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DNBSEQ ụlọ akwụkwọ emeburu
DNBSEQ, nke MGI mebere, bụ teknụzụ NGS ọhụrụ nke jisiri ike belata n'ihu ọnụ ahịa usoro na ịbawanye mmepụta. Nkwadebe nke ọba akwụkwọ DNBSEQ gụnyere nkewa DNA, nkwadebe nke ssDNA, na mmụba okirikiri iji nweta DNA nanoballs (DNB). A na-ebunye ihe ndị a n'elu elu siri ike ma mechaa soro ya site na ngwakọta Probe-Anchor Synthesis (cPAS). Teknụzụ DNBSEQ na-ejikọta uru dị n'inwe mpe mpe mpe mpe mpe mpe akwa yana iji ụkpụrụ njehie njupụta dị elu na nanoballs, na-ebute usoro na ntinye dị elu na izi ezi.
Ọrụ usoro ọba akwụkwọ anyị emeburu na-enyere ndị ahịa aka ịkwadebe ọba akwụkwọ nke Illumina sitere na isi mmalite dị iche iche (mRNA, genome dum, amplicon, ọba akwụkwọ 10x, n'etiti ndị ọzọ), nke agbanwere na ọba akwụkwọ MGI n'ime ụlọ nyocha anyị ka edobere ya na DNBSEQ-T7, na-enyere aka. nnukwu data ego na ọnụ ala.
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Illumina emeburu ọba akwụkwọ
Teknụzụ usoro nke Illumina, dabere na Sequencing site Synthesis (SBS), bụ ihe ọhụrụ NGS nabatara n'ụwa niile, na-ahụ maka imepụta ihe karịrị 90% nke data usoro usoro ụwa. Ụkpụrụ nke SBS na-agụnye ịse foto n'ụdị fluorescent nke a na-akpọ reversible terminators ka agbakwunyere dNTP ọ bụla, ma mesịa kewaa iji kwe ka ntinye nke ntọala na-esote. N'ebe dNTP anọ niile na-atụgharị akwụsị akwụsị dị na usoro usoro nke ọ bụla, asọmpi eke na-ebelata mbuso agha. Teknụzụ a na-agbanwe agbanwe na-akwado ma ụlọ akwụkwọ ọbá akwụkwọ na-agụ otu na nke jikọtara ọnụ, na-enye ọtụtụ ngwa genomic. Ikike dị elu nke Illumina sequencing na nke ziri ezi tinye ya dị ka isi nkuku na nyocha nke genomics, na-enye ndị ọkà mmụta sayensị ike ka ha kpughee mgbagwoju anya nke genome nwere nkọwa na arụmọrụ enweghị atụ.
Ọrụ usoro ọba akwụkwọ anyị emeburu na-enyere ndị ahịa aka ịkwadebe ọba akwụkwọ usoro sitere na isi mmalite dị iche iche (mRNA, genome dum, amplicon, ọba akwụkwọ 10x, na ndị ọzọ). N'ikpeazụ, enwere ike ibuga ọba akwụkwọ ndị a gaa na ebe a na-ahazi usoro anyị maka njikwa mma na nhazi usoro na nyiwe Illumina.
