Kev Tiv Thaiv Me Me RNA
Nta
● Kev npaj lub tsev qiv ntawv suav nrog ib qho kev xaiv me me
● Bioinformatic tsom xam nyob rau ib puag ncig Merma twv ua ntej thiab lawv lub hom phiaj
Kev Pabcuam Zoo
●Kev txheeb xyuas qhov tseeb:Kev ua kom paub cov cim ntawm ob qho tib si paub thiab paub cov hom phiaj mirnas, thiab coj cov ntawv sau ua haujlwm thiab txhawb nrog ntau yam databases (kegg, mus)
●Kev tswj kom nruj: Peb siv cov ntsiab lus tseem ceeb thoob plaws txhua theem, los ntawm cov qauv thiab chaw khaws ntawv npaj rau kev sib txuas thiab bioinformics. Qhov kev saib xyuas me me no ua kom pom tseeb qhov kev xa tawm ntawm cov txiaj ntsig zoo.
●Kev them nyiaj yug tom qab muag: Peb cov kev cog lus txuas ntxiv tshaj qhov ua tiav nrog 3-hli tom qab muag lub sijhawm pabcuam. During this time, we offer project follow-up, troubleshooting assistance, and Q&A sessions to address any queries related to the results.
●Kev txawj ntse dav: Nrog rau cov ntaub ntawv teev tseg ntawm kev kaw tau zoo tshaj li ntau tus SRNS cov kev tshawb fawb tshaj 300 hom nyob rau ntau pab neeg coj kev paub rau txhua qhov project.
Qauv yuav tsum tau ua thiab xa khoom
| Lub tsev qiv ntawv | Sam thiaj | Cov ntaub ntawv pom zoo | Cov ntaub ntawv QC |
| Loj raug xaiv | Thaum pom ICAINA | 10M-20m nyeem | Q30-85% |
Qauv Yuav Tsum Tau Ua:
Nucleotides:
| Conc. (NG / μl) | Tus nqi (μg) | Kev coj dawb huv | Kev muaj siab dawb paug |
| ≥ 80 | ≥ 0.8 | OD260 / 280 = 1.7-2.5 OD260 / 230 = 0.5-25-2.5 Txwv tsis pub lossis tsis muaj protein los yog cov khoom tsis huv uas muaj nyob ntawm gel. | Rin≥6.0; 5.0 ≥28s / 18S≥1.0; Txwv tsis pub lossis tsis muaj qhov nce hauv qab |
● ● Nroj tsuag:
Hauv paus, kav lossis Petal: 450 mg
Nplooj lossis noob: 300 mg
Txiv Hmab Txiv Ntoo: 1.2 g
Tsiaj:
Lub plawv lossis txoj hnyuv: 450 mg
Viscera lossis Hlwb: 240 mg
Cov leeg: 600 mg
Cov pob txha, plaub hau lossis tawv nqaij: 1.5g
Arthropods:
Kab: 9G
Crustacea: 450 mg
Lub ntshav tag nrho: 2 hlab
● Cells: 106 ig
● Ntshav dej thiab ntshav:6 ml
Pom zoo tus qauv xa mus
Thawv: 2 Ml Centrifuge Tube (Tin Ntawv Nyiaj tsis pom zoo)
Qauv kos npe: Pab pawg + hloov kho dua li AG1, A2, A3; B1, B2, B3.
Cov zaub mov xa tuaj:
1. Cov dej khov txias: Cov qauv yuav tsum tau ntim rau hauv cov hnab thiab faus rau hauv cov dej khov qhuav.
2. Rnastable Hlis: RNA cov qauv tuaj yeem qhuav hauv RNA stabilization raj (piv txwv li rnastable®) thiab xa tawm hauv chav tsev kub.
Kev Pabcuam Khiav
Kev Tshawb Fawb Kev Tshawb Fawb
Qauv Xa
RNA rho tawm
Kev Siv Hauv Tsev Qiv Ntawv
Ze
Cov Ntaub Ntawv Kev Ntsuam Xyuas
Cov Kev Pab Tom Qab Muag
Bioinformformic
● Cov ntaub ntawv nyoos Zoo tswj
● Srna Kev Ua Haujlwm
● Raws li tus siv genome
● Qhia txog paub thiab tshiab Mirna
● Kev txheeb xyuas Mirna Sib Txawv
● Cov kev ua haujlwm tshwj xeeb ntawm Mirna Hom Phiaj
Kev cim ntawm Mirna: Cov qauv thiab qhov tob
Kev nthuav tawm ntau ntawm Mirna - Honashical clustering
Kev ua haujlwm tshwj xeeb ntawm cov phiaj ntawm kev sib txawv ntawm cov txuj ci tseem ceeb
Tshawb cov kev tshawb fawb txog kev tshawb fawb los ntawm BMKGene 'SRNA cov kev pab cuam sib txuas los ntawm kev sau cov ntaub ntawv zoo nkauj.
Chen, H. li al. (2023) 'Tus kab mob kis tau Inhibit Saponin Biosynthesis thiab Phothanthesis hauv Panax Nabinseng', Kev Cog Hluav Taws Xob thiab Biochemistry, 203, p. 108038. DOI: 10.1016 / J.PRAPY.2023.1.1.
Li, H. li al. (2023) 'Tus nroj tsuag FYVE Domain-muaj protein dawb1 koom nrog microprocessor cov khoom siv rau kev ua tsis pub tshaj tawm Mirna Biogenesis', Embo Ntawv Tshaj Qhia, 24). DOI: 10.15252 / paj tau 5.202255037 / txuas_File / POST_7-sup-0004-sdatafig4.tif.
YU, J. li al. (2023) 'Lub microrna Ame-Bantam-3p Tswj Laral Vis Kev Loj Hlob los ntawm kev sib tw ntawm cov muv kev loj hlob-zoo li tus lej honey) Cov. 5726. DOI: 10.3390 / iJMS24065726 / s1.
Zhang, M. li al. (2018) 'Cov noob ntsuas sib xyaw ntawm Mirna thiab cov nqaij muaj feem cuam tshuam rau cov qaib', lub cev muaj ntsha thiab biochemistry, 46 (6), pp. 2421-2433. DOI: 10.1159 / 000489649.
