Asanble genomic T2T, Gap gratis genomic
1stDe jnom diri1
Tit: Asanble ak Validasyon nan de Gap-gratis referans jnom pou Xian/Indica Rice revele Sur nan plant achitekti Centromere
Doi:https://doi.org/10.1101/2020.12.24.424073
Moun ki afiche li a: 01 janvye 2021.
Enstiti: Huazhong Inivèsite Agrikòl, Lachin
Materyèl
O. sativa xian/indicaZhenshan 97 (Zs97) Varyete Rice 'ak' Minghui 63 (MH63)
Estrateji sekans
NGS li + HIFI li + CLR li + Bionano + Hi-C
Done:
ZS97: 8.34 GB （~ 23x） HIFI li + 48,39 GB (~ 131X) CLR li + 25 GB (~ 69X) NGS + 2 Bionano IRYS selil
MH63: 37.88 GB (~ 103X) HIFI li + 48,97 GB (~ 132x) CLR li + 28 GB (~ 76x) NGS + 2 Bionano IRYS selil
Figi 1 De Gap-gratis jnom nan diri (MH63 ak ZS97)
2ndGenomic bannann2
Tit: Telomere-a-telomè gapless kwomozòm nan bannann lè l sèvi avèk nanopore sekans
Doi:https://doi.org/10.1101/2021.04.16.440017
Moun ki afiche li a: 17 avril, 2021.
Enstiti: Université Paris-Saclay, Lafrans
Materyèl
Double haploidMusa acuminatasppmalaccensis(Dh-pahang)
Estrateji sekans ak done:
HISEQ2500 PE250 MODE+ MINION/ PROMETHION (93GB, ~ 200X)+ kat optik (DLE-1+ BSPQ1)
Tablo 1 Konparezon Musa Acuminata (DH-Pahang) Asanble genomic
Figi 2 Musa Jenom Achitèk Konparezon
3rdPhaeodactylum tricornutum genomic3
Tit: Telomere-a-telomè asanble genomic nanP
haeodactylum tricornutum
Doi:https://doi.org/10.1101/2021.05.04.442596
Afiche tan: Me 04, 2021
Enstiti: Western University, Kanada
Materyèl
Phaeodactylum tricornutum(Koleksyon Kilti nan alg ak Protozoa CCAP 1055/1)
Estrateji sekans ak done:
1 Oxford Nanopore Minion Flow Cell + A 2 × 75 pè-fen mitan-pwodiksyon NextSeq 550 kouri
Figi 3 workflow pou telomere-a-telomè asanble genomic
4thImèn CHM13 genomic4
Tit: sekans konplè yon genomic imen
Doi:https://doi.org/10.1101/2021.05.26.445798
Moun ki afiche li a: 27 Me, 2021
Enstiti: Enstiti Nasyonal Sante (NIH), USA
Materyèl: liy selil CHM13
Estrateji sekans ak done:
30 × PacBio sekans konsansis sikilè (HIFI), 120 × Oxford Nanopore ultra-long li sekans, 100 × Illumina PCR-gratis sekans (ILMN), 70 × Illumina / Arima Genomics Hi-C (Hi-C), Bionano kat optik, ak Strand-seq
Tablo 2 Konparezon GRCH38 ak T2T-CHM13 Asanble genomic imen
Mansyon
1.Sergey Nurk et al. Sekans konplè yon genomic imen. Biorxiv 2021.05.26.445798; doi:https://doi.org/10.1101/2021.05.26.445798
2.Caroline Belser et al. Telomere-a-telomè kwomozòm gapless nan bannann lè l sèvi avèk sekans nanopore. Biorxiv 2021.04.16.440017; doi:https://doi.org/10.1101/2021.04.16.440017
3.Daniel J. Giguere et al. Telomere-a-telomè asanble genomè nan Phaeodactylum tricornutum. Biorxiv 2021.05.04.442596; doi:https://doi.org/10.1101/2021.05.04.442596
4.jia-Ming Song et al. Asanble ak validasyon nan de gap referans diferans-gratis pou Xian/Indica Rice revele Sur nan achitekti plant Centromere. Biorxiv 2020.12.24.424073; doi:https://doi.org/10.1101/2020.12.24.424073
Post tan: Jan-06-2022