiatorie gena cum variationibus identificatis annotate et debitam analysi locupletationem perficiamus, perspicientia multiplicium investigationum incepta praebens.
Service Specifications
| Variantes invenire | Sequencing strategy | Commendatur profundum |
| SNP et InDel | Illumina NovaSeq PE150 aut MGI T7 * | 10x |
| SV, CNV (minus accurate) | 30x | |
| SV, CNV (verius) | Nanopore Prom P48 | 20x |
| SNPs, Indels, SV, CNV . | PacBio Revio | 10x |
Sample Requisita
| TEXTUS vel extractum acida nucleica | Illumina/MGI | Nanopore | PacBio
| ||
| Animal Viscera | 0.5-1 g | ≥ 3.5 g
| ≥ 3.5 g
| ||
| Musculus animalis | ≥ 5 g
| ≥ 5 g
| |||
| Mammalia Sanguis | 1.5 mL | ≥ 0.5 mL
| ≥ 5 mL
| ||
| GALLINA / Piscis Sanguinis | ≥ 0.1 mL
| ≥ 0.5 mL
| |||
| Plant- Fresh Folium | 1-2 g* | ≥ 2 g
| ≥ 5 g
| ||
| Cellulae cultae |
| ≥ 1x107
| ≥ 1x108
| ||
| Insectorum mollis TEXTUS / Individual | 0.5-1 g | ≥ 1 g
| ≥ 3 g
| ||
| extractum DNA
| Retrahitur: ≥ 1 ng/ µL Moles: ≥ 30 ng Limited vel nulla turpitudo vel contagione
| Concentratio Moles
OD260/280
OD260/230
Limited vel nulla turpitudo vel contagione
| ≥ 40 ng/ µL 4 µg/fluit cellam/sample
1.7-2.2
≥1.5 | Concentratio Moles
OD260/280
OD260/230
Limited vel nulla turpitudo vel contagione | ≥ 50 ng/ µL 10 μg/profluentia cellula/sample
1.7-2.2
1.8-2.5 |
| PCR-libera Bibliotheca Praeparatio: Concentration≥ 40 ng/ µL Amount≥ 500 ng | |||||
Service Opus O
Sample traditio
DNA extractionem
Library construction
Sequencing
Analysis Data
Data traditio
Sequenti analysi includit:
- Rudis Data Quality Control
- Statistics alignment ut referat genome
- Variae identificatio: SNP, InDel, SV et CNV
- Eget annotationem variantium
Statistics of alignment to reference genome - sequencing profundum distribution
SNP vocans inter multa exempla
InDel identificatio - statistica longitudinis InDel in regione CDS et regione genome-latae
Distributio varia per genome - Circos insidias
Functional annotation of genes with variants identified - Gene Ontology
Chai, Q. et al. (2023) 'A glutathione S‐transferase GhTT19 determinat pigmentationis petalis floris per anthocyanin cumulum in bombacio regulantem', Plant Biotechnology Journal, 21 (2), p. 433. doi: 10.1111/PBI.13965.
Cheng, H. et al. (2023) 'Chromosome-gradus silvestris Heveae brasiliensis genome nova instrumenta praebet ad loci genomico-asistentes et pretiosos ad iuvamen cede elevandum', Plant Biotechnology Journal, 21(5), pp. 1058-1072. doi: 10.1111/PBI.14018.
Li, A. et al. (2021) 'Genome ostreae estuarinae perspectiones in climatis impulsum et ad plasticitatem adaptivam praebet', Communicationes Biologiae 2021 4, 1, 4 (1), pp. doi: 10.1038/s42003-021-02823-6.
Zeng, T. et al. (2022) 'Analysis genome et methylationis in Sinensi pullis indigenis per tempus mutationibus perspectionem in speciebus conservationis praebet', Communicationis Biologiae, 5(1), pp. doi: 10.1038/s42003-022-03907-7.
