10x genomics visium spatii transcriptome
Technical machinemur
Features
● Resolutio: C μm
● macula Diameter: LV μm
● numerus maculis: (IV) CMXCII
● captis area: 6.5 x 6.5 mm
● uterque barcoded macula oneratur cum primers composito ex IV sectiones:
- Poly (DT) cauda quia MRNA priming et CDNA synthesis
- Unique Momecular identifier (Umi) ad rectam amplificare Bias
- Spatium Barcode
- Binding Sequence Partial Partial Read I Dimer
● H & E macilentas sectiones
Commoda
●Unum-subsisto ministerium: Integrates omnes experientia et arte-fundatur gradibus, inter crysteo-sectioning, tinguere, TEXTUS Optimization, loci Barcoding, Library Praeparatio, sequencing et Bioinformatics.
● Highly-peritus Technical Team: Cum experientia in super CCL TEXTUS types et 100 species inter humanum, mus, mammal, pisces et plantis.
●Real-vicis update in tota project: Cum plena potestate experimentalem profectum.
●Comprehensive Latin Bioinformatics:Package includes XXIX Analyses et 100+ summus qualitas figuras.
●Nativus notitia analysis et visualization: Available pro diversis investigationis petitiones.
●Ad libitum iuncturam analysis cum uno-cellula mRNA sequencing
Cubits
| Sample Requirements | Bibliotheca | Sequencing belli | Notitia commendatur | Imperium |
| Oct-embedded Cryo Samples (Optimal Diameter: proxime. 6x6x6 MM³) II cuneos per sample | 10x visium CDNA | Illumina pe150 | 50K PE legit per macula (60GB) | Rin> VII |
Nam More Details on Sample praeparatio ductu et servitium workflow, placet sentire liberum loqui adBMKGene Peritus
Opera workflow
In sample praeparatio tempus, an initial mole RNA extractionem iudicii fit ut a summus qualis RNA potest adeptus. In TEXTUS Optimization scaena in sectiones sunt maculata et visualized et permeabilization conditionibus in mRNA dimittere ab TEXTUS sunt optimized. Et optimized protocol est igitur applicari in bibliotheca constructione, sequitur sequencing et notitia analysis.
In completum ministerium workflow involves realis-vicis updates et client confirmationes ad ponere responsive feedback loop, cursus lenis project.
Includit sequenti analysis:
Data Quality Imperium:
O notitia output et qualitas ustulo distribution
O gene deprehendatur per macula
O TEXTUS Coverage
Interiorem-Sample Analysis:
o gene dives
O macula clustering, inter reducta dimensionem analysis
O differentialium Analysis inter clusters: Lepidium sativum Haging Genes
O muneris annotation et locupletantes de titulum genes
Inter-Group Analysis
O rursus ex varietate ex samples (eg. Et control) et re-botro
O idem de titulum genes enim inter botrum portassent
O muneris annotation et locupletantes de titulum genes
O differentialium expressio eiusdem botrus inter coetibus
Interiorem-sample analysis
Clustering macula
Lepidium distribution genes venalicium
Inter-Group Analysis
Data compositum ab utroque coetibus et rursus botrum portassent
Venalicium genes novis clusters
Explorarent progressiones facilitati per BMKGene scriptor spatia transcripomics servitium per 10x visium in his featured publications:
Chen, D. et al. (MMXXIII), MTHL1, A potentiale Drosophila homologue de mammalian adhaesionem GPCRS, quod involved in antittuor reactiones ad infusum oncogenic cellulis in fugit ',Acta de National Academy of Sciences Civitatum Foederatarum Americae, CXX (XXX), p. E2303462120. doi: /10.1073/pnas.2303462120
Chen, Y. et al. (MMXXIII) 'Steel enables summus resolutio delineation of Spatiotemporal transcriptomic notitia',Briefings in Bioinformatics, XXIV (II), pp. 1-10. Doi: 10.1093 / Bib / Bbad068.
Liu, C. et al. (MMXXII) 'a spatiotemporal atlas organogenesis in progressionem orchidorum florum';Nucleic acidis Research, L (XVII), pp. 9724-9737. Doi: 10.1093 / Nar / Gkac773.
Wang, J. et al. (MMXXIII), integrationem spatii transcriptomics et una-nucleus RNA sequencing reveals potentiale medicinales strategies ad vteri Leiomyoma ',Acta Acta Scientiarum, XIX (VIII), pp. 2515-2530. Doi: 10,7150 / ijbs.83510.
