BMKMANU S1000 Spatial Transcriptome
Atụmatụ nka nka BMKMANU S1000 Spatial Transcriptome
Atụmatụ
● Mkpebi: 5 µM
● Ogologo ntụpọ: 2.5 µM
● Ọnụ ọgụgụ ntụpọ: ihe dịka nde abụọ
● Ụdị mpaghara 3 enwere ike ijide: 6.8 mm * 6.8 mm, 11 mm * 11 mm ma ọ bụ 15 mm * 20 mm
● A na-eju bead ọ bụla etinyere na primers nke nwere ngalaba anọ:
poly(dT) ọdụ maka mRNA priming na cDNA njikọ
Ihe nchọpụta molekụla pụrụ iche (UMI) iji dozie nhụsianya mmụba
Koodu oghere
Usoro njide nke akụkụ agụ 1 usoro primer
● H&E na fluorescent ntụpọ nke ngalaba
● Enwere ike ijiteknụzụ nkewa cell: mwekota nke H&E staining, fluorescent staining, na RNA sequencing iji chọpụta ókè nke ọ bụla cell na n'ụzọ ziri ezi ekenye mkpụrụ ndụ ihe nketa okwu na cell ọ bụla.
Uru nke BMKMANU S1000
●Mkpebi Sub-cellularMpaghara njide ọ bụla nwere> 2 nde oghere Barcoded Spots nwere dayameta nke 2.5 µm yana oghere nke 5 µm n'etiti ebe ntụpọ, na-enyere nyocha transcriptome ohere na mkpebi sub-cellular (5 µm).
●Nyocha mkpebi ọtụtụ ọkwa:Nyocha ọtụtụ ọkwa na-agbanwe agbanwe sitere na 100 μm ruo 5 μm iji dozie atụmatụ anụ ahụ dị iche iche na mkpebi kacha mma.
●Inwere ike iji teknụzụ nkewa mkpụrụ ndụ "Atọ n'ime otu slide":Ijikọta ntụpọ fluorescence, ntụpọ H&E, na usoro RNA n'otu slide, nyocha algorithm anyị "atọ n'ime otu" na-enye ike njirimara nke oke cell maka transcriptomics dabere na cell na-esote.
●Dakọtara na ọtụtụ usoro nhazi usoro: Ma NGS na usoro ọgụgụ ogologo dị.
●Nhazi Mgbanwe nke Mpaghara 1-8 Na-arụ Ọrụ: Ogo nke mpaghara ejidere na-agbanwe agbanwe, enwere ike iji usoro 3 (6.8 mm * 6.8 mm., 11 mm * 11 mm na 15 mm * 20 mm)
●Ọrụ nkwụsịtụ: Ọ na-ejikọta ahụmịhe niile na usoro dabere na nka, gụnyere ngalaba cryo-sectioning, staining, optimization tissue, spatial barcoding, nkwadebe ọbá akwụkwọ, usoro, na bioinformatics.
●Bioinformatics zuru oke yana nleba anya nke nsonaazụ ya:ngwugwu gụnyere nyocha 29 na ọnụ ọgụgụ dị elu 100+, jikọtara ya na iji ngwa ime ụlọ emepụtara iji hụ anya na hazie nkewa cell na nchịkọta ntụpọ.
●Nyochaa data ahaziri iche na nlegharị anya: dị maka arịrịọ nyocha dị iche iche
●Otu nka nka nke ukwuu: nwere ahụmahụ n'ihe karịrị ụdị anụ ahụ 250 na ụdị 100+ gụnyere mmadụ, òké, mammamal, azụ na osisi.
●Mmelite ozugbo na oru ngo niile: na njikwa zuru oke nke ọganihu nnwale.
