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Usoro mgbochi mgbochi Chromatin (ChiP-seq)

Chromatin Immunoprecipitation (CHIP) bụ usoro na-enye ọgwụ mgbochi iji mee ka protein na-ejikọta DNA na ebumnuche ha kwekọrọ. Njikọ ya na NGS na-enyere aka ịkọwapụta genome-obosara nke ebumnuche DNA metụtara mgbanwe histone, ihe odide ederede, na protein ndị ọzọ na-ejikọta DNA. Ụzọ a siri ike na-enyere aka ntụnyere saịtị njikọ n'ofe ụdị cell, anụ ahụ, ma ọ bụ ọnọdụ dị iche iche. Ngwa ChIP-Seq sitere na ịmụ usoro ederede na ụzọ mmepe ruo n'ịkọwapụta usoro ọrịa, na-eme ka ọ bụrụ ngwa dị mkpa maka ịghọta mpaghara nhazi mkpụrụ ndụ ihe nketa yana ịkwalite nghọta ọgwụgwọ.

Platform: Illumina NovaSeq

Kpọtụrụ anyị

Nkọwa ọrụ

Bioinformatics

Nsonaazụ ngosi

Uru ọrụ

●Nyocha bioinformatic dị elu yana nkọwa zuru oke:Anyị na-eji ọtụtụ ọdụ data iji kọwaa mkpụrụ ndụ ihe nketa jikọtara ya na mpaghara njikọ protein-DNA, na-enye nghọta na usoro cellular na molecular na-akpata mmekọrịta.

●Nkwado mgbe ọrịre ahịa:Nkwenye anyị ga-agafe karịa mmecha ọrụ yana oge ọrụ ọnwa 3 gachara. N'ime oge a, anyị na-enye nlebanya ọrụ, enyemaka nchọpụta nsogbu, yana oge ajụjụ & A iji zaa ajụjụ ọ bụla metụtara nsonaazụ ya.

●Ahụmahụ sara mbara:Site na ndekọ egwu nke imecha ọtụtụ ọrụ ChIP-Seq nke ọma, ụlọ ọrụ anyị na-eweta ihe karịrị afọ iri nke nka na tebụl. Ndị otu nyocha anyị nwere nkà dị ukwuu, yana ọdịnaya zuru oke yana nkwado ọrịre ahịa, na-eme ka ọrụ gị nwee ọganihu.

● Njikwa ogo siri ike: Anyị na-emejuputa isi ihe nchịkwa n'ofe niile, site na nlele na nkwadebe ọbá akwụkwọ ruo usoro na bioinformatics. Nleba anya nke ọma a na-eme ka o doo anya na nnyefe nke nsonaazụ dị elu mgbe niile.