BMKMANU S1000 Spatial Transcriptome
BMKMANU S1000 Spatial Transcriptome Technical Scheme
Features
● Resolutio: 5 µM
Macula Diameter: 2.5 µM
Number maculae: circiter II Million
● 3 comprehendi possunt formatos areae: 6.8 mm * 6.8 mm, 11 mm * 11 mm vel 15 mm * 20 mm.
● Singula capita barcoded primers onusta ex 4 sectionibus componuntur;
poly(dT) cauda pro mRNA priming et cDNA synthesis
Unique Identifier (UMI) ad corrigendam amplificationem biam
Locus barcode
Binding sequence of partial read 1 sequencing primer
H & E et fluorescentium tinguere sectionum
possibilitas ad usumcellula justo technology: integratio H&E maculans, fluorescentium maculans, et RNA sequens ut fines cuiusque cellulae definiant et singularem cellam gene expressionem recte assignent.
Commoda BMKMANU S1000
●Resolutio sub-cellularia: Singulae areae captae continebant >2 decies centena spatia locorum Barcoded macularum diametro 2.5 µm et spatium 5 µm inter centra macula, ut analysin transcriptoma spatii cum resolutione sub-cellularet (5 µm).
●Multi-level Resolutio Analysis:Flexibile analysis multi-gradum ab 100 μm ad 5 µm ad varias textus lineas componendas in optimali resolutione.
● Possibilitas utendi "Tres in uno lapsu" Cell Segmentation Technologiae:Coniungens fluorescens maculans, H&E maculans, et RNA sequens in unum labium, noster analysis algorithmus "tres-in-unum" efficit ut identificatio limitum cellularum pro posterioribus transcriptomics cellulis substructis.
●Compatible cum Multiplex Sequencing Platforms: Both NGS and long-read sequencinging available.
●Flexibile Design 1-8 Active Captura Area: Magnitudo areae captivitatis flexibilis est, cum 3 formatis possibilibus uti (6.8 mm *6,8 mm., 11 mm * 11 mm et 15 mm * 20 mm).
●One-subsisto Service: omnem experientiam et peritiam substructi gradus integrat, incluso cryo-sectione, tinguendo, texti optimiizationis, spatiis barcoding, bibliothecam praeparationem, sequencem, et bioinformaticas.
●Comprehensiva Bioinformatica et User-amica Visualization proventuum:involucrum includit 29 analyses et figuras 100+ qualitates altae, coniuncta cum usu programmatis inhouse effectae ad visualizandum et mos cellulam scindendam et maculam condensandam.
●nativus Data Analysis et Visualization: Praesto pro diversis petitiones investigationis
●Peritus Technical Team: experientia in supra 250 textuum generibus et 100+ speciebus inter homines, murem, mammalem, pisces et plantas.
●Real-time Updates Totam Project: with full control of experimental progress.
●Libitum Iuncturam Analysis cum Single-cell mRNA Sequencing
Service Specifications
|
Sample Requisita
| Library |
Sequencing strategy
| Data commendatae | Qualitas Imperium |
| OCT embedded cryo exempla, 3 caudices per sample | S1000 cDNA library | Illumina PE150 (alia suggesta praesto) | 100K PE legit per 100 uM ( 60-150 Gb ) | RIN>7 |
Plura de praeparatione exempli in regimine et servitio workflow, placet liberum loqui ad aBMKGENE expert
Service Opus O
In praeparatione exempli, mole RNA extractio initialis iudicium conficitur ut RNA summus qualitas obtineatur. In scaena optimae textus sectiones maculatae et subjiciuntur et condiciones permeabiliizationis pro variantibus emissione textus optimized sunt. Protocollum optimized tum applicatur in constructione bibliothecae, sequendo sequendo et analysi.
Totum ministerium workflow involvit real-time updates et clientelam confirmationes ad servandum responsativum feedback ansam, ut lenis project exsecutionem procurans.
Notitia a BMKMANU S1000 generata inspicitur usura programmatis "BSTMatrix", quae independenter a BMKGENE designata est, gene expressionem Matrix generans. Inde signum normae gignitur quae includit notas qualitatis imperium, analysin interna sample analysi et inter coetus analysi.
Data Quality Control:
Data output et qualis score distribution
Gene deprehendatur per macula
TEXTUS coverage
● Analysis interioris specimen:
Gene divitiae
Macula pampineis, inclusis dimensionis analysin reducta
Analysis differentialis expressio inter ligaturas: idem titulus genes
Functional annotation and locupletation genes
Analysis inter coetus:
Re- deductio macularum ab utroque exemplorum (exempli gratia morbida et temperantia) et re-botrus
Lepidium sativum genes pro singulis botri
Functional annotation and locupletation genes
Differentialis elocutio eiusdem botri inter circulos
Accedit, BMKGENE evolvit "BSTViewer" instrumentum a user-amicum, quo usor ad visualisandam gene expressionem et maculam varias resolutiones conglutinat.
BMKGene progressio pro user amica visualisation software
BSTViewer maculam pampineis in multi-gradu resolutio
BSTCellViewer: latae et manuales cellam scindendi
Interiorem-specimen Analysis
Macula pampineis;
Genus identitatis ac loci distributio titulus:
Inter coetus Analysis
Data complexio ex utroque coetu et re- glomerari;
genesis titulus racemis novis;
Progressiones explorare faciliores per BMKGene operas transcriptomics spatiales cum technologia BMKManu S1000 in hac publicatione featured:
Song, X. et al. (2023) 'Spatialis transcriptomica cellulas chlorenchymatis inductas leves, quae ad regenerationem in callo lycopersico promovendo implicantur, manifestat;Acta Academiae Scientiarum Americae Unitarum120(38), p. e2310163120. doi: 10.1073/pnas.2310163120
Tu, Y. et al. (2023) 'Systematica comparatio methodi transcriptomic sequencing-fundatur spatialis';bioRxivS. p. 2023.12.03.569744. doi: 10.1101/2023.12.03.569744.
