-
Usoro metagenomic -NGS
Metagenome bụ mkpokọta mkpokọta mkpụrụ ndụ ihe nketa nke obodo agwakọtara dị ka metagenomes gburugburu ebe obibi na mmadụ. O nwere genome nke ma microorganisms a na-akụ mkpụrụ na nke a na-apụghị ịkụ mkpụrụ. Shotgun metagenomic sequencing na NGS na-enyere aka ọmụmụ nke ndị a mgbagwoju anya genomic Okirikiri ala agbakwunyere na gburugburu ebe obibi samples site na-enye ihe karịrị taxonomic profiling, na-enye kwa granular nghọta n'ime ụdị dị iche iche, n'ụba ike ike, na mgbagwoju ọnụ ụlọ owuwu. Na agafe ọmụmụ taxonomic, shotgun metagenomics na-enyekwa echiche genomics na-arụ ọrụ, na-enyere aka nyocha nke mkpụrụ ndụ ihe nketa na ọrụ ntinye ha na usoro gburugburu ebe obibi. N'ikpeazụ, nguzobe nke netwọk mmekọrịta n'etiti ihe mkpụrụ ndụ ihe nketa na ihe ndị metụtara gburugburu ebe obibi na-enye aka na nghọta zuru oke nke mkparịta ụka dị mgbagwoju anya n'etiti obodo microbial na ebe obibi ha. N'ikpeazụ, usoro metagenomic guzoro dị ka ihe dị mkpa maka ikpughe ihe omimi nke mkpụrụ ndụ ihe nketa dị iche iche, na-eme ka mmekọrịta dị n'etiti mkpụrụ ndụ ihe nketa na gburugburu ebe obibi dị n'ime gburugburu ebe obibi dị mgbagwoju anya.
Platforms: Illumina NovaSeq na DNBSEQ-T7
-
Usoro metagenomic-TGS
Metagenome bụ mkpokọta mkpụrụ ndụ ihe nketa nke obodo agwakọta dị ka metagenomes gburugburu ebe obibi na mmadụ. O nwere genome nke ma microorganisms a na-akụ mkpụrụ na nke a na-apụghị ịkụ mkpụrụ. Usoro metagenomic na-enyere aka n'ịmụ ihe gbasara mbara ala ndị a gbagwojuru anya agbakwunyere n'ime ihe atụ gburugburu ebe obibi site n'inye ihe karịrị nkọwa taxonomic. Ọ na-enyekwa echiche genomics na-arụ ọrụ site n'ịchọgharị mkpụrụ ndụ ihe nketa etinyere na ọrụ ntinye ha na usoro gburugburu ebe obibi. Ọ bụ ezie na a na-eji usoro égbè ọdịnala na-abịaru nso na usoro Illumina n'ọtụtụ ebe na ọmụmụ metagenomic, ọbịbịa nke Nanopore na PacBio usoro ịgụ ogologo oge agbanweela ubi ahụ. Teknụzụ Nanopore na PacBio na-eme ka nyocha bioinformatic dị ala, ọkachasị mgbakọ metagenome, na-eme ka mgbakọ na-aga n'ihu. Akụkọ na-egosi na metagenomics dabere na Nanopore na PacBio ewepụtala mkpụrụ ndụ nje bacteria zuru oke na mechiri emechi site na microbiomes dị mgbagwoju anya (Moss, EL, et al., Nature Biotech, 2020). Ijikọ Nanopore na-agụ ya na Illumina na-agụ na-enye usoro atụmatụ maka mgbazi njehie, na-ebelata izi ezi dị ala nke Nanopore. Ngwakọta mmekọrịta a na-eme ka ike nke usoro usoro nke ọ bụla, na-enye ngwọta siri ike iji merie njedebe ndị nwere ike ime na ịkwalite nzizi na ntụkwasị obi nke nyocha metagenomic.
