Hi-C fundatur chromatin commercium
Service Features
● sequencing in illumina novaseq cum Pe150.
● ministerium requirit TEXTUS exempla, pro extractum nucleic acida, ut crucem-link cum formaldehyde et conservare DNA-dapibus interactions.
● HI-c experimentum involves restrictione et finem instaurare in lentum extremis cum Biotin, sequitur circularizization ex consequens obtusum fines cum conservando interactiones. Et DNA est igitur extraxerunt cum Streptavidin grana et purificatur ad subsequentem bibliotheca praeparationis.
Service commoda
●Optimal restrictione enzyme consilium: Ut a summo Hi-C efficientiam in diversis speciebus cum usque ad XCIII% valet interaction paria.
●Expertis et publication RecordsBMKGene habet ingens experientia cum> MM Hi-c sequencing projects ex DCCC diversis speciebus et variis patents. Per C publish in casibus cum accumulative impulsum factor de super CM.
●Highly-Peritus Bioinformatics Team:Cum in-Domus Patents et Software Copyrights pro Hi-c Experimenta et data Analysis et auto-developed visualization notitia software.
●Post-Sales Support:Noster commitment extendit ultra project complementum cum III-mensis post-Sale Service tempus. Per hoc tempus, ut offerimus project sequantur, sursum, Troubleshooting auxilium, et Q & sessiones ad oratio quis queries ad praecessi.
●Comprehensive annotation: Non utimur multiple databases ad muneris annotate in genes cum identified variationes et praestare correspondentes locupletantes analysis, providing insights in multiple investigationis projects.
Service Specifications
| Bibliotheca | Sequencing belli | Commendatur data output | Hi-C signa resolutio |
| Hi-c bibliotheca | Illumina pe150 | Chromatin loop: 150X Tad: 50x | Chromatin loop: 10kb Tad: 40kb |
Service Requirments
| Sample Type | Requiritur |
| Animal TEXTUS | ≥2g |
| Totus | ≥2ml |
| Fungi | ≥1g |
| Plant- Young TEXTUS | 1G / Aliquot, 2-4 Aliquats commendatae |
| Cellulas | ≥1x107 |
Includit sequenti analysis:
● rudis data QC;
● Mapping et Hi-c bibliotheca QC: validum interaction binos et commercium labe exponentes (Idibus);
● Genome, wide interaction profiling profiling: cis / trans Analysis et Hi-c commercium tabula;
● analysis of a / b cellula distribution;
● idem Tads et chromatin ansas;
● differentialium analysis in 3D chromatin structuram elementa in samples et correspondentes munus annotationem consociata genes.
Cis et PROCORPATIO Distribution
Heatmap de chromosomal interactiones inter exemplaria
Londini-wide distribution a / b compartments
Genome, wide distribution chromatin ansas
Visualization Tads
Explorarent investigationis progressiones facilitati per BMKGene scriptor Hi-c sequencing Services per a curated collectione publications.
Meng, T. et al. (MMXXI), A comparativum multi-omice analysis agnoscit CA2 sicut novae scopum ad chordomem ';Neuro-oncology, XXIII (X), pp. 1709-1722. Doi: 10.1093 / Neuonc / noab156.
Xu, L. et al. (MMXXI), 3D Disorganization et reorangement of genome providere insights in Pathogenesis de Nafld ab integrated Hi-C, Nanopore et RNA Sequentia,Acta pharmaceutica sinica b, XI (X), pp. 3150-3164. Doi: 10.1016 / j.apsb.201.03.022.
