TGuide smart / sel / anụ ahụ rna Kit
Nọmba katalọgụ / nkwakọ ngwaahịa
| Pusi. mba | ID | Mba nke preps |
| 4995043 (efere) | Dp661-e | 96 |
| 499351111 (katride) | Dp661-de | 48 |
Ntuziaka reagent maka TGuide S16
| Nuclei acid | Ụdị atụ | Aha | Ogo ngwongwo (preps) Katriki / efere | Pusi no. Katride / efere |
| Dina | Anụ ahụ | TGuide Smart Smart Health Dna Kit | 48/96 | 4993547/4995038 |
| Dina | Osisi & Mfri | TGuide Smart Smartic osisi DNA KIT | 48 | 4993548 |
| Dina | Ala / stool | TGuide Smart Smart / Stool DNA KIT | 48 | 4993549 |
| Dina | Gel & Pcr Ngwaahịa | TGuide Smart DNA | 48 | 4993550 |
| Dina | Ọbara niile | TGuide smart ọbara gna Kit | 48/96 | 4993703/499520202020206 |
| Dina | Sel sel / swab / akọrọ, wdg | TGuide Smart Universal Dna Kit | 48/96 | 4993704/4995040 |
| Rona | Ọbara / sel / anụ ahụ | TGuide smart / sel / anụ ahụ rna Kit | 48/96 | 4993551/49543 |
| Rona | Osisi & Mfri | TGuide Smart Smartic osisi RNA Kit | 48 | 4993552 |
| DNA / RNA | Cell free (Ser, wdg) | TGuide Smart DNA / RNA KIT | 48/96 | 4993702/49952020207 |
Nmkpọ Okwu mmalite
Acha ọcha - mkpụrụ, ịdị ọcha, ngụkọta dị elu nke ọbara / akwara.
Ọ dịghị abụ ndị na-egbu egbu dị ka phenol / chloroform dị ka
RNA nwetara nwere ịdị ọcha dị elu ma enwere ike iji ya maka nchọpụta mgbawa, ụzọ mgbapụ elu na ihe ndị ọzọ dị elu.
EGO
Super mfe iji
Ọ dịghị ọrụ ọkpọkọ.
Enwere ike idozi olu ịlegharị anya dịka achọrọ.
Nwụnye na ọrụ chọrọ obere ọzụzụ. Site na mmemme awụnyere tupu awụnyere ya, wezụga ngwa azụ, họrọ ogbugba ndu ma na-agba ọsọ. Mmemme dị njikere iji ya, yanakwa ọdịiche.
Ndị na-ede ego na-ere ahịa ma kwekọọ.
A na-ewepụkwa njikwa kemịkalụ ruo na nchekwa kachasị mma.
Nsonaazụ ngosipụta
A na-eji ihe iri abụọ na abụọ na iri abụọ na asaa.
Isi iyi nke: CD-1 Nice
Ọnọdụ nlele: ọbara zuru oke
Ogo nha: 200 μl
Nsonaazụ: Nchịkọta RNA wepụtara site na ọbara site na TGuide S16 kwadebere 4993551 nwere ezigbo mkpụrụ na ịdị ọcha nke ala
uzo uzo di elu.
Isi iyi nke: CD-1 Nice
Olu na-eburu: 1 μl. Emetụta imeju ahụ ugboro ise ma buru ibu.
Agarose gel ịta: 1%. Electrophoresis maka 20 min na 6 v / cm;
Nna-ukwu Tiangen Marter III 1-4: obi 5-8:
Imeju 9-12: Ngụgụ 13-16: akụrụ
Ihe nlere abuo nke nke aru nke ọ bụla site na mkpụrụ osisi na-esite na ngwa, na nke ikpeazụ na nke ikpeazụ wepụtara na TGuide S16 na akpaghị aka site na TGuide S16.
NSOGBU NIILE: Nchịkọta anụ ahụ na-akpaghị aka site na TGuide S16 kwadebere 4993551 nwere nnukwu mkpụrụ, nke ya na RNA wepụtara ngbanwe sitere na mmịpụta. Ihe dị ọcha bụ otu, ya na OD260 / OD280 dị gburugburu 2.0 ~ 2.1 na OD260 / OD230> 2.0. Na mmechi, enwere ike iji TGuide S16 wepu ngụkọta RNA site na anụ ahụ na nnọchi nke mkpụrụ osisi na-agbanwe agbanwe.
