Exclusive Agency for Korea

Bmkcloud abanye na

Ngwaahịa

Otu- nuclei RNA Sequencing

Mmepe nke cell cell na usoro owuwu ụlọ akwụkwọ na-ewu ọba akwụkwọ, tinyere usoro ntinye elu, agbanweela ọmụmụ okwu doro anya na ọkwa Cell. Ihe a na - eme ka ihe dị omimi na - eme nyocha dị omimi nke ọnụ ọgụgụ ndị dị mgbagwoju anya nke ọnụ ọgụgụ ndị dị mgbagwoju anya, na - eme ihe mgbochi ndị metụtara ya na usoro ngosipụta nke mkpụrụ ndụ niile na iche iche bi na ya. Ọ bụ ezie na otu-sel rna sel (skụne-seq) nwere uru a na-apụghị ịgbagha agbagha, ọ na-ezute ihe ịma aka n'ọtụtụ oge na-egosi na ọ chọrọ nlele ọhụụ. Na BMKGGENE, anyị na-elebara nsogbu a site na ịnye ndị otu-nuce-nuce-nuques RNA (SNNA-SEQ) site na iji teknụzụ Tratics Art 10x. Akụkụ a na-eme ka ụdị ụdị nlele nke ihe nlele na nyocha transcrictor na otu-sel.

A na-arụpụta iche na nuclei na-emezu site na nyocha nke 10x ọhụrụ, na-egosipụta sistemụ microfluids asatọ na obe abụọ. N'ime usoro ihe a, gel beads na-etinye barcodes, ndị na-eme, enzymes, na otu nucleus na-etinye mmanụ nanoliter na-ada, na-etolite gel-in emulsion (gem). Followinggbaso usoro gem, cell yysis na akara ngosi aka na-eme n'ime osisi ọ bụla. Emechara, Mrna Molecules na-emegharị ntụgharị na Cdnas, tinye akara 10x na njirimara mlacular pụrụ iche (Umus). A na-edobe CDna a na-ewu ụlọ akwụkwọ ọba akwụkwọ, na-eme nyocha siri ike ma na-enyocha profaịlụ nke Gene na otu-sel ọkwa.

Ikpo okwu: 10 × atomis Chrim na Wind Wind Novaseq

Kpọtụrụ anyị

Nkọwa ọrụ

Bioniekatics

Nsonaazụ ngosi

E gosipụtara akwụkwọ

Usoro teknụzụ

A na-enweta mwepu nke nuclei site na 10 ×Ní mkpụrụ osisi Chrimium ™, nke mejupụtara microfluids sistemụ nwere ụzọ abụọ. Na usoro ihe a, ihe gel nwere barcodes na pricer, enzymes na otu nucleus na-etinye na mmiri nanolineus na-eme ka ọ dị, na-emepụta). Ozugbo gem guzobere, cell lysis na mwepụta barcodes na gem ọ bụla. Mrna bụ ntụgharị nke CDNA na 10 ×Dodes na UMi, nke na-etinyekwu isi na mpaghara ọba akwụkwọ.

EGO

Nkwadebe nke Nkwụsịbe-nuclei site na anụ ahụ jụrụ oyi

● Ẹkedode gel bead-emulsion (gem) CDNA sochiri

● Bead ọ bụla bead ọ bụla na gem na-eburu ya na primars mejupụtara 4 ngalaba:

Poly (DT) Orna Play na Cdna,

Njirimara molecular (UMI) iji mezie nmebi

10x barcode

Na-ekekọta usoro nke otu akụkụ nke Primer

Uru

Otu-nucleus RNA na-ebute erughị mma nke otu-cell rna sel, na-enyere aka:

● Ojiji nke ihe nlele na-eme ihe na-adịghị mma ma ọ bụghị naanị na sọọsọ ọhụrụ

● Ọnụ ala mkpụrụ ndụ kpọrọ nkụ ma e jiri ya tụnyere ọgwụgwọ nke sel ọhụrụ, gosipụtara site na data transcrcome n'ụdị mkpụrụ ndụ ihe nketa

● Ọ dịghị mkpa maka iwepụ sel ọbara ọbara

● dayameta anaghị akpa ókè

● Otu nnukwu ihe nlere nke tozuru oke maka nyocha, gụnyere ụdị ihe ndị na-adịghị mma na-eme ka ihe ndị na-adịghị mma na-eme ka cell na-eme mkpọtụ ma ọ bụ mbibi

A na - enwe ike nyocha site na otu-cell rna sel na tozuru oke maka otu nuclei RNA Sequence:

