Ntughari transcrtome - Limina
EGO
● Ọbá akwụkwọ Dualricootome n'usoro nke transcrcome: rrna na-esochi Pe150 na-esonye na nkwadebe ọbá akwụkwọ na-esote nkwadebe ọbá akwụkwọ
Izu ike Bioinformatics nke Mra
Nyochaa nkwonkwo nke ngosipụta RNA niile na mkpesa jikọtara ọnụ, gụnyere nke CNNA netwok.
Uru bara uru
●Nyocha dị omimi nke netwọkụ: A na - eme nyocha nke Cerna netwọọk nke Orna, Lnccrna, Circcna, na Mirna na nke na - agwụ ike na - agwụ ike.
●Nnabata zuru oke: Anyị na-eji ọdụ data ọtụtụ iji kpọpụta mkpụrụ ndụ ihe nketa dị iche iche gosipụtara nke ọma (degs) ma rụọ ọrụ e ji kwekọọ na usoro cellular na molecular na-eme nzaghachi transcartome.
●Nnukwu nkasi ike: Site na ndekọ egwu nke imechi ihe karịrị 2100 transcrcom projectome na ngalaba nyocha dị iche iche, ndị otu anyị na-eweta ọtụtụ ahụmịhe ọrụ ọ bụla.
●Njikwa dị mma: Anyị mejuputara isi njikwa n'akụkụ niile, site na nlele na nkwadebe ọbá akwụkwọ iji sequencenting na bioiofformatics. Nke a na-eleda anya nyochaa nsonaazụ nke nsonaazụ dị oke mma.
●Nkwado Azụmaahịa: Nrara anyị na-agbasa karịrị ọrụ ngwụcha ọnwa atọ. N'oge a, anyị na-enye enyemaka na-esochi, enyemaka nsogbu, na Q & otu oge iji dozie ajụjụ ọ bụla metụtara nsonaazụ
Nlele Nlere Anya na Nnyefe
| Oba akwukwo | Atụmatụ sequencing | Data tụrụ aro | Njikwa Dị Mma |
| rrna depleted | With Wardy Pe150 | 16 gb | Q30 R65% |
| Size ahọpụtara | Ward Wardy Se50 | 10-20m na-agụ |
Ihe nlere anya:
Nucleotides:
| CHAN. (NG / μL) | Ego (μg) | Nko | Eziokwu |
| ≥ 80 | ≥ 1.6 | OD260 / 280 = 1.7-25 OD260 / 230 = 0.5-2.5 Obere ma ọ bụ enweghị protein protein ma ọ bụ ihe mgbochi DNA gosipụtara na gel. | Rin≥6.0 5.0≥28s / 18s≥1.0; Obere ma ọ bụ enweghị ndabere |
Nnyefe nlele tụrụ aro
Akpa: 2 ml centrifuge (tuhi anaghị atụ aro ya)
A na-edebe ihe nlele: Otu + Mepụta EG A1, A2, A3; B1, B2, B3.
Mbupu:
1
2. A ga-akpọtụ ụyọkọ: Enwere ike ịmịchasị RNA na tube RNA (dịka Fnastable®) ma bute ụlọ okpomọkụ.
Ọrụ Ọrụ
Nchọpụta nnwale
Nyefe Inye Aka
Mmịpụta RNA
Ọbá Akwụkwọ Ewu
Sequencing
Nyocha data
Ọrụ Ahịa
Bioniekatics
Nyochaa RNA
Mkpụrụ ndụ mkpụrụ ndụ ochie
Cerna nyocha
Emechaala Minas na Flas
Nyochaa nyocha nyocha ahụ site na ọrụ transcriptome site na nchịkọta transcrctome site na nchịkọta nke akwụkwọ.
Dai, y. et al. (2022) profaịlụ nke MRNAS, LNCRNAS na Mirnas na ọrịa Kashin-Beckifing nke RNA na-achọpụta ', molecular rụrụ arụ, 184-166. DI: 10.1039 / D1MO00370D.
Liu, n. naan et al. (2022) 'Nyochaa ogologo transcriples nke ngụgide nke APIS Cerana na Chadbai Ọbabeghị.' 146503-14603. DI: 10.1016 / J.GENE.2022.146503.
Wang, xj et al. (2022) 'Ntinye aka na-ejikọ aka na-asọmpi nke netwọọk na-asọmpi na obere cansung cancer cancer: ihe ndị na-eme ọgwụ na-agagharị, na-ebu ibu na incology, 12, p. 904865. DI: 10.3389 / fonc.2022.904865 / Bibtx.
XU, P. et al. (2022) 'Nnyocha nke LNCCo / CIRCo-Mirna-Micatodes na-ekpughe ihe ọmụmụ ihe na-egosi na ahụenatodes na ahụekere', nke Bmc, 23), pp. 1-12. 1-12. DI: 10.11186 / s12864-022-08470-3 / onu ogugu 7.
Yan, Z. et al. 2022) Otu ụzọ transcartome na-egosiputa usoro molecular na mmezi nke mmachi nke postharsi site na Red Enges nke Red na teknụzụ, 188, p. 111878. Mee: 10. 10.1 / J.OSTHARVBIO.2022.111878.
