Usoro transcriptome dabere Nanopore zuru oke
Usoro Nanopore na-ekewa onwe ya na usoro nhazi usoro ndị ọzọ, n'ihi na a na-agụ nucleotides ozugbo na-enweghị njikọ DNA ma na-emepụta ogologo oge na iri puku kilobases. Nke a na-enye ike ịgụpụta ndegharị n'ogologo zuru oke yana imeri ihe ịma aka ndị dị na ọmụmụ transcript.
√ Ọdịiche dị n'usoro dị ala
√ Ngụpụta cDNA zuru oke maka ọmụmụ nhazi usoro mkpụrụ ndụ ihe nketa
√ Obere data achọrọ iji kpuchie otu ọnụọgụ ederede
√ Ịmata ọtụtụ isoforms kwa mkpụrụ ndụ ihe nketa
√ Nkọwapụta okwu na ọkwa isoform
Pipeline Bioinformatics a na-ahụkarị
Okwu na-aga nke ọma kacha ọhụrụ na Teknụzụ Biomarker
1. Ntụle ntule nke transcriptomes zuru oke na-ekpughe Gnetum luofuense stem development dynamics.
Akwụkwọ akụkọ: Frontiers na Genetics
Ebipụtara: Maachị 2021
isiokwu: MinION | Nhọrọ ọzọ splicing | APA | lncRNA | Ederede ederede dị iche iche
Gụọ Full-ederede
2. Ntugharị Unidirectional nke obere RNA site na ome gaa na mgbọrọgwụ na heterografts interspecific.
Akwụkwọ akụkọ: Osisi okike
Ebipụtara: Jenụwarị 2021
isiokwu: Nanopore | Illumina | nchọpụta mRNA mkpanaka mkpanaka ogologo zuru oke
3. Ntụle transcriptome zuru oke nke mgbọrọgwụ asparagus na-ekpughe usoro mkpụrụ ndụ nke nnabata nnu nke arbuscular mycorrhizal fungi kpatara.
Akwụkwọ akụkọ: Environmental and Experimental Botany
Ebipụtara: Jenụwarị 2021
isiokwu: MinION | Edemede egosipụtara dị iche iche | Ihe odide ederede | Nhọrọ ọzọ splicing
4. Mgbanwe njirimara anụ ahụ na ntụgharị zuru oke nke Rose (Rosa chinensis) Mgbọrọgwụ na akwụkwọ na-azaghachi maka nrụgide oké ọkọchị.
Akwụkwọ akụkọ: Physiology Plant and Cell
Ebipụtara: Ọktoba 2020
isiokwu: PromethION | Edemede egosipụtara dị iche iche | Amụma na-arụ ọrụ | Isoforms gbawara agbawa | TF na lncRNA
5. Nyocha na ntụle zuru oke nke PacBio na usoro RNA dabeere na nanopore nke transcriptome Arabidopsis.
Akwụkwọ akụkọ: Ụzọ osisi
Ebipụtara: Eprel 2020
isiokwu: GridION | PromethION | PacBio Sequel | Illumina NovaSeq | Nhazi genome | njirimara transcript | Nhọrọ ọzọ splicing | SSR | LncRNA | Isoform quantification
Akụkọ na isi ihe Ebumnuche iji Biomarker Teknụzụ kekọrịta ikpe kachasị ọhụrụ na-aga nke ọma, na-ewepụta mmezu sayensị ọhụụ yana usoro ama ama etinyere n'oge ọmụmụ ihe.
Oge nzipu: Jan-08-2022