Platform: Nanopore PromethION 48, Illumina na PacBio Revio
-
Usoro genome bisulfite dum (WGBS)
Genome Bisulfite Sequencing (WGBS) na-eguzo dị ka usoro ọkọlọtọ ọla edo maka nyocha miri emi nke DNA methylation, kpọmkwem ọnọdụ nke ise na cytosine (5-mC), onye na-achịkwa mkpụrụ ndụ ihe nketa na ọrụ ekwentị. Ụkpụrụ dị n'okpuru WGBS na-agụnye ọgwụgwọ bisulfite, na-eme ka ntụgharị nke cytosin na-adịghị edozi na uracil (C ruo U), ebe ọ na-ahapụ cytosin methylated agbanweghị. Usoro a na-enye mkpebi naanị otu, na-enye ndị nyocha ohere inyocha methylome nke ọma wee kpughee usoro methylation na-adịghị mma metụtara ọnọdụ dị iche iche, ọkachasị kansa. Site n'iji WGBS were, ndị ọkà mmụta sayensị nwere ike nweta nghọta na-enweghị atụ na mbara ala methylation genome, na-enye nghọta dị nro nke usoro epigenetic nke na-ebute usoro na ọrịa dị iche iche.
-
Nyocha maka ntụgharị-Chromatin nwere ike ịnweta ya na usoro ntinye dị elu (ATAC-seq)
ATAC-seq bụ usoro usoro ntinye dị elu ejiri maka nyocha nnweta chromatin obosara. Ọ na-eji na-enye nghọta miri emi nke usoro mgbagwoju anya nke njikwa epigenetic zuru ụwa ọnụ gbasara mkpụrụ ndụ ihe nketa. Usoro a na-eji transposase Tn5 hyperactive n'otu oge ma kpado mpaghara chromatin mepere emepe site na itinye ihe nkwụnye usoro. Mgbakwunye PCR na-esote na-arụpụta ịmepụta ọbá akwụkwọ usoro, nke na-enye ohere maka nchọpụta zuru oke nke mpaghara chromatin mepere emepe n'okpuru ọnọdụ oge-oge. ATAC-seq na-enye echiche zuru oke nke odida obodo chromatin nwere ike ịnweta, n'adịghị ka ụzọ na-elekwasị anya naanị na saịtị njide ihe ederede ma ọ bụ mpaghara akụkọ ihe mere eme gbanwere. Site n'ịhazi mpaghara chromatin ndị a mepere emepe, ATAC-seq na-ekpughe mpaghara o yikarịrị ka ọ ga-enwe usoro iwu na-arụ ọrụ yana saịtị nwere ike ijikọ ederede, na-enye nghọta bara uru na mgbanwe mgbanwe mkpụrụ ndụ ihe nketa n'ofe genome.
-
16S/18S/ITS Amplicon Sequencing-PacBio
Mkpụrụ ndụ ihe nketa 16S na 18S rRNA, yana mpaghara Internal Transcribed Spacer (ITS), na-eje ozi dị ka akara akara mkpịsị aka mkpụrụ ndụ ihe dị mkpa n'ihi nchikota ha nke mpaghara echekwara nke ukwuu yana hyper-agbanwe agbanwe, na-eme ka ha bụrụ ngwa bara uru maka ịkọwapụta ihe dị ndụ prokaryotic na eukaryotic. Mmụba na usoro nke mpaghara ndị a na-enye ụzọ na-enweghị ikewapụ maka nyocha ihe mejupụtara microbial na ụdị dị iche iche n'ofe gburugburu ebe obibi dị iche iche. Ọ bụ ezie na usoro Illumina na-elekwasịkarị anya mpaghara hypervariable dị mkpụmkpụ dị ka V3-V4 nke 16S na ITS1, egosipụtara na nkọwa taxonomic dị elu bụ nke a ga-enweta site n'ịhazi ogologo 16S, 18S na ITS. Ụzọ a zuru oke na-ebute pasentị dị elu nke usoro nkewa nke ọma, na-enweta ọkwa nke mkpebi na-agbatị ruo n'ichepụta ụdị. PacBio's Single-Molecule Real-Time (SMRT) usoro usoro ihe omume pụtara ìhè site n'inye ogologo ọgụgụ dị ogologo (HiFi) nke na-ekpuchi amplicon ogologo zuru oke, na-emegide izi ezi nke usoro Illumina. Ikike a na-enye ndị na-eme nchọpụta ohere inweta uru na-enweghị atụ - echiche panoramic nke ọdịdị mkpụrụ ndụ ihe nketa. Mkpuchi agbatịkwuru na-ebuli mkpebi a dị na nkọwapụta ụdịrị, ọkachasị n'ime obodo nje ma ọ bụ fungal, na-eme ka nghọta miri emi banyere mgbagwoju anya nke ụmụ nje.
