TGuide Smart Igwe Ọdụdọ Igwe Ọdụdọ RNA
Nọmba katalọgụ / nkwakọ ngwaahịa
| Nwamba. mba | ID | Mba nke preps |
| 4993552 (katrij) | DP662-DE | 48 |
Ntuziaka nhọrọ reagent maka TGuide S16
| Nuclei acid | Ụdị Nlereanya | Aha ngwaahịa | Nha mbukota (preps) Katrij / efere | Nwamba mba. Katrij / efere |
| DNA | Anụ anụ | TGuide Smart Magnetic Tissue DNA Kit | 48/96 | 4993547/4995038 |
| DNA | Osisi & Mkpụrụ | TGuide Smart Igwe Ọdụdọ Igwe DNA | 48 | 4993548 |
| DNA | Ala/Stool | TGuide Smart Ala / Ngwa DNA Stool | 48 | 4993549 |
| DNA | Gel & PCR ngwaahịa | Kit DNA dị ọcha TGuide | 48 | 4993550 |
| DNA | Ọbara dum | TGuide Smart Blood Genomic DNA Kit | 48/96 | 4993703/4995206 |
| DNA | Cell/Swab/Ebe akọrọ, wdg | TGuide Smart Universal DNA Kit | 48/96 | 4993704/4995040 |
| RNA | Ọbara/Mkpụrụ ndụ/anụ ahụ | TGuide Smart Blood/Sell/Tissue RNA Kit | 48/96 | 4993551/4995043 |
| RNA | Osisi & Mkpụrụ | TGuide Smart Igwe Ọdụdọ Igwe Ọdụdọ RNA | 48 | 4993552 |
| DNA/RNA | Fluids efu (Serum, wdg) | TGuide Smart Viral DNA/RNA Kit | 48/96 | 4993702/4995207 |
Ntinye ngwaahịa
Sachapụ mkpụrụ ndụ dị elu, ịdị ọcha dị elu, mkpokọta RNA dị elu site na ọbara/sel/anụ ahụ.
Enweghị reagents na-egbu egbu dị ka phenol/chloroform nwere
RNA enwetara nwere ịdị ọcha dị elu ma enwere ike iji ya ozugbo maka nchọpụta mgbawa, usoro ntinye dị elu na nnwale ndị ọzọ.
Atụmatụ
Dị mfe iji
Enweghị ọrụ pipụ ọzọ.
Enwere ike gbanwee olu elution dị ka achọrọ.
Nwụnye na ọrụ chọrọ obere ọzụzụ. Site na mmemme etinyegoro mbụ, bupụ katrijị ahụ, họrọ protocol wee mee nnwale gị. Mmemme dị njikere iji, yana enwere ike ịhazi ya.
Ebugoro reagents yana ngwa mkpofu dabara adaba.
A na-ekpochapụ njikwa kemịkalụ ruo oke kachasị iji hụ na nchekwa ọrụ ahụ.
Nsonaazụ ngosi
Mkpokọta mgbọrọgwụ RNA ọka wheat
Nlele nha: 100 mg
Sample pretreatment: egweri na mmiri mmiri nitrogen ma ọ bụ obere okpomọkụ anụ ahụ homogenizer ọgwụgwọ
Ntinye uche Agarose: 2% (TAE)
Ibu ibu: 2 μl
Akara: Akara IV, TIANGEN
Nsonaazụ nnwale: iji TGuide S16 enwetara na reagent 4993552 iji wepụta mkpokọta RNA nke mgbọrọgwụ ọka na-akpaghị aka nwere mkpụrụ dị mma na ịdị ọcha dị elu. N'ime nnwale a, ewepụtara ihe dị ka 20 μg nke nucleic acid site na mgbọrọgwụ 100 mg nke ọka wit, yana OD260 / OD280 gburugburu 2.0 ~ 2.1, na OD260 / OD230 ~ 2.0.
Mkpokọta mmịpụta RNA sitere na nlele osisi dị iche iche
Nlele nha: 100 mg
Nlereanya pretreatment: ala-okpomọkụ homogenizer
Agarose gel ntinye uche: 1% (TAE)
Ibu ibu: 1 μl
M: Marker III, TIANGEN
1-4: mkpụrụ ọka 5-8: akwụkwọ ọka 9-12: mgbọrọgwụ kabeeji 13-16: agwa soy
Ewepụtara ihe nlele abụọ mbụ site na ngwa nwepụta nke kọlụm ma wepụta sample abụọ ikpeazụ site na TGuide S16.
Nsonaazụ nnwale: mpụta mmịpụta nke osisi RNA site na TGuide S16 nwere reagent 4993552 dabara ma ọ bụ dị mma karịa nke ahụ site na ngwa mmịpụta ogho. Ịdị ọcha na iguzosi ike n'ezi ihe bụ otu ihe ahụ. Ya mere, enwere ike iji TGuide S16 wepụta mkpokọta RNA osisi iji dochie ngwọta mmịpụta dabere na kọlụm.
