MrNA-SEQ (NGS) na Perpame

MrNA-SEQ (NGS) na Perpame

RNA-SEQ bu ihe ọkọlọtọ ngwa ahịa n'ofe nke ndụ na sayensị mkpụrụ osisi, na-egbochi oghere dị n'etiti mkpụrụ ndụ mkpụrụ ndụ na protes. Ike ya dabere n'ịchọpụta transcript ọhụrụ ma kpalie okwu ha n'otu n'otu n'otu. A na-ejikarị ya ọmụmụ ihe nke mkpụrụ ndụ ihe nketa ma ọ bụ phenotypes dị iche iche ma ọ bụ na-ekpughe ọdịdị nke mkpụrụokwu n'okpuru ọnọdụ. Ngwaahịa BMNA (nyocha) na-eme nyocha, nyocha dị iche iche (deg), na ndị na-eme nyocha na-eme ka ọ dị na MRA-SOO-SOO-SOON (NGS) na-enwe ike na-eme ụdị ngwanrọ yiri ya. Ndị ọrụ nwere ike bulite data ha Rna-seq na igwe ojii, ebe ngwa ahụ na-enye usoro nyocha Bioininformatic. Ọzọkwa, ọ na-ebute ahụmịhe ndị ahịa, na-enye ndị ọrụ ahaziri ahazi na mkpa ndị ọrụ. Ndị ọrụ nwere ike ịtọ parameters ma nyefee ozi Pipeline ma nyefee ozi ọkpọkọ, lelee data mmekọrịta, na eserese data, ịmịnye ụyọkọ, matg.

Nsonaazụ ngosi

Ngwuputa data

Bubata A Ga-achọ

Nnyocha ndị

Nrutuaka

Nsonaazụ ngosi

Ngwuputa data

Bubata A Ga-achọ

Uzuoku:Wind Ward, MGI
Atụmatụ:Rna-seq
Plaanu obodo: Tụnyere, dị ọcha data.
Typedị Ọba:fr-frststrand, fr-offstrand ma ọ bụ fr-nke abụọ
Gụọ ogologo:150 BP
Filedị faịlụ:* .faliv, * .fq, * Usoro ahụ ga-emena-akpaghị aka.EG * _1.avelkq na-ejikọtara * ._ 2.fafa.
Onu ogugu:Enweghị mgbochi na ọnụọgụnke eserese, mana oge nyocha ga-abawanye ka ọnụ ọgụgụ nkeihe nlele toro.
Ego a tụrụ aro:6g kwa ihe nlele

Nnyocha ndị

Ngwá nyocha ahụ na ngwa ahịa nke mrna-seq (ntụaka)Pipeline dị ka ndị a:

1. RAWDATA njikwa:

• Iwepụ usoro dị ọnụ ala, ihe nkwụnye ego,wdg;

• Ngwaọrụ: n'ime-ulo etoliteworo pipeline;

• Ikwekọ na-agụ akwụkwọ mara mmakpọtụrụ Genome.

• Ngwaọrụ:Itathat2, samtools

3. Nyochaa Ọma:

• Tinye ogologo oge, nyocha nke usoro ọmụmụ, wdg;

• Ngwaọrụ:samtools;

4. Nyochaa usoro nhazi:

Nyochaa nyocha na-acha uhie uhie, na njikarịcha okike,Amụma edinata akwụkwọ ọgụgụ ahụ, wdg;

• Ngwaọrụ:Strgtie, gFfmar, Gaket,Dayamondu, Infrarproscan, naHmmer.

• Nnyocha nyocha, nyocha mmekọrịta, ọrụọburù;

•Nsonaazụ ọhụụ;

•RnaSgseq, Deseq2, ggplot2, Dexseq

Nrutuaka

1. Kim, Dahwan et al. "Inye aha ihe osise sitere na eserese naGenotyping na Herat2 na Herat-Chọọgburu. "AgwaBiotechnology37 (2019): 907 - 915.

2. McKenna, Eron Et al. "Ntụle nyocha nke mkpụrụ ndụ"Usoro eji eme ya maka nyocha ọzọdata data. "Nnyocha okike20 9 (2010): 1297-303.

3. Li, Hen Et al. "Usoro nhazi / Map naSamtools. "Bioniekatics25 (2009): 2078 - 2079.

4. Perţea, Mihaela Et al. "Stgetie entertieNtughari nke transcrictome si rna-seq na-agụ. "AgwaBiotechnology33 (2015): 290-295.

5. Lovehụnanya, Michael I. et al. "Atụmatụ na-agbanwe agbanweMgbasa maka data Rna-SEQ. "KeenniiBayoloji15 (2014): n. ọ na-akụ.

6. EDDY, Sean r .. "Agwuwo ngwa ngwa profaịlụ HMM."Ploso Compational bayotional7 (2011): n. ọ na-akụ.

MrNA-SEQ (NGS) na Perpame

MrNA-SEQ (NGS) na Perpame

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