-
16S/18S/ITS Amplicon Sequencing-NGS
Usoro Amplicon na teknụzụ Illumina, na-elekwasị anya kpọmkwem 16S, 18S, na akara mkpụrụ ndụ ihe nketa ITS, bụ usoro siri ike maka ikpughe phylogeny, taxonomy, na ụdị dị ukwuu n'ime obodo microbial. Usoro a gụnyere ịhazi mpaghara hypervariable nke akara mkpụrụ ndụ ihe nketa. Ewebata ya na mbụ dị ka akara mkpisiaka molekul siteAhụhụ et alna 1977, usoro a agbanweela profaịlụ microbiome site n'ime ka nyocha na-enweghị ikewapụ onwe ya. Site na usoro nke 16S (bacteria), 18S (fungi), na Internal Transcribed Spacer (ITS, fungi), ndị nchọpụta nwere ike ịchọpụta ọ bụghị naanị ụdị dị ukwuu kamakwa ndị na-adịghị ahụkebe na ndị a na-amaghị. N'ịbụ nke anakwere dị ka ngwa ọrụ dị mkpa, usoro amplicon abụrụla ihe enyemaka n'ịmata ihe dị iche iche microbial mejupụtara n'ofe gburugburu dị iche iche, gụnyere ọnụ mmadụ, eriri afọ, stool, na karịa.
-
Usoro njegharị nje na fungal dum Genome
Bacterial na fungal dum-genome re-sequencing project bụ ihe dị mkpa maka ịkwalite microbial genomics site n'ịkwalite mmecha na ntụnyere nke genome microbial. Nke a na-eme ka injinia sie ike, njikarịcha usoro mmepụta ihe, yana nyocha nke ụzọ metabolism nke abụọ. Ọzọkwa, usoro fungal na nje bacteria dị oke mkpa maka ịghọta mgbanwe gburugburu ebe obibi, na-eme ka nsogbu dị mma, na ikpughe mgbanwe mkpụrụ ndụ ihe nketa, na-enwe mmetụta sara mbara na nkà mmụta ọgwụ, ọrụ ugbo, na sayensị gburugburu ebe obibi.
-
PacBio-Uni ogologo 16S/18S/ITS Usoro Amplicon
Amplicon (16S / 18S / ITS) n'elu ikpo okwu na-mepụtara na afọ nke ahụmahụ na microbial diversity oru ngo analysis, nke nwere ọkọlọtọ isi analysis na ahaziri analysis: isi analysis na-ekpuchi mainstream analysis ọdịnaya nke ugbu a microbial nnyocha, na analysis ọdịnaya bụ ọgaranya na keukwu. na nsonaazụ nyocha gosipụtara n'ụdị akụkọ akụkọ; Ọdịnaya nke nyocha ahaziri iche dị iche iche. Enwere ike ịhọpụta ihe nlele ma nwee ike ịtọ ntọala n'ụzọ dị nro dị ka akụkọ nyocha na ebumnuche nyocha siri dị, iji ghọta ihe achọrọ ahaziri iche. Sistemụ arụmọrụ Windows, dị mfe na ngwa ngwa.
-
Transcriptome-ogologo zuru oke nke PacBio (anaghị ntụnye aka)
Inweta data sequencing nke Pacific Biosciences (PacBio) dị ka ntinye, ngwa a nwere ike ịchọpụta usoro transcript zuru oke (na-enweghị mgbakọ). Site na ịdepụta usoro ogologo zuru oke megide genome ntụaka, enwere ike ịhazi transcript site na mkpụrụ ndụ amaara ama, transcripts, mpaghara koodu, wdg. N'okwu a, enwere ike nweta njirimara ziri ezi nke ihe owuwu mRNA, dị ka splicing ọzọ, wdg. Nyocha ọnụ na NGS transcriptome sequencing data na-enyere nkọwa zuru ezu na nkọwa zuru oke na okwu na ọkwa transcript, nke na-erite uru dị ukwuu n'okwu dị iche iche na nyocha ọrụ.
