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BMKMANU S3000_Spatial transcriptome
transcriptomics spatial na-eguzo n'ihu nke mmepụta sayensị, na-enye ndị nchọpụta ike ịbanye n'ime usoro okwu mkpụrụ ndụ mgbagwoju anya n'ime anụ ahụ ma na-echekwa ọnọdụ ha. N'etiti nyiwe dị iche iche, BMKGene ewepụtala BMKManu S3000 Spatial Transcriptome Chip, na-anya isi mkpebi siri ike nke 3.5µm, na-eru nso nso cell cell, na-enyere ntọala mkpebi ọkwa dị iche iche. Ihe mgbawa S3000, nke na-egosipụta ihe dị ka nde ntụpọ anọ, na-arụ ọrụ microwells nke nwere beads juru na nyocha njide kpuchiri oghere. A na-akwado ọba akwụkwọ cDNA, nke ejiri koodu barcode mejuo ya, site na mgbawa S3000 wee mee ya n'usoro n'elu ikpo okwu Illumina NovaSeq. Nchikota nke ihe nlele oghere na UMI na-eme ka o doo anya na izi ezi na nkọwapụta nke data ewepụtara. Ihe mgbawa BMKManu S3000 na-agbanwe agbanwe nke ukwuu, na-enye ntọala mkpebi ọkwa dị iche iche nke enwere ike ịhazi ya nke ọma na anụ ahụ dị iche iche yana ọkwa nkọwa achọrọ. Ngbanwe a na-edobe mgbawa ahụ dị ka nhọrọ pụtara ìhè maka ọmụmụ ihe gbasara transcriptomics dị iche iche, na-agba mbọ hụ na mkpokọta oghere nwere obere mkpọtụ. Ojiji teknụzụ nkewa cell na BMKManu S3000 na-enyere aka ikewapụta data ederede na ókèala nke mkpụrụ ndụ, na-ebute nyocha nke nwere nkọwa ndu kpọmkwem. Ọzọkwa, nkwubi okwu nke S3000 emelitere na-ebute ọnụ ọgụgụ dị elu nke mkpụrụ ndụ ihe nketa na UMI nke achọpụtara n'otu sel, na-eme ka nyocha nke ọma karịa nke usoro ntụgharị ohere na nchịkọta mkpụrụ ndụ.
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DNBSEQ ụlọ akwụkwọ emeburu
DNBSEQ, nke MGI mebere, bụ teknụzụ NGS ọhụrụ nke jisiri ike belata n'ihu ọnụ ahịa usoro na ịbawanye mmepụta. Nkwadebe nke ọba akwụkwọ DNBSEQ gụnyere nkewa DNA, nkwadebe nke ssDNA, na mmụba okirikiri iji nweta DNA nanoballs (DNB). A na-ebunye ihe ndị a n'elu elu siri ike ma mechaa soro ya site na ngwakọta Probe-Anchor Synthesis (cPAS). Teknụzụ DNBSEQ na-ejikọta uru dị n'inwe mpe mpe mpe mpe mpe mpe akwa yana iji ụkpụrụ njehie njupụta dị elu na nanoballs, na-ebute usoro na ntinye dị elu na izi ezi.
Ọrụ usoro ọba akwụkwọ anyị emeburu na-enyere ndị ahịa aka ịkwadebe ọba akwụkwọ nke Illumina sitere na isi mmalite dị iche iche (mRNA, genome dum, amplicon, ọba akwụkwọ 10x, n'etiti ndị ọzọ), nke agbanwere na ọba akwụkwọ MGI n'ime ụlọ nyocha anyị ka edobere ya na DNBSEQ-T7, na-enyere aka. nnukwu data ego na ọnụ ala.
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Mmekọrịta Chromatin dabere na Hi-C
Hi-C bụ usoro emebere iji weghara nhazi genomic site na ijikọta mmekọrịta dabere na nso nso yana usoro ntinye dị elu. Usoro a dabere na chromatin crosslinking na formaldehyde, na-esote mgbaze na re-ligation n'ụzọ na ọ bụ naanị iberibe ihe jikọrọ ọnụ ga-emepụta ngwaahịa ligation. Site n'usoro ngwaahịa ligation ndị a, ọ ga-ekwe omume ịmụ nhazi 3D nke genome. Hi-C na-enyere aka n'ịmụ nkesa nke akụkụ nke genome nke a na-anakọta nke ọma (A compartments, euchromatin) na o yikarịrị ka ọ ga-arụ ọrụ transcriptionally, na mpaghara ndị a na-akpachi anya (akụkụ B, Heterochromatin). A pụkwara iji Hi-C mee ka ọ pụta ìhè Topologically Associated Domains (TADs), mpaghara nke genome nke nwere ihe owuwu apịaji ma nwee ike inwe usoro okwu yiri ya, na ịchọpụta loops chromatin, mpaghara DNA nke ndị na-edozi jikọtara ọnụ na nke ahụ bụ. na-emekarị ọgaranya na ihe nchịkwa. Ọrụ usoro Hi-C nke BMKGene na-enye ndị na-eme nchọpụta ike inyocha akụkụ mbara igwe nke genomics, na-emepe ụzọ ọhụrụ maka ịghọta ụkpụrụ genome na ihe ọ pụtara na ahụike na ọrịa.
