Ogologo ORNA ROAQUENT -PACKO
EGO
● Sinnesis sitere na Poly-Arna sochiri nkwadebe ọbá akwụkwọ
● Sequencing na ọnọdụ CCS, na-ekepụta HIFI na-agụ
● Seaquing nke transcript zuru oke
Nnyocha a apụtaghị onye Keynome; Agbanyeghị, enwere ike iji ya rụọ ọrụ
Nchịkọta Bioinmatic Na-eme nyocha nke transcripts Iscript Lncrim, Gene nduru, poly-adlylition, na usoro ọmụmụ
Uru bara uru
●Omume: Hifi na-agụ nke ziri ezi> 99.9% (Q30), na NGS
● Nmapụta na-acha uhie uhie: Sequencen nke transcript niile na-eme ka njiri mara akpa na njirimara
●Nnukwu nkasi ike: Site na ihe ndekọ egwu nke pacbio nke 1100 rụchara ọrụ zuru oke ma na-ahazi ihe karịrị 2300, ndị otu anyị na-eweta ọtụtụ ahụmịhe maka ọrụ niile.
●Nkwado Azụmaahịa: Nrara anyị na-agbasa karịrị ọrụ ngwụcha ọnwa atọ. N'oge a, anyị na-enye enyemaka na-esochi, enyemaka nsogbu, na Q & A na-ekwu maka ajụjụ ọ bụla metụtara nsonaazụ ya.
Nlele Nlere Anya na Nnyefe
| Oba akwukwo | Atụmatụ sequencing | Data tụrụ aro | Njikwa Dị Mma |
| Polya E Nwere Onyinye MRA CCS CCS | Pacbio sequel II Pacbio revo | 20/40 GB 5/10 m ccs | Q30 R65% |
Ihe nlere anya:
Nucleotides:
● osisi:
Mgbọrọgwụ, azuokokoosisi ma ọ bụ petal: 450 mg
Akwukwo ma obu nkpuru: 300 mg
Nkpuru: 1.2 g
Anụmanụ:
Obi ma ọ bụ eriri afọ: 300 mg
Vivalera ma ọ bụ ụbụrụ: 240 mg
Akwara: 450 mg
Ọkpụkpụ, ntutu ma ọ bụ akpụkpọ: 1G
● ●rropods:
Ụmụ ahụhụ: 6g
Crustacea: 300 mg
● Ọbara dum: 1 tube
Mkpụrụ ndụ: 106 seld
| CHAN. (NG / μL) | Ego (μg) | Nko | Eziokwu |
| 100 | ≥ 1.0 | OD260 / 280 = 1.7-25 OD260 / 230 = 0.5-2.5 Obere ma ọ bụ enweghị protein protein ma ọ bụ ihe mgbochi DNA gosipụtara na gel. | Maka osisi: rin≥7.5; Maka ụmụ anụmanụ: rin≥8.0; 5.0- 28s / 18s≥1.0; Obere ma ọ bụ enweghị ndabere |
Nnyefe nlele tụrụ aro
Akpati 2 ml centrifuge (tuhi anaghị atụ aro)
A na-edebe ihe nlele: Otu + Mepụta EG A1, A2, A3; B1, B2, B3.
Mbupu:
1
2. A ga-akpọtụ ụyọkọ: Enwere ike ịmịchasị ụrọ:
Ọrụ Ọrụ
Nchọpụta nnwale
Nyefe Inye Aka
Mmịpụta RNA
Ọbá Akwụkwọ Ewu
Sequencing
Nyocha data
Ọrụ Ahịa
Gụnyere nyocha ndị a:
Njikwa data dị mma
Nyochaa Nzuzo Polyadadenal (APA)
● ● Nnyocha transcript
● Nmapụta na-acha uhie uhie
● Enchchmaning Universal Universal Universal-Detuo Orthologs (Busco)
Nyochaa nyocha edegharị edepụta: amụma nke Sequeques (CD) na nyocha arụmọrụ
● LNCLNNA Nnyocha: Amụma nke LNCLNA na ebumnuche
● Nchọpụta Microselite (SSR)
Nyocha bọsco
Nnyocha ndị ọzọ
Ntụle mbụ nke polyAdenalylition (APA)
A na-arụ ọrụ na-arụ ọrụ nke transcript akwụkwọ
Nyochaa ọdụ ahụ na-eme ka ọrụ nke BMKGENENENENENENENTE zuru oke na mbipụta a gosipụtara.
Ma, y. et al. (2023) 'Nyocha nyocha nke Pacbio na OntT RNA usoro maka NEMOopiileMA VEMOPILA VECERILA VEMOPOM NIILE', Genomic, 115 (6), p. 110709. DI: 10,1016 / J.YGO.2023.110709.
Chao, Q. et al. (2019) 'Ógbè mmepe nke Ppulus Brem transcripticome', osisi Biochnology Jource, 17 (1), pp. 206-219. DOI: 10.1111 / PBI.12958.
DEng, H. et al. (2022) 'Dy ike acid acid na-adị n'oge mkpụrụ osisi actinidia (ihe ọkụkụ Accorbate-Mkpụrụ nke Actoral), 23 (10), p. 5808. DI: 10.3390 / ijms23105808 / S1.
Hua, X. et al. (2022) 'Ezi amụma nke mkpụrụ ndụ ihe nketa bishynletic nke biocylindlins na Paris Polyphylla', 1, 5 (1), pp. 1-10. DI: 10.1038 / S42003-022-03000-z.
Liu, m. et al. (2023) 'jikọtara Pacbio ISO-Sarch na Wardy Remalicomome na Tytock (Pytochrome P450 mkpụrụ ndụ', 14 (4), p. 363. DOI: 10.3390 / ụmụ ahụhụ14040363 / S1.
Wang, lijun et al. (2019) 'nyocha nke ntụgharị transcardome site na pacbio Synance - molecule na-achọpụta na a na-ahụ maka nghọta nke Ricnolec Activic acid', 20 (1), pp. 1-17. DI: 10.1186 / s12864-019-5832-9.
