TGuide S16 nucleic Acid Extractor
Nmkpọ Okwu mmalite
TGuide S16 nucleic acid nwere ike hazie ihe ruru 16 maka mkpanaka magnetik ka ọkwà magnetik magnetik ma ọ bụ otu na-ahụ maka ndọta. Site n'iji ihe ndị na-ede ihe na-ede akwụkwọ na-eme nke ọma, ngwaahịa a nwere ike ịmịpụta DNA / Acteria, Ducteria, wdg, ma wepụ DNA site na plasmid, Agarose Gel na Pcr ngwaahịa.
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EGO
Super mfe iji
Ọ dịghị ọrụ ọkpọkọ. Enwere ike idozi olu ịlegharị anya dịka achọrọ.
Nwụnye na ọrụ chọrọ obere ọzụzụ. Site na mmemme awụnyere tupu awụnyere ya, wezụga ngwa azụ, họrọ ogbugba ndu ma na-agba ọsọ. Mmemme dị njikere iji ya, yanakwa ọdịiche.
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Ọhụụ Ọhụrụ
Mkpanaka magnetiki na-agagharị na njedebe njedebe siri ike.
Ihe mkpuchi ndọta na mkpanaka ala ahụ, nke na-eme ka ihe niile dị larịị magnetik niile mgbe olu dị obere. Ihe eji emepụta nke mkpụrụ nke nucleic acid.
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Ezigbo imewe nke igwe na-ekpo ọkụ
Enwere otu kọlụm n'etiti ihe na-ahụ maka lysis na-egbochi ihe niile site na iji kpo oku. Ya mere, olu mgbake nke nucleic acdard ga-ezikwu eziokwu.
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Njikwa mmeru na njide nche
Enwere ike itinye ya na bench dị ọcha ma ọ bụ na mmiri ozuzo.
Ihe eji eme ihe na uzo akpaka.
Sistemụ a na-egbochi usoro abụọ: usoro njikwa ihe na-egbochi ya na sistemụ na-egbochi imegharị, iji zere mmerụ ahụ n'etiti ogidi na ihe nlere.
Ndị na-ede ego na-ere ahịa ma kwekọọ.
A na-ewepụkwa njikwa kemịkalụ ruo na nchekwa kachasị mma.
Nsonaazụ ngosi
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Nnwale: Iji TGuide S16 na Regagent TGUID Smart Small Universal Dna Kit (4993704)
Iji wepụta ụma nke mkpụrụ osisi gụnyere mkpụrụ osisi gụnyere mkpụrụ ndụ, anụ ahụ, ọbara, ọgbụgba, na swab, wdg, na-enweta ezigbo mkpụrụ na ịdị ọcha.
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Nke OD260 / OD280 gburugburu 1.7 ~ 1.9, na
OD260 / OD230> 1.6.
Olu elu: 100 μl
Agarose gel ịta: 1.5%
Inye elu: 1 μl
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Iji TGuide S16 na Regagent TGUID Smart Smart DNA / RNA (4993702)
Iji wepụta DNA / RNA site na ọtụtụ ebe gụnyere ọbara niile, swab na plasma ga-enweta ezigbo mkpụrụ na ịdị ọcha dị mma. -
OD260 / OD280 gburugburu 1.7 ~ 1.9, na OD260 / OD230> 1.6.
Companylọ ọrụ ụlọ ọrụ & Bọdị ama ama ama ama.
ND: Ọrịa Newcastle, H5: Ọrịa Avian, CP: Canine Parvovirus, ASF: Nje Virus Africa Sallovirus
Ihe omuma kacha nta nwere ike iru 100 mbipụta / ml.
Nnwale: Iji TGuide S16 na Regent TGUID Smart Smamic Smamic DNA Kit na-eme ka mkpụrụ obi na-akpaghị aka, nke kwekọrọ na mkpụrụ osisi na ịdị ọcha nke ngwaahịa a ma ama.
OD260 / OD280 gburugburu 1.7 ~ 1.9, na OD260 / OD230> 1.7.
Isi mmalite: Ihe nlere atọ nke ọbara. Atọ na-atụgharị uche maka otu ngwaọrụ.
Mejupụta maka kit nke ọ bụla sitere n'otu ebe nlele.
