T2T GENOME ASSEMBLY, GAP FREE GENOME
1stLoro Genom Beras1
Irah-irahan: Majelis lan Validasi Loro Genom Referensi Bebas Celah kanggo Beras Xian/indica Nyedhiyakake Wawasan babagan Arsitektur Centromere Tanduran
Doi:https://doi.org/10.1101/2020.12.24.424073
Wektu dikirim: 01 Januari 2021.
Institut: Huazhong Agricultural University, China
Bahan
O. sativa xian / indicavarietas padi 'Zhenshan 97 (ZS97)' lan 'Minghui 63 (MH63)
Strategi urutan
NGS diwaca + HiFi diwaca + CLR diwaca + BioNano + Hi-C
data:
ZS97: 8,34 Gb（~23x）HiFi diwaca + 48,39 Gb (~131x) CLR diwaca + 25 Gb(~69x) NGS + 2 sel BioNano Irys
MH63: 37,88 Gb (~103x) HiFi diwaca + 48,97 Gb (~132x) CLR diwaca + 28 Gb(~76x) NGS + 2 sel BioNano Irys
Gambar 1 Rong genom beras bebas celah (MH63 lan ZS97)
2ndGenom pisang2
Judul: Telomere-to-telomere kromosom tanpa celah pisang nggunakake urutan nanopore
Doi:https://doi.org/10.1101/2021.04.16.440017
Wektu dikirim: April 17, 2021.
Institut: Université Paris-Saclay, Prancis
Bahan
Double haploidMusa acuminatasppmalaccensis(DH-Pahang)
Strategi lan data urutan:
Mode HiSeq2500 PE250 + Minion/PromethION (93Gb,~200X)+ Peta optik (DLE-1+BspQ1)
Tabel 1 Perbandingan rakitan genom Musa acuminata (DH-Pahang).
Gambar 2. Perbandingan arsitektur genom Musa
3rdGenom Phaeodactylum tricornutum3
Title: Telomere-to-telomere genome assembly ofP
haeodactylum tricornutum
Doi:https://doi.org/10.1101/2021.05.04.442596
Wektu dikirim: 04 Mei 2021
Institut: Western University, Kanada
Bahan
Phaeodactylum tricornutum(Koleksi Kultur Alga lan Protozoa CCAP 1055/1)
Strategi lan data urutan:
1 sel aliran minION Oxford Nanopore + output mid-end 2×75 pasangan NextSeq 550
Gambar 3 Alur kerja kanggo rakitan genom telomere-kanggo-telomere
4thGenom CHM13 manungsa4
Judul: Urutan lengkap genom manungsa
Doi:https://doi.org/10.1101/2021.05.26.445798
Wektu dikirim: 27 Mei 2021
Institut: National Institutes of Health (NIH), USA
Bahan: sel line CHM13
Strategi lan data urutan:
30× PacBio circular consensus sequencing (HiFi) , 120× Oxford Nanopore ultra-long read sequencing , 100× Illumina PCR-Free sequencing (ILMN) , 70× Illumina / Arima Genomics Hi-C (Hi-C), peta optik BioNano, lan Strand-seq
Tabel 2 Perbandingan GRCh38 lan T2T-CHM13 rakitan genom manungsa
Referensi
1. Sergey Nurk et al. Urutan lengkap génom manungsa. bioRxiv 2021.05.26.445798; doi:https://doi.org/10.1101/2021.05.26.445798
2. Caroline Belser et al. Telomere-kanggo-telomere kromosom gapless saka pisang nggunakake urutan nanopore. bioRxiv 2021.04.16.440017; doi:https://doi.org/10.1101/2021.04.16.440017
3. Daniel J. Giguere et al. Rakitan genom telomere-kanggo-telomere saka Phaeodactylum tricornutum. bioRxiv 2021.05.04.442596; doi:https://doi.org/10.1101/2021.05.04.442596
4.Jia-Ming Song et al. Majelis lan Validasi Loro Genom Referensi Bebas Gap kanggo Beras Xian / indica Nyedhiyakake Wawasan babagan Arsitektur Centromere Tanduran. bioRxiv 2020.12.24.424073; doi:https://doi.org/10.1101/2020.12.24.424073
Wektu kirim: Jan-06-2022