Sel / anụahụ	Ucheobi
A na-ejikọ anụ ahụ akpọnwụ	Enweghi ike inweta òtù echekwara ma ọ bụ ogologo
Celllullul, megakaryocyte, abụba ...	Mpempe akwụkwọ ahụ buru oke ibu iji tinye ngwa ahụ
Imeju ...	Na-esighi ike iji mebie, enweghị ike ịmata otu mkpụrụ ndụ
Neuron sel, ụbụrụ ...	Ndị ọzọ na-emetụ uche karịa, ọ ga-adị mfe nrụgide, ga-agbanwe nsonaazụ sequng
Pa pancreas, thyroid ...	Ọgaranya na enzymes enbigenous, na-emetụta ịmepụta mmepụta nke otu nkwụsị

Otu-nucleus vs otu-sel

Otu-nucleus	Otu - sel
Dayameta na-akparaghị ókè	Mpempe akwụkwọ nke sel: 10-40 μm
Ihe nwere ike ịbụ anụ ahụ jụrụ oyi	Ihe ahụ ga-abụrịrị anụ ahụ
Ọnọdụ nrụgide nke mkpụrụ ndụ jụrụ oyi	Ọgwụ enzyme nwere ike ibute mmeghachi omume cell
Enweghị mkpụrụ ndụ ọbara ọbara ọ bụla	Ewepu sel ọbara ọbara
Nuklia na-ekwupụta bioinformation	Mkpụrụ ndụ niile na-ekwupụta bioinform

Nkowa

Nlele Nyere

Oba akwukwo

Atụmatụ sequencing

Data tụrụ aro

Njikwa Dị Mma

Anụ ahụ anụmanụ ≥ 200 mg

Njirimara ọkụkụ ≥ 400 mg

10x

With Wardy Pe150

100k na-agụ otu sel

(100-200)

700-1200 nuclei / μl na iguzosi ike n'ezi ihe Nuclei

Maka nkọwa ndị ọzọ na Nduzi nkwadebe na ọrụ ọrụ, biko nweere onwe gị ịgwa ndịBMKGEENENENENE

Ọrụ Ọrụ

Nke gara aga: Osisi / Anụmanụ dum Genome Sequng

Osote: Nyochaa mkpakọrịta mkpụrụ ndụ mkpụrụ ndụ

Gụnyere nyocha ndị a:

Njikwa nke mma: Ọnụ ọgụgụ nke sel, nchọpụta mkpụrụ ndụ mkpụrụ ndụ, njirimara nke sel, RNA molecules na nkọwapụta

Nnyocha nke anyị:

Cell comtumering na ụyọkọ ụyọkọ

Nyochaa ngosipụta: njirimara nke degs na ụyọkọ

Mmezu ọrụ na ebumnuche nke ụyọkọ ụyọkọ

IJI NKE EGO:

Njikọ data

Nyocha dị iche iche: njirimara nke degs na otu

Na-arụ ọrụ na ebumnuche nke degs

Nnyocha dị elu:

Nyocha Cell Cell

Nyocha pseudotim

Nyocha Nkwukọrịta Cell (Cellphonedb)

Gene setịpụrụ nyocha nyocha (GSEA)

Nyocha nke ime

Cellgbọ elu:

Nyocha dị iche iche: ụyọkọ ụyọkọ

Nnyocha nke otu

Nyochaa ngosipụta: otu degs

Nyochaa:

Nnyocha pseudotime:

Mkpokọta Cell:

Nyochaa ọdụ ahụ na-eme ka ọ bụrụ otu na-ahụ maka otu-nucleus Rna Sequences site na 10x chromium na akwụkwọ ndị a gosipụtara:

Wang, L. et al. (2021) 'Nnyocha nke Centerc Plan na-ekpughe oghere ahụ na-egbochi ngụgụ na skoid-na-eguzogide ọgwụ',Usoro nke obodo Academy nke United States of America, 118 (2), p. E20055901118. DI: 10.1073 / pnas.20055901111

Zheng, H. et al. (2022) 'network regroulary maka ngosipụta nke ihe ọmụmụ ihe na-egbochi ihe na-egbochi mkpụrụ ndụ na-egbochi ya na microninoto nke rọrọ nke',Fisters na immunology, 13, p. 879824. DI: 10.3389 / fimmu.2022.879824 / BibTex.

Tian, H. et al. (2023) 'A na-ejighị ike nke na-enweghị ike imegharị ya na-enweghị ike ịmegharị ya na-eme nyocha nke leukocytes mgbe ịgba ọgwụ mgbochi Edwantiel na Fardọ Edwacta na Frive (paralichthys olivaceus)',Agatucture, 566, p. 7392388. Doo: 10. 10.16 / J.AQuaculture.2023.739238.

YU, Y. ET al. (2023) 'Usoro ọgwụgwọ photodnam na-eme ka nsonaazụ ya na-egbochi ndị na-egbochi ihe mgbochi site na ihe mgbochi na-egbochi ọgụ na ndị ọrịa nwere ọrịa kansa na-emegharị',Gastric cancer, 26 (5), Pp. 798-813. DI: 10,1007 / s10120-023-01409-x / metrik.