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TGuide Smart Igwe Ọdụdọ Igwe Ọdụdọ RNA
TGuide Smart Igwe Ọdụdọ Igwe Ọdụdọ RNA
Sachaa mkpokọta RNA dị elu site na anụ ahụ osisi
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TGuide Smart Blood/Sell/Tissue RNA Kit
TGuide Smart Blood/Sell/Tissue RNA Kit
The prefilled cartridge / efere reagent kit maka ịdị ọcha nke mkpụrụ dị elu, ịdị ọcha dị elu, ịdị mma dị elu, mkpokọta RNA na-enweghị ihe mgbochi sitere na anụ ahụ / cell / ọbara zuru oke.
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TGuide Smart Igwe Ọdụdọ Igwe DNA
TGuide Smart Igwe Ọdụdọ Igwe DNA
Sachapụ DNA genomic dị elu site na anụ ahụ osisi dị iche iche
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Ngwa TGuide Smart Ala / Stool DNA Kit
Ngwa TGuide Smart Ala / Stool DNA Kit
Na-asachapụ DNA na-enweghị ihe mgbochi nke ịdị ọcha dị elu na ịdị mma site na nlele ala na stool
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Kit DNA dị ọcha TGuide
Na-eweghachite DNA dị elu site na ngwaahịa PCR ma ọ bụ gels agarose.
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TGuide Smart Blood Genomic DNA Kit
TGuide Smart Blood Genomic DNA Kit
Ngwa nke katrij / efere reagent nke prejuputara maka ịsacha DNA nke mkpụrụ ndụ ihe nketa site na ọbara na akwa mkpuchi.
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TGuide Smart Magnetic Tissue DNA Kit
Ngwa ihe reagent nke katrij / efere emejuru nke ọma maka mmịpụta DNA genomic site na anụ ahụ anụmanụ
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TGuide Smart Universal DNA Kit
Ngwa nke katrij / efere reagent prefilled maka ịdị ọcha genomic DNA site na ọbara, ntụpọ ọbara akpọnwụwo, nje bacteria, sel, asu, swabs ọnụ, anụ ahụ anụmanụ, wdg.
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TGuide S16 Nucleic Acid Extractor
TGuide S16 Nucleic Acid Extractor
Ngwa Benchtop dị mfe iji, nlele 1-8 ma ọ bụ 16 n'otu oge.
Nọmba katalọgụ / nkwakọ ngwaahịa
Nwamba. mba
ID
Mba nke preps
OSE-S16-AM
1 set
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Ngwọta mRNA PacBio 2+3 zuru oke
Ọ bụ ezie na usoro mRNA dabeere na NGS bụ ngwá ọrụ dị iche iche maka ịkọwapụta mkpụrụ ndụ ihe nketa, ịdabere na mkpirisi agụ na-egbochi ịdị irè ya na nyocha transcript dị mgbagwoju anya. N'aka nke ọzọ, PacBio sequencing (Iso-Seq) na-eji teknụzụ na-agụ ogologo oge, na-eme ka usoro nke transcript mRNA zuru oke. Usoro a na-eme ka nyocha zuru oke nke splicing ọzọ, njikọ mkpụrụ ndụ ihe nketa, na poly-adenylation, n'agbanyeghị na ọ bụghị nhọrọ bụ isi maka ịkọwapụta mkpụrụ ndụ ihe nketa. Ngwakọta 2 + 3 na-ejikọta ọdịiche dị n'etiti Illumina na PacBio site na ịdabere na PacBio HiFi na-agụ iji chọpụta nhazi nke transcript nke isoforms na usoro NGS iji chọpụta ihe ndị yiri ya.
Platforms: PacBio Sequel II/ PacBio Revio na Illumina NovaSeq;
