PacBio 2+3 Plena Longitudo mRNA SOLUTIO
Features
Study design:
Pooled sampled cum PacBio ad identify transcript isoforms
Exempla singula (replicata et conditiones ut probentur) sequentia suntNGS ad quantitatem transcript expressio
● PacBio sequencing in CCS modum, generans HiFi legit
Sequencing plenae longitudinis transcripts
● Analysis genome respectum non requirit; tamen adhiberi potest
● Analysis bioinformatica non solum expressionem gene et plano isoformi includit, sed etiam analysin lncRNA, fusions gene, poly-adienylation, structura gene
commoda
High Accuracy: HiFi accurate legit >99.9% (Q30), comparandum cum NGS
Alternative Splicing Analysis: sequencing all transcripts dat isoform identificatio et characterisation.
deducto PacBio et NGS Strengths: efficere quantitatem locutionis in plano isoformi, mutationem denudationis quae palliata esse potest cum totam expressionem gene
Extensiva Expertise: cum indagatio peractae super 1100 PacBio incepta plena longitudinis transcripto et dispensando super 2300 exempla, nostra turma experientiae opes ad omne consilium adfert.
Post-Sales Support: munus nostrum ultra project complementum cum 3-mensibus post venditionis tempus extenditur. Hoc tempore consilium praebemus subsequens, adiumentum fermentum, et sessiones Q&A address quaelibet interrogationes ad eventus relatas.
Sample Requisita et Delivery
| Library | Sequencing strategy | Data commendatae | Qualitas Imperium |
| PolyA ditavit variant CCS bibliothecae | PacBio Sequel II PacBio Revio | 20/40 Gb 5/10 M CCS | Q30≥85% |
| Poly A locupletatus | Illumina PE150 | 6-10 Gb | Q30≥85% |
Nucleotides
|
| Conc. | Moles (μg) | Puritas | Integritas |
| Illumina Library | ≥ 10 | ≥ 0.2 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Nullam dapibus vel dapibus vel DNA contagione gel ostensum est. | Pro plantis: RIN≥4.0; Pro animalibus: RIN≥4.5; 5.0≥28S/18S≥1.0; limitata vel non collocantur elevatione |
| PacBio bibliotheca | ≥ 100 | ≥ 1.0 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Nullam dapibus vel dapibus vel DNA contagione gel ostensum est. | Plantae: RIN≥7.5 Animalia: RIN≥8.0 5.0≥28S/18S≥1.0; limitata vel non collocantur elevatione |
Sample Delivery commendatur
Continens: 2 ml tubum centrifugium (foil plumbi non commendatur)
Sample labeling: Group+replicata eg A1, A2, A3; B1, B2, B3.
Shipment:
1. siccum-glacies:Exemplaria in saccis referta necesse est et in siccitate glacie sepeliri.
2. RNAstable tube: RNA specimina in RNA stabilizationis tubo exsiccata possunt (eg RNAstable®) et in cella temperie conscendunt.
Sequenti analysi includit:
Rudis notitia qualis imperium
Alternative Polyadenylation Analysis (APA)
Fusion transcript analysis
Alternative Splicing Analysis
Probatio Universalis Single Exemplar Orthologorum (BUSCO) analysis
Nova analysin transcript: coniectura sequentiarum coding (CDS) et annotationem functionis
lncRNA analysis: vaticinium lncRNA et scuta
MicroSatelite Lepidium sativum (SSR)
Aliter transcripta expressa (DEts) analysis
Differenter expressit Genes (DEGs) analysis
Eget annotationem DEGs et DETs
BUSCO analysis
Alternative Splicing Analysis
Alternative Polyadenylation Analysis (APA)
Differentiter expressit Genes (DEGs) et Transcript (DETs9 anlaysis
Dapibus commercium retiacula DETS et DEGs
Progressiones explorare faciliores per BMKGene's PacBio 2+3 plenam longitudinis mRNA sequencing per collectionem curatam publicationum.
Chao, Q. et al. (2019) 'Motiones evolutionis transcriptome populi Populi, Plant Biotechnology Journal, 17 (1), pp. 206-219. doi: 10.1111/PBI.12958.
Deng, H. et al. (2022) ' Mutationes dynamicae in Ascorbico Acidi contenti per progressionem fructuum et maturationem Actinidiae latifoliae (Ascorbate-Rich Fruit Crop) et Mechanismi associati Moleculares, Acta Scientiarum Molecularium Internationalium, 23(10), p. 5808. doi: 10.3390/IJMS23105808/S1.
Hua, X. et al. (2022) 'Praedictio efficax generum viarum biosyntheticae in polyphyllo bioactivo implicatae in polyphylla Parisiensi', Communicationis Biologiae 2022 5, 1, 5(1), pp. doi: 10.1038/s42003-022-03000-z.
Liu, M. et al. (2023) 'PacBio Iso-Seq deducta et Illumina RNA-Seq Analysis Tuta absoluta (Meyrick) Transcriptome et Cytochrome P450 Genes, Insects, 14(4), p. 363. doi: 10.3390/INSECTS14040363/S1.
Wang, Lijun et al. (2019) 'Aspectio transcriptome complexionis utens PacBio analysi uni-moleculi realis-tempus cum Illumina RNA, ad meliorem intelligentiam acidi ricinoleici biosynthesis in Ricinus communis', BMC Genomics, 20 (1), pp. 1-17. doi: 10.1186/S12864-019-5832-9/FIGURES/7.