-
Mbelata Nnọchiteanya Bisulfite Sequencing (RRBS)
Mbelata Nnọchiteanya Bisulfite Sequencing (RRBS) apụtala dị ka ihe dị ọnụ ahịa na-arụ ọrụ nke ọma na usoro dum Genome Bisulfite Sequencing (WGBS) na nyocha DNA methylation. Ọ bụ ezie na WGBS na-enye nghọta zuru oke site n'inyocha genome dum n'otu mkpebi ntọala, ọnụ ahịa ya dị elu nwere ike ịbụ ihe na-egbochi ya. RRBS na-ebelata ihe ịma aka a n'ụzọ dabara adaba site n'ịhọrọ nyocha akụkụ nke genome. Usoro a na-adabere na mmụba nke mpaghara agwaetiti CpG nwere ụbara site na mpịaji MspI sochiri nha nha nke 200-500/600 bps. N'ihi ya, ọ bụ naanị mpaghara dị nso na agwaetiti CpG ka a na-ahazi usoro, ebe ndị nwere agwaetiti CpG dị anya ka ewepụrụ na nyocha ahụ. Usoro a, jikọtara ya na usoro nke bisulfite, na-enye ohere maka nchọpụta dị elu nke DNA methylation, na usoro nhazi usoro, PE150, na-elekwasị anya kpọmkwem na njedebe nke ntinye karịa n'etiti, na-abawanye arụmọrụ nke profaịlụ methylation. RRBS bụ ngwá ọrụ bara uru nke na-enyere nyocha DNA methylation dị ọnụ ahịa aka na ọganihu ihe ọmụma nke usoro epigenetic.
-
Usoro prokaryotic RNA
Usoro RNA na-enye ike ịkọwapụta nkọwa nke ederede RNA niile n'ime sel n'okpuru ọnọdụ akọwapụtara. Teknụzụ ọgbara ọhụrụ a na-arụ ọrụ dị ka ngwa ọrụ siri ike, na-ekpughe profaịlụ mkpụrụ ndụ dị mgbagwoju anya, usoro mkpụrụ ndụ ihe nketa, na usoro mkpụrụ ndụ jikọtara ya na usoro ndu dị iche iche. N'ịbụ nke anakwere na nyocha bụ isi, nchọpụta ụlọ ọgwụ, na mmepe ọgwụ, usoro RNA na-enye nghọta n'ime mgbagwoju anya nke ike ndụ cell na ụkpụrụ mkpụrụ ndụ ihe nketa. A haziri nhazi nlele prokaryotic RNA anyị maka transcriptomes prokaryotic, gụnyere mbelata rRNA na nkwadebe ọbá akwụkwọ ntụzịaka.
Platform: Illumina NovaSeq
-
Usoro Metatranscriptome
Iji teknụzụ usoro usoro Illumina, BMKGENE's metatranscriptome sequencing ọrụ na-ekpughere mkpụrụ ndụ ike dị ike nke ụdị dị iche iche nke ụmụ nje, na-agbasa eukaryotes na prokaryotes na nje, n'ime gburugburu eke dị ka ala, mmiri, oké osimiri, stool, na eriri afọ. Ọrụ zuru oke anyị na-enye ndị nyocha ike ịbanye n'ime profaịlụ okwu mkpụrụ ndụ ihe nketa zuru oke nke obodo microbial. Na agafe nyocha taxonomic, ọrụ usoro usoro metatranscriptome anyị na-eme ka nyocha n'ime ịba ụba ọrụ, na-eme ka ìhè dị na mkpụrụ ndụ ihe nketa egosipụtara dị iche iche na ọrụ ha. Choputa otutu ihe nleba anya nke ndu ka ị na-agagharị n'ọdịdị mgbagwoju anya nke okwu mkpụrụ ndụ ihe nketa, ụdị taxonomic dị iche iche, na arụrụ ọrụ n'ime niche gburugburu ebe obibi ndị a dị iche iche.
-
De novo Fungal Genome Assembly
BMKGENE na-enye azịza dị iche iche maka mkpụrụ ndụ fungal, na-egbo mkpa nyocha dị iche iche yana izu oke genome chọrọ. Iji usoro Illumina agụ nkenke naanị na-enye ohere ịmepụta mkpụrụ ndụ ihe nketa. A na-ejikọta usoro ọgụgụ dị mkpirikpi na usoro ọgụgụ ogologo oge site na iji Nanopore ma ọ bụ Pacbio maka genome fungal a nụchara anụcha nke nwere ogologo contigs. Ọzọkwa, ijikọ usoro Hi-C na-eme ka ikike ahụ dịkwuo elu, na-enye ohere ịnweta genome-ọkwa chromosome zuru oke.
