16 * / 18s / eius Amplicon sequencing, NGS
Service Features
● Sequacing Platform: Illumina Novaseq.
● amplificationem brevi regionum de 16s, 18s et ejus, inter se amplificationem peltas.
● flexibilibus electiones Amplicon.
● Previous project experientia cum multiple amplificare peltas.
Service commoda
●Solation libero:Celeri idem Microbial compositionem environmental exempla.
●Princeps resolutio: In humilis-copents in environmental exempla.
●Late applicabiles: Diverse microbial Community Studies.
●Comprehensive Bioinformatic Analysis: Tardus Qiime2 Package (quantitative prudentia in microbial ecology) cum diverse analyses in terms of database, annotation, otu / VULGATE.
●Extensive expertise: Cum CL milia amplicon sequencing projects conducted annuatim, BMKGEGGGGGGGGGGGGGGGE de decade de experientia, a valde peritus analysis quadrigis, comprehensive contentus et optimum post-venditio auxilium.
Service Specifications
| Bibliotheca | Sequencing belli | Notitia commendatur |
| Amplicon | Illumina pe250 | L / C / 300K Tags (Read pairs) |
Service Requirements
| Concentration (ng / μl) | Summa copia (NG) | Volubilis (μl) |
| ≥1 | ≥200 | ≥20 |
● solo / caudem: 1-2g
● intestinorum content-animal: 0,5-2g
● intestinorum contenta insect: 0.10.25G
● Plant Superficiem (Dubratus Efferiment) 0.1-0.5G
● fermentationem ius ditatus ECTUENT): 0.10.5G
● Faeces (Large Animalium) 0.5-2g
● Faeces (mus), 3-5Grains
● pulmonalis alveolaris lavage fluidum: filter paper
● vaginal swab: 5-6 swabs
● cutis / genitalibus swab / saliva / oralis mollis TEXTUS / pharyngeal swab / rectalis swab: 2-3 swabs
● Superficies Microorganisms: Filter Paper
● Waterbody / Aeris / Biofilm: Filter Paper
● endophytes: 1-2g
● Dental Plaque: 0,5-1G
Service opus influunt
Sample partum
Constructio
Sequencing
Analysis
Post-Sale Services
Includit sequenti analysis:
- Rudis data qualis imperium
- Otu clustering / de-strepitu (Clementine_Vulgate)
- Otu annotation
- Alpha diversitas analysis: multiple index, comprehendo Suspendisse, Simpson et Ace.
- Beta diversitas Analysis
- Inter-Group Analysis
- Correlation Analysis inter environmental factores et compositionem et diversitatem
- 16 * eget gene praedictione
Histogram of Taxonomic distribution
Taxonomic abundantiam Percusterum calor map
Alpha Diversity Analysis: Rarefaction curva
Beta Diversity Analysis: NMDS
Intergroup Analysis: Lefse Biomarker Inventionis
Explorarent progressiones facilius per BMKGene scriptor Amplicon sequencing Services cum Illumina per curated collectione publications.
Dong, C. et al. (MMXXII) Conventus, Core Microbiota, et functio ipsarum rhizosphere solo et cortice microbiota in Eucommia ulmoides ', fines in microbiology, XIII. Doi: 10,3389 / FMMMB.2022.855317 / FMMMB.2022.855317 / FMMMB.2022.855317 / Fl.
Li, Y. et al. (MMXXIII) 'synthetial consortia translationi ad curatio de Gardnerella vaginalis-adductus bacterial vaginosis in mures', microbiome, XI (I), pp. 1-14. Doi: 10.1186 / S40168-023-0401497-y
Yang, J., Fu, Y. et Liu, H. (MMXXII) Microbiomes de Aeris pulveris collected in terra-fundatur clausa Biorgenalis vitae Support Experimentum "Lunar Palace CCCLXV" ,, pp. XVII (I), Pp. 1-20. Doi: 10.1186 / s40793-022-00399-0 / figuras / VIII.
Yin, S. et al. (MMXXII), Feedstock-dependens abundantiam genes ad NITROGENIUM transformatio continetur NITROGENIUM damnum in Composting ', Bioseource Technology, CCCLXI, p. (CXXVII) DCLXXVIII. DOI: 10.1016 / J.BIORTECH.2022.127678.
