TRAMaticy RNA
EGO
● Rịmpị nlele banyere Rrna depleten nke Nkwadebe Ranna.
Nnyocha Bioinnatic nke sitere na itinye akwụkwọ na ederede Key
Actụ nyocha na degs na degs na nyocha SRNNA
Uru bara uru
●Njikwa dị mma: Anyị mejuputara isi njikwa n'akụkụ niile, site na nlele na nkwadebe ọbá akwụkwọ iji sequencenting na bioiofformatics. Nke a na-eleda anya nyochaa nsonaazụ nke nsonaazụ dị oke mma.
●Data dị iche iche: N'ihi nkwadebe nke RNA na-akwado itinye aka na mpaghara, na-enyere njirimara nke transcript nke mgbochi.
●Ngosiputa zuru ezu na proparyotic: Pioinforfornine pipeline gụnyere nyocha nke okwu ihe nketa kama nyocha nke usoro transcript, gụnyere njirimara nke arụmọrụ, utrs na ndị na-akwalite. Ọ gụnyekwara nyocha nke SRNAS, ya bụ, na-emepụta na amụma banyere ihe abụọ na ebumnuche.
●Nkwado Azụmaahịa: Nrara anyị na-agbasa karịrị ọrụ ngwụcha ọnwa atọ. N'oge a, anyị na-enye enyemaka na-esochi, enyemaka nsogbu, na Q & A na-ekwu maka ajụjụ ọ bụla metụtara nsonaazụ ya.
Nlele Nlere Anya na Nnyefe
| Oba akwukwo | Atụmatụ sequencing | Data tụrụ aro | Njikwa Dị Mma |
| rrna depleted ọbá akwụkwọ | With Wardy Pe150 | 1-2 GB | Q30 R65% |
Ihe nlere anya:
| CHAN. (NG / μL) | Ego (μg) | Nko | Eziokwu |
| 50 | 1 | OD260 / 280 = 1.8-2.0 OD260 / 230 = 1.0-2.5 Obere ma ọ bụ enweghị protein protein ma ọ bụ ihe mgbochi DNA gosipụtara na gel. | Rin≥6.5 |
Nnyefe nlele tụrụ aro
Akpa: 2 ml centrifuge (tuhi anaghị atụ aro ya)
A na-edebe ihe nlele: Otu + Mepụta EG A1, A2, A3; B1, B2, B3.
Mbupu:
1
2. A ga-akpọtụ ụyọkọ: Enwere ike ịmịchasị ụrọ:
Ọrụ Ọrụ
Nyefe Inye Aka
Ọbá Akwụkwọ Ewu
Sequencing
Nyocha data
Ọrụ Ahịa
Nchọpụta nyocha Bioininformatic
Gụnyere nyocha ndị a:
Njikwa data dị mma
Denye aha ya
Ntụle ọbá akwụkwọ: Condotion RONA RANDETEL, tinye nha na oge ntoju
● Mmezi akụkọ banyere mkpụrụ ndụ ihe nketa
Nyochaa Nkwupụta: Nchịkọta Mmekọ na Nchịkọta Nchịkwa (PCA)
● Okwu ederede dị iche iche (degs)
● Ọrụ na-arụ ọrụ na ebumnuche nke degs
Accchọpụta Sarna: Amụma, na-emekọ ihe, ebumnuche, na nke abụọ
Nnyocha edepụtara: igwe, bido na njedebe na-ejedebe ọnọdụ, mpaghara adịghị agbanwe agbanwe (UTS), Onye na-akwalite
Oge Saquencing
Na-arụ ọrụ na-arụ ọrụ nke mkpụrụ ndụ ihe nketa
Mmekorita n'etiti nlele
Ihe omuma di iche iche ekwuputara (degs)
Nchọpụta enrity
SRNA
Nyochaa ọdụ ahụ na-eme ka ọrụ nke BMKGENENENENENENENTE zuru oke na mbipụta a gosipụtara.
Guan, CP et al. (2018) 'Redcry transcrtome nke biofilm-na-etolite staphylococcus exedermidis na-aza Alkaloids nke Sophorrooides',Akwụkwọ Polish nke microbiology, 67 (2), p. 223. DI: 10.21307 / pjm-2018-024.
