16S / 18S / Emplicon sequencing-ngs
Atụmatụ Ọrụ
● Ọnọdụ Took: Reviaseq revaseq.
● Aclificationdị dị mkpirikpi mkpirisi 16s, 18s na ya, n'etiti ebumnuche nchekwa ndị ọzọ.
Nhọrọ Amplicon.
Ahụmịhe ọrụ ngo gara aga na ọtụtụ ebumnuche enwere ike.
Uru bara uru
●ISOLAtion-Free:Nchọpụta ngwa ngwa nke microbial mejupụtara na usoro gburugburu ebe obibi.
●Mkpebi dị elu: Na ngwa ala dị ala na gburugburu ebe obibi.
●Na-agbanwe agbanwe: Nchọpụta obodo microbial.
●Nnyocha Bionionformatic: Nchịkọta QII2 kachasị ọhụrụ na ngwa ọrụ dị iche iche na mgbatị nje microbial) nwere nyocha dị iche iche na usoro nchekwa data, akụkọ, OTV.
●Nnukwu nkasi ike: Na puku iri na ise Amplicon Serts na-eduzi kwa afọ, bmkgene na-eweta afọ iri, otu egwuregwu nyocha kachasị mma, na ezigbo nkwado na-akwado ahịa ahịa.
Nkọwapụta ọrụ
| Oba akwukwo | Atụmatụ sequencing | Data tụrụ aro |
| Ampicon | Ward Wardy Pe250 | 50/100 / 300k mkpado (Gụọ ụzọ) |
Ihe ị chọrọ
| Ileta (NG / μl) | Ọnụ ego (ng) | Olu (μl) |
| ≥1 | ≥200 | ≥20 |
● Ala / sludge: 1-2G
Anụmanụ na-ahụ maka ọdịnaya: 0,5-2g
● Ihe mejupụtara eriri afọ: 0.1-0.25g
● Osisi akuku (na-eme ka sediment): 0.1-0.5g
● fertation ofe ofe na-enye ndabara sediment): 0.1-0.5g
● Faces (nnukwu anụmanụ): 0,5-2g
Ikike (òké): 3-55grains
● Igwe ọtagelvage alvealary alvealary
● Swab Swab: 5-6 swabs
● Akpụkpọ anụ ahụ / ekenal / swab / swab / pharyngeal swab / reftal swab: 2-3 swab
● Igwe mkpuchi dị larịị: akwụkwọ nzacha
● Mee ihe / ikuku / biofilm: akwụkwọ nzacha
● Monphytes: 1-2G
● Ihe ncheta ezé: 0.5-1g
Ọrụ Ọrụ
Nyefe Inye Aka
Ọbá Akwụkwọ Ewu
Sequencing
Nyocha data
Ọrụ Ahịa
Gụnyere nyocha ndị a:
- Njikwa data dị mma
- OTU columting / De-Noick (ASV)
- OTU akụkọ
- Nyochaa NPPA iche iche: ọtụtụ ndebiri, gụnyere Shannon, Simpson na Ace.
- Nyocha ụdị nyocha Beta
- Nnyocha nke otu
- Nnyocha mmezi: n'etiti ihe ndị metụtara gburugburu ebe obibi na ihe ndị dị iche iche
- Atụmatụ ọgụgụ isi iri na isii
Ntachi anya nke nkesa na-arụ ọrụ
Nlele anya nke ukwuu
Nyochaa Ntanetị Alfa: Usoro Nzuzo
Nyocha nyocha nke Beta: nmds
Nchọpụta Intertation: Left Leaomarkorder
Nyochaa ọdụ ahụ na-eme ka ọrụ Amplgene nke BMKEN na-arụ ọrụ na Wardy site na nchịkọta nke akwụkwọ achọrọ.
Dong, C. et al. (2022) 'Achịkọta Microbuta, na Ọrụ nke Rhizonatio na Microjiia Ulmoiology'. DI: 10.3389 / FMICB.2022.855317 / zuru.
Li, y. et al. (2023) 'Sontikmụrụ nje na-emegharị maka ọgwụgwọ nke Gardneel Vatectionalsis na-enye ọgwụ ọjọọ na ụmụ oke', microbime, 11-14. DI: 10.1186 / S40168-023-01497-Y
Yang, J., FU, Y. na Liu, H. (2022) 1-2. DI: 10.1186 / S40793-022-00399-0 / onu ogugu 8.
Yin, s. et al. (2022) 'fortsStock-Ndọrọ ókè ala nke mkpụrụ ndụ ihe ndị metụtara nitrogen na-achịkwa nitrogen na-achịkwa nitrogen na-achịkwa nitrogen na composen', teknụzụ Buorsource, 361, p61, p. 127678. DI: 10,1016 / JI.Biortech.2022.127678.
