Illumina Pre-factum libraries
Features
●Platforms:(VI) et novasequ X plus illumina
●Sequitiones modos:Pe150 et P250
●Qualis imperium de libraries ante sequencing
●Delivery sequencing data QC:Delivery de QC fama et rudis notitia in fastq forma post demultiplexing et filtering q30 legit
Service commoda
●Sequing Services of Services:Et Customer potest eligere ut sequence per Lane, fluxus cellula, aut per moles notitia requiritur (partialis lane sequencing).
●Experientia exstative in illumina sequencing platform:et millia de clausis projects variis speciebus.
●Delivery de Sequentia QC Report:Cum qualis metrics, data accurate et altiore perficientur de sequencing project.
●Mature sequencing processus:cum brevi rursus-circa est.
●Rigorous species imperium: Nos effectum deducendi stricto QC requisitis ad praestigiam partum ex constanter summus qualitas results.
Sample Platforms
| Suggestus | Fluxus | Sequencing modus | Unitas | Aestimari output |
| Novaseq x | 10b (VIII vicis) | Pe150 | Unius lane Lane | 375GB / Lane |
| 25B (VIII vicos) | Pe150 | Unius lane Lane | M GB / lane | |
| Novaseq (VI) | SP Flow Cell (II vicos) | Pe250 | Fluxus Unius lane Lane | 325-400 m legit / lane |
| S4 fluxus cellula (IV vicos) | Pe150 | Fluxus Unius lane Lane | ~ DCCC gb / lane |
Sample Requirements
| Notitia moles (X) | Concentration (QPCR / NM) | Volumen | |
| Partialis lane sequencing
| X ≤ X GB | ≥ I NM | ≥ XXV μl |
| X GB <x ≤ L GB | ≥ II NM | ≥ XXV μl | |
| L GB <X ≤ C GB | ≥ III NM | ≥ XXV μl | |
| X> C GB | ≥ IV NM | ≥ XXV μl | |
| Lane sequencing | Per lane | ≥ 1.5 NM / Library stagnum | ≥ XXV μl / bibliotheca stagnum |
Praeter concentration et totalis moles, apta apicem exemplum etiam requiritur.
Note: Lane Sequitiones ex humilis diversitas libraries requirit Phix Spike-in ut robust basi vocant.
Nos suadeo submittendo pre-pooled libraries ut samples. Si requirere BMKGene ad praestare bibliotheca pooling, placere ad
In bibliotheca requisita pro partialis lane sequencing.
Library Size (Pecco Map)
Pelagus apicem sit in 300-450 BP.
Libraries sit unum principalis apicem, nibh contaminationem et non primario.
Quaeso pervenire ad nos, si vestri exempla non occurrit in incipiens materia requisita.
Opera workflow
Bibliotheca species potestate
Sequencing
Data qualis imperium
Project Delivery
Library QC fama
A fama in qualis est bibliothecam provisum est ante sequencing, censu bibliotheca moles et fragmentation.
Sequencing QC Report
Tabula I. Statistics in sequencing notitia.
| Sample id | Bmkid | Rudis legit | Cruda Data (BP) | Mundus legit (%) | Q20 (%) | Q30 (%) | GC (%) |
| C_01 | Bmk_01 | 22,870,120 | 6,861,036.000 | 96.48 | 99.14 | 94.85 | 36.67 |
| C_02 | Bmk_02 | 14,717.867 | 4,415,360,100 | 96.00 | 98.95 | 93.89 | 37.08 |
Figura I. Qualis distribution per legit in se sample
Figura II. Base contentus distribution
Figura III. Contents in sequencing Distribution of notitia
