BMKMANU S3000_Spatial Transcriptome
BMKMANU S3000 spatii transcriptome technica ratio
Features
- Resolution: 3.5 μm
- macula Diameter: 2.5 μm
- Number de maculis: circiter IV decies centena
- III fieri potest captis area formats: 6.8 mm * 6.8 mm, XI mm * XI mm, aut XV mm * XX mm
- Quisque barcoded bead oneratur cum primers composito ex IV sectiones:
• Poly (DT) cauda quia MRNA priming et CDNA synthesis,
• Unique Momecular identifier (Umi) ad rectam amplificare Bias
• Spatium Barcode
• Binding Sequence of Partiales Read I Dimer
- H & E et fluorescent tinguendo de sectiones
- possibilitate ad usum cell segmentation technology: Integration H & E tingunt, fluorescent tinguendo, et RNA sequencing ad determinare terminos cuiusque cellula et bene assignare gene exprimere se cellula. Processing Downstream Spatium profiling Analysis secundum cellulam bin.
- potest ad consequi multilevel resolutio analysis: flexibilia multi-gradu analysis vndique a 100um ad 3.5 um ad propono diversi TEXTUS features ad optimal resolutio.
S3000 commoda BMKMANU
-IV decies centena captis maculis: Cum enim melius resolutio 3.5 um, ducens in altiorem gene et UMI deprehendatur per cellulam. Hic results in melius cellulis cellulis secundum transcriptional profiles, cum pulcherrima detail quod aequet textuum structuram.
- Sub-Cellularium resolutio:Quisque captis regio continebat> II decies locorum barcoded maculis cum diameter 2.5 μm et spacing of V μm inter macula centers, enabling spatio transcriptome analysis cum sub-cellular resolution (μm).
-Multi gradu resolutio analysis:Flexibile Multi-gradu analysis vndique a C μm ad V μm ad propono diverse TEXTUS features ad optimal resolutio.
-Possibilitate ad usum "tria in uno slide" Cell Arthropoda Technology:Combining Fluorescence Odbreing, H & Eggleing et RNA Sequencing in unum Slide, noster "tres-in-unum" analysis algorithm potestate et idem in cellula terminos subsequent cellulam-fundatur transcripomics.
-Compatible cum multa sequencing platforms: Utrumque NGS et Long-lego sequencing praesto.
-Flexibile consilio 1-8 activae captis regio: In magnitudine captis area est flexibile, esse potest ad usum III formats (6.8 mm * 6.8 mm., XI mm * XI mm * XI mm * XI mm * XI mm * XI mm.
-Unum-subsisto ministerium: Integrates omnes experientia et arte-fundatur gradibus, inter Cryo-sectioning, tinguere, TEXTUS Optimization, loci Barcoding, Library Praeparatio, sequencing, et Bioinformatics.
-Comprehensive Bioinformatics et User-Friendly Visualization Results:Package includes XXIX Analyses et 100 High-qualitas figuras, combined cum usu inhouse developed software ad visualize et customize cellam scissionem et macula clustering.
-Nativus notitia analysis et visualization: Available pro diversis investigationis petitiones
-Technical Team peritissimus: Cum experientia in super CCL TEXTUS types et 100 species inter humanum, mus, mammal, pisces et plantis.
-Verus, tempus updates in integrum project: Cum plena potestate experimentalem profectum.
-Ad libitum iuncturam analysis cum uno-cellula mRNA sequencing
Service Specifications
| Sample Requirements | Bibliotheca | Sequencing belli | Notitia commendatur | Imperium |
| Oct-embedded Cryo Samples (Optimal Diameter: proxime. VI × VI × VI mm³) II cuneos per sample I ad experimentum, I ad tergum | S3000 CDNA Library | Illumina pe150 | 160k PE legit per 100υm (CCL MB) | Rin> VII |
Nam More Details on Sample praeparatio ductu et servitium workflow, placet sentire liberum loqui adBMKGene Peritus
Service opus influunt
In sample praeparatio tempus, an initial mole RNA extractionem iudicii fit ut a summus qualis RNA potest adeptus. In TEXTUS Optimization scaena in sectiones sunt maculata et visualized et permeabilization conditionibus in mRNA dimittere ab TEXTUS sunt optimized. Et optimized protocol est igitur applicari in bibliotheca constructione, sequitur sequencing et notitia analysis.
In completum ministerium workflow involves realis-vicis updates et client confirmationes ad ponere responsive feedback loop, cursus lenis project.
Et data generatae per BMKMANU S3000 est resolvitur usura software "bstmatrix", quae est independenter disposito per BMKNGGEGNE, generating cellulam gradu et multileve resolutio gene expressio matricem. Ex illuc, a vexillum fama generatur quod includit data qualis imperium, interiorem-sample analysis et inter-coetus analysis.
- Data Qualitas Imperium:
- Data output et qualis score distribution
- Gene deprehendatur per macula
- TEXTUS coverage
- Interiorem-Sample Analysis:
- Gene dives
- macula bottering, inter reducta dimensionem analysis
- differentialium Analysis Inter Botters: Lepidium sativum Haging
- Eget Annotation et locupletam de titulum Genes
- Inter-coetus analysis
- Re-combination de maculis ex utroque exempla (eg. Inference et control) et rursus botro
- Lepidium sativum in titulum genes enim inter botrus
- Eget Annotation et locupletam de titulum Genes
- differentialium expressio eiusdem botrus inter coetibus
Praeterea, in BMKGene developed "bstvviewer" est a user-amica instrumentum quod dat user ad visualize in gene expressio et macula brustering ad diversas animus.
BMKGene offert spatia profiling officia ad precise uno-cell resolutio (secundum cellulam bin vel multileve quadrata bin a 100um ad 3.5um).
Spatium profiling notitia ex TEXTUS sectiones in S3000 slide fieri etiam infra.
Case Study I: Mus cerebrum
Analysis de mus cerebrum sectionem cum S3000 exitus in idem ~ XCIV 000 cellulis, cum medianus sequencing of ~ MM genes per cellula. In melius resolutionem 3.5 um resoluta in a valde detailed botrum cellulis secundum transcriptional exemplaria, cum botri cellulis mimicking cerebri diversificantur structuris. Facile observari visualizing distributionem cellulis clusters sicut oligodendrocysticis et microglia cellulis, quae prope solum sita in griseo et albas re respective.
Case Study II: Muris Embryo
Analysis de mus Empryo sectionem cum S3000 exitus in idem ~ MMCC 000 cellulis, cum medianus sequencing de ~ MDC genes per cellulam. Et improved resolution of 3.5 um in a valde detailed botrum cellulis secundum transcriptional exemplaria, cum XII botters in area oculi et XXVIII clusters in area de cerebri.
Interiorem-Sample Analysis cellula clustering:
Venalicium genes idem et spatio distribution:
- higher sub-cellular resolution: Compared with S1000 slide, each capture area of S3000 contained >4 million spatial Barcoded Spots with a diameter of 2.5 µm and a distance of 3.5 µm between spot centers, enabling spatial transcriptome analysis with higher sub-cellular resolution (Square Bin: 3.5 μm).
- Superius captis Efficens: comparari cum S1000 slide, median_umi proventus de XXX% ad LXX%, median_gene auget a XXX% ad LX%
S1000 chip consilium:
S3000 chip ratione
