TGuide Smart Igwe Ọdụdọ Igwe DNA
Nọmba katalọgụ / nkwakọ ngwaahịa
| Nwamba. mba | ID | Mba nke preps |
| 4993548 (katrij) | DP607-DE | 48 |
Ntuziaka nhọrọ reagent maka TGuide S16
| Nuclei acid | Ụdị Nlereanya | Aha ngwaahịa | Nha mbukota (preps) Katrij / efere | Nwamba mba. Katrij / efere |
| DNA | Anụ anụ | TGuide Smart Magnetic Tissue DNA Kit | 48/96 | 4993547/4995038 |
| DNA | Osisi & Mkpụrụ | TGuide Smart Igwe Ọdụdọ Igwe DNA | 48 | 4993548 |
| DNA | Ala/Stool | TGuide Smart Ala / Ngwa DNA Stool | 48 | 4993549 |
| DNA | Gel & PCR ngwaahịa | Kit DNA dị ọcha TGuide | 48 | 4993550 |
| DNA | Ọbara dum | TGuide Smart Blood Genomic DNA Kit | 48/96 | 4993703/4995206 |
| DNA | Cell/Swab/Ebe akọrọ, wdg | TGuide Smart Universal DNA Kit | 48/96 | 4993704/4995040 |
| RNA | Ọbara/Mkpụrụ ndụ/anụ ahụ | TGuide Smart Blood/Sell/Tissue RNA Kit | 48/96 | 4993551/4995043 |
| RNA | Osisi & Mkpụrụ | TGuide Smart Igwe Ọdụdọ Igwe Ọdụdọ RNA | 48 | 4993552 |
| DNA/RNA | Fluids efu (Serum, wdg) | TGuide Smart Viral DNA/RNA Kit | 48/96 | 4993702/4995207 |
Ntinye ngwaahịa
Ọ dabara nke ọma: Ọ dabara maka ụdị anụ ahụ dị iche iche, ọkachasị osisi polysaccharide ma ọ bụ polyphenol bara ụba.
Nchekwa na nke anaghị egbu egbu: Ọ nweghị reagents organic na-egbu egbu dị ka phenol/chloroform nwere.
Ịdị ọcha dị elu: DNA enwetara nwere ịdị ọcha dị elu ma nwee ike iji ya mee ihe ozugbo maka nchọpụta mgbawa, usoro ntinye dị elu na nyocha ndị ọzọ.
Atụmatụ
Dị mfe iji
Enweghị ọrụ pipụ ọzọ.
Enwere ike gbanwee olu elution dị ka achọrọ.
Nwụnye na ọrụ chọrọ obere ọzụzụ. Site na mmemme etinyegoro mbụ, bupụ katrijị ahụ, họrọ protocol wee mee nnwale gị. Mmemme dị njikere iji, yana enwere ike ịhazi ya.
Ebugoro reagents yana ngwa mkpofu dabara adaba.
A na-ekpochapụ njikwa kemịkalụ ruo oke kachasị iji hụ na nchekwa ọrụ ahụ.
Nsonaazụ ngosi
Genomic DNA mmịpụta nduku
Nlele nha: 100 mg
Sample tupu ọgwụgwọ: egweri na mmiri mmiri nitrogen ma ọ bụ anụ ahụ homogenizer
Agarose gel ntinye uche: 2% (TAE)
Ibu ibu: 2 μl
Ihe nrịbama: D15000, TIANGEN S16-01, S16-02 na S16-03 na-anọchi anya nnwale ndị yiri nke ịwepụta DNA genomic osisi site na iji 3 TGuide S16 Nucleic Acid Extractors n'otu oge.
Ụlọ ọrụ: akara ama ama
Nsonaazụ nnwale: iji TGuide S16 na reagent 4993548 wepụta DNA mkpụrụ ndụ ihe nketa na-akpaghị aka, enwetala mkpụrụ dị mma na ịdị ọcha dị elu, nke dabara na njiri ngwaahịa mmịpụta nke ngwa nbipụta nke ama ama. N'ime nnwale a, a na-ewepụta ihe dị ka 11 μg nke nucleic acid site na 100 mg nduku buds, yana OD260 / OD280 gburugburu na 1.8 ~ 1.9, na OD260 / OD230 -2.0.
Mwepụta DNA nke mkpụrụ osisi soybean
Nlele nha: 100 mg
Sample tupu ọgwụgwọ: egweri na mmiri mmiri nitrogen ma ọ bụ anụ ahụ homogenizer
Agarose gel ntinye uche: 1% (TAE)
Ibu ibu: 2 μl
Akara: D15000, TIANGEN
Nsonaazụ nnwale: iji TGuide S16 nwere reagent 4993548 wepụta DNA genomic nke akwụkwọ soybe na-akpaghị aka enwetala mkpụrụ dị mma na ịdị ọcha dị elu. N'ime nnwale a, ewepụtara ihe dị ka 13μg nke nucleic acid site na 100 mg soybean epupụta, yana OD260/OD280 gburugburu 1.8 ~ 1.9, na OD260/OD230 -2.0.
Genomic DNA mmịpụta nke mgbọrọgwụ ọka wit
Nlele nha: 100 mg
Sample tupu ọgwụgwọ: egweri na mmiri mmiri nitrogen ma ọ bụ anụ egweri homogenizer
Agarose gel ntinye uche: 1% (TAE)
Ibu ibu: 2 μl
Akara: D15000, TIANGEN
Ụlọ ọrụ: akara ama ama
Nsonaazụ nnwale: iji TGuide S16 na reagent 4993548 wepụta DNA genomic nke mgbọrọgwụ ọka , enwetala mkpụrụ dị mma na ịdị ọcha dị elu, nke ya na mpụta mmịpụta na ịdị ọcha nke ndị asọmpi ama ama. Ọzọkwa, iguzosi ike n'ezi ihe nke DNA genomic dị mma karịa ngwaahịa asọmpi nke ụlọ ọrụ ndị ọzọ. N'ime nnwale a, ewepụtara ihe dị ka 15 μg nke nucleic acid site na mgbọrọgwụ 100 mg nke ọka wit, yana OD260 / OD280 gburugburu 1.8 ~ 1.9, na OD260 / OD230 ~ 2.0.
