Plena Longitudo variant Sequencing-Nanopore
Features
Captura poly-A mRNA sequitur synthesin cDNA et praeparatio bibliothecae
Sequencing plenae longitudinis transcripts
Bioinformatic analysis secundum alignment ut referat genome
Analysis bioinformatica non solum expressionem gene et plano isoformi includit, sed etiam analysin lncRNA, fusions gene, poly-adienylationem et structuram gene
Servitium Commoda
●Quantitas locutionis in gradu isoformi: analysin plicare accuratam et accuratam expressionem, mutationem denudationis quae palliata esse potest, cum analysin totius gene.
●Reducuntur Data poscunt:Comparari cum Sequentian (NGS), Nanopore sequencing indicat requisita notitiarum inferiorum, permittens ad aequivalentes gradus quantitatis generum saturationis cum minore notitia.
●Superiore accurate expressio quantitatis; Et ad gene et isoform gradu
●Identification of additional transcriptomic information: polyadenylation, fusion genesis and lcnRNA and their target gene
●Expertise: Manipulus noster opes experientiae ad omne propositum affert, expletis supra 850 Nanopore inceptis plenae longitudinis transcripto et per exempla 8000 discursum.
●Post-Sales Support: munus nostrum ultra project complementum cum 3-mensibus post venditionis tempus extenditur. Hoc tempore consilium praebemus subsequens, adiumentum fermentum, et sessiones Q&A address quaelibet interrogationes ad eventus relatas.
Sample Requisita et Delivery
| Library | Sequencing strategy | Data commendatae | Qualitas Imperium |
| Poly A locupletatus | Illumina PE150 | 6/12 Gb | Mediocris qualitas score: Q10 |
Sample Requisita:
Nucleotides:
| Conc. | Moles (μg) | Puritas | Integritas |
| ≥ 100 | ≥ 1.0 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Nullam dapibus vel dapibus vel DNA contagione gel ostensum est. | Pro plantis: RIN≥7.0; Pro animalibus: RIN≥7.5; 5.0≥28S/18S≥1.0; limitata vel non collocantur elevatione |
● Plantae:
Radix, Caulis vel Petal: 450 mg
Folium vel Semen: 300 mg
Fructus: 1.2 g
Animal:
Cor vel Intestinum: 300 mg
Viscera vel cerebrum: 240 mg
Musculus: 450 mg
Ossa, capillus vel cutis: 1g
Arthropoda:
Insecta: 6g
Crustacea: 300 mg
totum sanguinem: 1 tube
Cellulae: 106 cellulae
Sample Delivery commendatur
Continens: 2 ml tubum centrifugium (foil plumbi non commendatur)
Sample labeling: Group+replicata eg A1, A2, A3; B1, B2, B3.
Shipment:
1. Siccum-glacies: Exemplaria in saccis referta necesse est et in glacie siccitate sepulta.
2. RNAstable tube: RNA specimina in RNA stabilizationis tubo exsiccata possunt (eg RNAstable®) et in cella temperatura exsiccata sunt.
Service Opus O
Nucleotides:
Sample traditio
Library construction
Sequencing
Analysis Data
Post-venditionis officia
Service Opus O
TEXTUS:
Experimentum design
Sample traditio
RNA extractionem
Library construction
Sequencing
Analysis Data
Post-venditionis officia
Rudis MGE
Transumptum identificatio
Alternativum splicing
Expressio quantitatis in gene gradu et gradu isoform
Analysis differentialis expressio
Function annotation and locupletation (DEGs and DETS)
Alterna plicatio analysis
Alternative Polyadenylation Analysis (APA)
lncRNA praedictio
Adnotationes novae genes
Botri DETs
Dapibus Networks in DEGs
Progressiones explorare faciliores per BMKGene's Nanopore plenam longitudinis mRNA sequencing officia per curatam collectionem publicationum.
Gong, B. et al. (2023) 'Activatio epigenetica et transcriptionalis secretoriae kinasis FAM20C sicut oncogene in glioma', Acta geneticorum et genomicorum, 50 (6), pp. doi: 10.1016/J.JGG.2023.01.008.
ipse, Z. et al. (2023) 'Plena longitudo transcriptome sequen- tis lymphocytarum respondentibus IFN-γ, indicat responsionem immunem Th1-skewed in passere (Paralichthys olivaceus)', Piscium & conchylium Immunologiae, 134, p. 108636. doi: 10.1016/J.FSI.2023.108636.
Ma, Y. et al. (2023) 'Comparative analysis of PacBio and ONT RNA sequencing methodos Nemopilema Nomurai veneni identificatio', Genomics, 115(6), p. 110709. doi: 10.1016/J.YGENO.2023.110709.
Yu, D. et al. (2023) Analysis Nano-seq diversam functionem inter exosomes et microvesiculas ab hUMSC derivatas manifestat', Stem Cell Research and Therapy, 14 (1), pp. 1-13. doi: 10.1186/S13287-023-03491-5/TABLES/6.