Ọnọdụ nlele: Ọbara akpọnwụ
Ntinye olu: 200 μl
Olu elu: 100 μl
Agarose gel ịta: 1.5%
Inye elu: 1 μl
Nna-ukwu: Marker III, Tiangen
Companylọ ọrụ ụlọ ọrụ & B.Ndị ama ama ama ama
2000 BP nke gara agarose Gel Dification na mgbake
Agarose gel ịta: 1.5% (TBE)
Olu nke: 8 μl
Marker: D2000, Tiangen
S16-01, S16-02, S16-03 na-anọchi anya ọdịnala Prilel na nnwale nke mgbake site na TGuide Smart Kit (4993550) n'otu oge.
Otu ụlọ ọrụ: Akwụkwọ ntuziaka na kọlụm akwụkwọ mmịpụta nke ụdị ama ama ama ama ama ama ama
A na-eji ihe ruru afọ 2100 maka nchọpụta iguzosi ike n'ezi ihe.
Isi iyi nke: CD-1 Nice
Ọnọdụ nlele: ọbara zuru oke
Ogo nha: 200 μl
Nsonaazụ: Ngụkọta RNA wepụtara site n'ọbara site na TGuide S16 nke nwere ọbara / cell / anụ ahụ na-enwe ezi mkpụrụ na ịdị ọcha nke mgbada site na usoro nke mgbada.
Olu na-eburu: 1 μl. Emetụta imeju ahụ ugboro ise ma buru ibu. Agarose gel ịta: 1%. Electrophoresis maka 20 min na 6 v / cm;
M: Tiangen Marker III
1-4: Obi 5-8: Ume 9-12: Ngụgụ 13-16: akụrụ
Ihe nlere abuo nke nke aru nke ọ bụla site na mkpụrụ osisi na-esite na ngwa, na nke ikpeazụ na nke ikpeazụ wepụtara na TGuide S16 na akpaghị aka site na TGuide S16.
Isi iyi nke: CD-1 Nice
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NSOGBU NIILE: A na-enweta ihe nyocha: TGuide S16 kwadebere ya na-akpaghị aka na Regent TGUID Smart Scark DNA Kit (4993547) Mkpụrụ DNA wepụtara na mmịpụta nke kọmpụta na-enweghị atụ. Ọdịmma ahụ bụ otu ihe ahụ, na OD260 / OD280 gburugburu 1.7 ~ 1.9 na OD260 / OD230> 1.7. Na mmechi, enwere ike iji TGuide S16 wepụta ihe sitere na anụ ahụ nke mkpụrụ ego site na nnọchi nke ihe atụ-kọlụm.
Ngụkọta RNA site na ihe nlere dị iche iche
Nha anya: 100 mg
Nyochaa tupu oge okpomọkụ dị ala
Agarose gel ịta: 1% (tae)
Inye elu: 1 μl
Nna-ukwu: Marker III, Tiangen
1-4: ọka 5-8: ọka doo 9-12: Ngụgụ nke 13-16: soybeans
Ewepụtara ihe nlere abụọ nke mbụ site na pịtụ-kọọ na kọlụm mkpụrụ osisi na ihe nlere abụọ ikpeazụ e wepụtara ya site na TGuide S16.
Mkpụrụ osisi nke Genic DNA
Nha anya: 100 mg
Ihe atụ maka ọgwụgwọ: egwe mmiri mmiri ma ọ bụ anụ ahụ na-egweri homogenizer
Agarose gel ịta: 1% (tae)
Inye elu: 2 μl
Marker: D15000, Tiangen
Ụlọ ọrụ: ụdị ama ama ama ama
Nnyocha: Iji TGuide S16 na Regagent TGuide Smart Smart Smart STAN Kit na-akpaghị aka nke mkpụrụ osisi nke Genomic na-akpaghị aka, nke ya na mkpụrụ mmịpụta na ịdị ọcha nke ndị a maara nke ọma. Ọzọkwa, iguzosi ike n'ezi ihe nke Genic DNA dị mma karịa nke a na-asọ asọ nke ụlọ ọrụ ndị ọzọ. Na nnwale a, a na-ewepụta 15 μg nke mgbọrọgwụ ọka, na OD260 / OD280 / OD28, na OD260 / OD230> 2.0.
