Geneme Bisul Fi na-achọ (WGBS)
Ihe omumu ihe omumu (WGBS) guzo dika usoro nke di omimi nke DNAME na Cytosine (5-MC), onye na-ekwu okwu di nma nke ihe omuma na oru ngo. Disekpụrụ ahụ na-enye WGBS na-agụnye ọgwụgwọ, na-eme ka ntụgharị nke cytosian na-enweghị atụ (C ka ị na-agbanweghi methylines. Usoro a na - enye ndị na - eme nchọpụta mkpebi, na - enye ohere inyocha Methylome ma kpughee usoro methylation metụtara ọnọdụ dị iche iche, ọ bụ ọrịa kansa. Site na iji WGB, ndị ọkà mmụta sayensị nwere ike inweta nghọta na-enweghị atụ n'ime ọdịdị mkpụrụ ndụ mkpụrụ ndụ, na-enye nghọta nke usoro epigenitic na ọrịa dị iche iche.
Atụmatụ Ọrụ
● chọrọ mkpụrụ ndụ Kenome.
A na-agbakwunye DAMDA DNA iji nyochaa arụmọrụ ntụgharị bisulfite.
● Cowquing na Ward Lightq.
Uru bara uru
●Gold Gold maka nyocha DNA: Nke a na-eme ka teknụzụ ntụgharị methylation nwere ezi omume na ezigbo ịmụgharị.
●Ebe a na-enwe sara mbara na otu isiNchọpụta nke saịtị methylation na ọkwa mkpụrụ osisi mara mma.
●Nkwụghachi zuru oke:Nye otu nkwụsị ọrụ dị mma site na nhazi nlele, owuwu ọba akwụkwọ, oge na nyocha Bioinformatics.
●Nnukwu nkasi ike: Site na WGBS Seaquing Projection nke zuru oke n'ofe dị iche iche, BMKGENENT na-eweta afọ iri, otu egwuregwu nyocha kachasị mma, na ezigbo nkwado na-akwado ahịa ahịa.
●O nwere ike isonye na nyocha transcriptic: Nyefee maka nyocha nke WGBS na data Okike ndị ọzọ dịka RNA.
Nkọwapụta nlele
| Oba akwukwo | Atụmatụ sequencing | Mmepụta data a tụrụ aro | Njikwa Dị Mma |
| Bisilfite mesoro | With Wardy Pe150 | 30x omimi | Q30 ≥ 85% Ntughari binsilfite> 99% |
Nlele Nyere
| Ileta (NG / μl) | Ọnụ ego (μg) | Ihe ndị ọzọ chọrọ | |
| Gnamic DNA | ≥ 5 | ≥ 400 ng | Mmebi iwu ma ọ bụ mmerụ ahụ |
Ọrụ Ọrụ
Nyefe Inye Aka
Mmịpụta DNA
Ọbá Akwụkwọ Ewu
Sequencing
Nyocha data
Nnyefe data
Gụnyere nyocha ndị a:
Controning Njikwa ịdị mma dị mma;
Ịdịnye aka na mkpụrụ edemede;
● chọpụta ntọala 5mc methylass;
Nchịkọta nke nkesa Methelling na dennetation;
● Nyochaa mpaghara methy dị iche iche (DMRS);
● Esemokwu na-arụ ọrụ nke mkpụrụ ndụ ihe nketa na DMRs.
Nchọpụta 4MC Methylation: ụdị saịtị methylas
Methylition map. 5MC methylation na-ekesa
Akwukwo nke oma nke di iche iche methlem
Mpaghara metylated dị iche iche: mkpụrụ ndụ nwere njikọ
Mpaghara metylas dị iche iche: nnabata nke mkpụrụ ndụ ihe nkekọ (Gene nta)
Nyochaa nyocha nyocha ahụ site na ọrụ ọmụmụ BMKGOME niile site na nchịkọta nke akwụkwọ.
Fan, y. et al. (2020) 'Nyocha nke DNA Methylition n'oge atụrụ nke anụ ụlọ ọkpụkpụ site na iji mkpụrụ ndụ na-egbu egbuBMC Gonnamics, 21 (1), pp. 1-15. DI: 10.1186 / s12864-020-6751-5.
Zhao, x et al. (2022) 'Akwụkwọ ọgụgụ dexyribonucleic acids ike na ndị ọrụ gosipụtara vinyl chloride',Toxicology na ahụike, 38 (7), pp. 377-388. DI: 10.177 / 07482337221098600
Zuo, j. et al. (2020) 'Mmekọrịta dị n'etiti Genome methylition, ọkwa nke na-abụghị nke zoro ezo, Mrnas na Mitabolites na-acha tomato ripening mkpụrụ osisi',Jourcesnye osisi, 103 (3), pp. 980-994. DOI: 10.111 / TPJ.14778.