●Nyocha njikọta nhọrọ yana usoro mRNA otu cell
Nkọwapụta ọrụ
|
Ihe atụ Ihe achọrọ
| Ụlọ akwụkwọ |
Usoro usoro
| akwadoro data | Njikwa ogo |
| Ihe nlele cryo nke agbakwunyere OCT, ihe mgbochi 3 kwa nlele | Ọbá akwụkwọ cDNA S1000 | Illumina PE150 (ọkwa ndị ọzọ dị) | 100K PE na-agụ kwa 100 uM (60-150 Gb) | RIN>7 |
Maka nkọwa ndị ọzọ na ntụzịaka nkwadebe nlele na usoro ọrụ ọrụ, biko nweere onwe gị ịgwa aỌkachamara BMKGENE
Usoro ọrụ ọrụ
N'oge nkwadebe nlele, a na-eme nnwale mmịpụta RNA mbụ iji hụ na enwere ike nweta RNA dị elu. N'ọkwa kachasị mma nke anụ ahụ, akụkụ ndị ahụ nwere ntụpọ ma na-ahụ anya ma na-emeziwanye ọnọdụ mRNA maka mwepụta anụ ahụ. A na-etinye usoro ahụ kachasị mma n'oge a na-arụ ụlọ ọba akwụkwọ, na-esote usoro na nyocha data.
Usoro ọrụ ọrụ zuru oke na-agụnye mmelite ozugbo yana nkwenye ndị ahịa iji nọgide na-enwe nzaghachi nzaghachi nzaghachi, na-eme ka mmezu ọrụ dị mma.
A na-enyocha data sitere na BMKMANU S1000 site na iji sọftụwia "BSTMatrix", nke BMKGENE mere onwe ya, na-emepụta Gene Expression Matrix. Site n'ebe ahụ, a na-emepụta akụkọ ọkọlọtọ nke gụnyere njikwa njirimara data, nyocha nke ime na nyocha nke otu.
● Njikwa ogo data:
Mmepụta data na nkesa akara ogo
Nchọpụta mkpụrụ ndụ ihe nketa kwa ntụpọ
Mkpuchi anụ ahụ
● Nleba anya n'ime:
Ọgaranya Gene
Ụyọkọ ntụpọ, gụnyere nleba anya nha nha
Nyocha okwu dị iche iche n'etiti ụyọkọ: njirimara nke mkpụrụ ndụ ihe nrịbama
Nkọwa ọrụ na ịba ụba nke mkpụrụ ndụ ihe nrịbama
● Nlebanya n'etiti otu:
Nchikota nke ntụpọ site na nlele abụọ (dịka ọrịa na njikwa) yana ụyọkọ ọzọ
Nchọpụta mkpụrụ ndụ ihe nrịbama maka ụyọkọ ọ bụla
Nkọwa ọrụ na ịba ụba nke mkpụrụ ndụ ihe nrịbama
Nkwuwapụta dị iche iche nke otu ụyọkọ n'etiti otu
Na mgbakwunye, BMKGENE mepụtara "BSTViewer" bụ ngwá ọrụ enyi na enyi nke na-enyere onye ọrụ aka iji anya nke uche hụ okwu mkpụrụ ndụ ihe nketa na ntụpọ nchịkọta na mkpebi dị iche iche.
BMKGene mepụtara sọftụwia maka nleba anya enyi na enyi
Nchịkọta ntụpọ BSTViewer na mkpebi ọtụtụ ọkwa
BSTCellViewer: Nkewa cell akpaka na akwụkwọ ntuziaka
Nleba anya n'ime
Nchịkọta ntụpọ:
Nchọpụta mkpụrụ ndụ ihe nrịbama na nkesa oghere:
Nyocha n'etiti otu
Ngwakọta data sitere na otu abụọ yana ụyọkọ ọzọ:
Mkpụrụ ndụ ihe nrịbama nke ụyọkọ ọhụrụ:
Jiri teknụzụ BMKManu S1000 nyochaa ọganihu nke ọrụ transcriptomics nke BMKGene na-akwado na mbipụta a egosipụtara:
Abụ, X. et al. (2023) 'Spatial transcriptomics na-ekpughe mkpụrụ ndụ chlorenchyma na-akpata ọkụ na-etinye aka n'ịkwalite mmụgharị ịse na callus tomato',Usoro nke National Academy of Sciences nke United States of America, 120 (38), p. e2310163120. doi: 10.1073/pnas.2310163120
Ị, Y. et al. (2023) 'Ntụle usoro nke usoro ederede gbasara ohere dabere na usoro',bioRxiv, p. 2023.12.03.569744. doi: 10.1101/2023.12.03.569744.
