Nyocha serregrant
Uru bara uru
Takagit al., Akwụkwọ osisi ahụ, 2013
● Eziokwu: na-agwakọta 30 + 30 ruo narị mmadụ iri abụọ na narị mmadụ iji belata mkpọtụ dị iche iche; A na-eme ka ndị mmadụ na-enweghị usoro.
Nnyocha ● Nyochaa: Amalitere na-amamịghe onyinye onyinye na-arụ ọrụ, gụnyere NR, Switzersprot, gaa, Kgg, kog, wdg.
Oge ngwa ngwa ngwa ngwa: ngwa ngwa usoro dị ngwa n'ime ụbọchị 45.
Ahụmịhe ogologo: BMK enyela aka na ọtụtụ puku mmadụ, na-ekpuchi ụdị dị iche iche dịka ihe ọkụkụ, ngwaahịa aquatic, okooko osisi, wdg.
Nkọwapụta ọrụ
Onu ogugu:
Sergrating terogeny nke ndị nne na nna na-emegide phenotypes.
dịka f2 progeny, azụta (BC), na-akwụghachi ahịrị ahịrị (Ril)
Agwakọta ọdọ mmiri
N'ihi na njiri mara mmadụ: 30 ruo 50 mmadụ (opekempe 20) / Nnukwu
Maka nyocha nke Tratis: Top 5% ruo ndị mmadụ 10% nwere oke phenotypes na ọnụ ọgụgụ niile (opekempe 30 + 30).
Odide a na-atụ aro
Dịkarịa ala, nne ma ọ bụ nna na 1X / ụmụ maka ụmụ akwụkwọ na-agwakọta ọdọ mmiri 30 + 30
Bionamal nyocha
● Roofọdụ ihe odide
● Nhazi data
● Snp / indel na-akpọ
● Kọmputa Crowtite Echiche
● Nwanyị na-arụ ọrụ onyinye na-arụ ọrụ
Nlele Nlere Anya na Nnyefe
Ihe nlere anya:
Nucleotides:
| Sọle GDNA | Ihe Nleta Ahụ |
| Intata: ≥30 NG / μl | Osisi: 1-2 g |
| Ọnụ ego: ≥2 μg (voln ≥15 μl) | Anụmanụ: 0.5-1 g |
| Ọcha: OD260 / 280 = 1.6-2.5 | Ọbara dum: 1.5 ml |
Ọrụ Ọrụ
Nchọpụta nnwale
Nyefe Inye Aka
Mmịpụta RNA
Ọbá Akwụkwọ Ewu
Sequencing
Nyocha data
Ọrụ Ahịa
Ntọala nyocha 1. Icissaciations na anya dị anya (ED) iji mata mpaghara onye chọrọ. Na onu ogugu a
X-Axis: Nọmba chromosome; Nnyo ọ bụla na-anọchi anya uru nke snp bara uru. Black Line kwekọrọ na ọ bara uru. Uru dị elu dị elu na-egosi mkpakọrịta dị mkpa n'etiti saịtị ahụ na phenotpe. Line na-acha ọbara ọbara na-anọchite anya ọnụ ọgụgụ dị mkpa.
Nnyocha nyocha 2. IJI NA-EGO NA-EGO NA-EGO
X-Axis: Nọmba chromosome; DOT nke ọ bụla na-anọchi anya snan-index. Black Line guzoro maka nnabata SMP. Uru buru ibu bara uru bu, mkpakọrịta na.
Ikpe BMK
Nchọpụta ọnụọgụ abụọ dị ukwuu nke Lonl7.1 amanye mbubreyo embratenisis ọtụtụ protein jikọtara ya na kukumba
Nke bipụtara: Akuko biotechnology Jource, 2020
Usoro atụmatụ Sequency:
Ndị nne na nna (Jin5-508, yn): Usoro ihe odide niile nke mkpụrụ edemede maka 34 × na 20 ×.
Ọdọ mmiri DNA (Ogologo oge 50 dị mkpirikpi na 50)
Rụpụta nsonaazụ
N'ime ọmụmụ ihe a, segreging ọnụ ọgụgụ mmadụ (F2 na F2: 3) na-agafe ogologo ogo kukumba ahịrị ahịrị Jin5-50 na obere akara. Ọdọ mmiri DNA site na 50 dị ogologo na 50 oke mkpụmkpụ. Achọpụtara ihe dị mkpa QTL na Chrot7 site na nyocha BSA na nkewa ọdịnala QTL. Mpaghara onye na-anya onye na-anya ụgbọ elu dị warara site na ezigbo ihe, Gene okwu na-ekwupụta na nyocha ntụgharị, nke na-ekpughe igodo Gene na-achịkwa ogologo olu, csfnl7.1. Na mgbakwunye, polymorphism na CSFN7.1 A hụrụ mpaghara proroter na-akwado ya na ngosipụta kwekọrọ. Ọ ga-abụ na ọ ga-esi site na mpaghara FNL7.1 yikarịrị ka a ga-esi malite locus.
 QTL-Conpepp na nyocha BSA iji mata mpaghara nke chọrọ |  Lod profaịlụ nke kukumba olu-ogologo Qtl chọpụtara na Chrot7 |
XU, x., et al. "Ntọala dị ukwuu nke Lonl7.1 amanye na mbubreyo emytatesis ọtụtụ protein jikọtara ya na kukumba." Gosport osisi biochnology akwụkwọ 18.7 (2020).
