Ndekọ ederede Pacbio zuru oke

PacBio Full-Length Transcriptome Sequencing, Isoseq, na-eme ka njirimara ziri ezi nke transcript isoforms, na-eme ka ìhè dị na polyadenylation ọzọ na splicing, na-eduga na nyocha mkpụrụ ndụ ihe nketa ziri ezi. Emebere pipeline transcriptome ogologo-ogologo BMKCloud PacBio iji nyochaa ọba akwụkwọ cDNA ndị e debere n'usoro usoro nkwekọrịta okirikiri (CCS) wee chọpụta usoro nke na-abụghị nke chimeric (FLNC) zuru oke, bụ nke a na-agbakọta n'ime ndegharị na-abụghị nke anaghị arụ ọrụ. Nyocha BUSCO na-esote na-enyocha izu oke nke mgbakọ transcriptome. Site na transcriptome a gbakọtara, a na-eme nyocha dị iche iche: splicing ọzọ, usoro dị mfe ikwugharị (SSR), amụma nke lncRNA na mkpụrụ ndụ ihe nketa kwekọrọ, amụma mkpụrụ ndụ ihe nketa, nyocha mkpụrụ ndụ ihe nketa ezinụlọ, nyocha ihe odide ederede, na nkọwa ọrụ nke transcripts.