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BMKMANU S3000_Spatial Transcriptome
Spatial transcriptomics sawv ntawm qhov ua ntej ntawm kev tshawb fawb txog kev tsim kho tshiab, txhawb cov kws tshawb fawb kom delve rau hauv cov qauv ntawm cov noob caj noob ces hauv cov ntaub so ntswg thaum khaws cia lawv cov ntsiab lus spatial. Nyob nruab nrab ntawm ntau lub platform, BMKGene tau tsim BMKManu S3000 Spatial Transcriptome Chip, khav theeb qhov kev daws teeb meem zoo dua ntawm 3.5µm, mus txog qhov subcellular ntau yam, thiab ua kom muaj ntau theem kev daws teeb meem. Lub S3000 nti, uas muaj kwv yees li 4 lab qhov chaw, ntiav microwells txheej nrog cov hlaws dai nrog spatially barcoded capture probes. Lub tsev qiv ntawv cDNA, txhawb nqa nrog spatial barcodes, tau npaj los ntawm S3000 nti thiab tom qab ntawd tau ua tiav ntawm Illumina NovaSeq platform. Kev sib xyaw ua ke ntawm spatial barcoded qauv thiab UMIs ua kom qhov tseeb thiab tshwj xeeb ntawm cov ntaub ntawv tsim tawm. BMKManu S3000 nti yog qhov muaj ntau yam, muab ntau theem kev daws teeb meem uas tuaj yeem hloov kho kom zoo rau cov ntaub so ntswg sib txawv thiab cov qib xav tau nthuav dav. Qhov kev hloov pauv no tso lub nti ua qhov kev xaiv zoo tshaj plaws rau ntau yam kev tshawb fawb spatial transcriptomics, kom ntseeg tau meej qhov sib cais nrog cov suab nrov tsawg. Kev siv ntawm tes segmentation thev naus laus zis nrog BMKManu S3000 ua rau qhov kev txwv ntawm cov ntaub ntawv hloov pauv mus rau thaj tsam ntawm cov hlwb, ua rau muaj kev tshuaj xyuas uas muaj lub ntsiab lus ncaj qha lom neeg. Tsis tas li ntawd, kev txhim kho kev daws teeb meem ntawm S3000 ua rau muaj cov noob ntau dua thiab UMIs kuaj pom hauv ib lub xovtooj ntawm tes, ua rau muaj kev tshuaj xyuas ntau dua ntawm cov qauv kev hloov pauv ntawm qhov chaw thiab pawg cell.
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Tib- nuclei RNA Sequencing
Txoj kev loj hlob ntawm ib leeg-cell capture thiab kev cai tsev qiv ntawv tsim cov tswv yim, ua ke nrog high-throughput sequencing, tau revolutionized gene qhia kev kawm nyob rau hauv lub cell. Qhov kev rhuav tshem no tso cai rau kev sib sib zog nqus thiab kev tshuaj xyuas ntau dua ntawm cov cell complex, kov yeej cov kev txwv cuam tshuam nrog qhov nruab nrab ntawm cov noob qhia txog txhua lub hlwb thiab khaws cia qhov tseeb heterogeneity nyob rau hauv cov pejxeem. Thaum ib leeg-cell RNA sequencing (scRNA-seq) muaj qhov zoo tsis txaus ntseeg, nws ntsib cov teeb meem hauv qee cov ntaub so ntswg uas tsim cov kev ncua ntawm ib leeg ua pov thawj nyuaj thiab xav tau cov qauv tshiab. Ntawm BMKGene, peb hais txog qhov teeb meem no los ntawm kev muab ib lub nucleus RNA sequencing (snRNA-seq) siv lub xeev-of-the-art 10X Genomics Chromium thev naus laus zis. Txoj hauv kev no nthuav dav cov spectrum ntawm cov qauv ua kom zoo rau kev txheeb xyuas cov ntaub ntawv ntawm qib ib leeg.
Kev sib cais ntawm nuclei yog ua tiav los ntawm lub tshiab 10X Genomics Chromium nti, uas muaj yim-channel microfluidics system nrog ob txoj kev hla. Nyob rau hauv cov txheej txheem no, gel hlaws dai nrog barcodes, primers, enzymes, thiab ib lub nucleus yog encapsulated nyob rau hauv nanoliter-loj roj tee, tsim Gel Bead-in-Emulsion (GEM). Tom qab GEM tsim, cell lysis thiab barcode tso tawm tshwm sim hauv txhua GEM. Tom qab ntawd, mRNA molecules tau thim rov qab mus rau hauv cDNAs, suav nrog 10X barcodes thiab Cim Molecular Identifiers (UMIs). Cov cDNAs no tom qab ntawd raug rau cov qauv kev tsim kho tsev qiv ntawv, ua kom yooj yim rau kev tshawb nrhiav cov noob caj noob ces ntawm qib ib leeg.
Platform: 10 × Genomics Chromium thiab Illumina NovaSeq Platform
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10x Genomics Visium Spatial Transcriptome
Spatial transcriptomics yog ib qho kev siv thev naus laus zis uas tso cai rau cov kws tshawb fawb los tshawb xyuas cov qauv ntawm cov noob hauv cov ntaub so ntswg thaum khaws cia lawv cov ntsiab lus spatial. Ib lub platform muaj zog hauv qhov no yog 10x Genomics Visium ua ke nrog Illumina sequencing. Lub hauv paus ntsiab lus ntawm 10X Visium yog nyob ntawm ib qho tshwj xeeb nti nrog rau qhov chaw ntes uas tau muab tso rau hauv cov ntaub so ntswg. Qhov chaw ntes no muaj cov chaw barcoded, txhua qhov sib xws rau qhov chaw tshwj xeeb hauv cov ntaub so ntswg. Lub ntes RNA molecules los ntawm cov ntaub so ntswg yog tom qab ntawd sau nrog cov cim molecular identifiers (UMIs) thaum lub sijhawm rov qab cov txheej txheem. Cov chaw barcoded thiab UMIs pab kom meej spatial mapping thiab kom muaj nuj nqis ntawm cov noob qhia ntawm ib leeg-cell daws teeb meem. Kev sib xyaw ua ke ntawm spatial barcoded qauv thiab UMIs ua kom qhov tseeb thiab tshwj xeeb ntawm cov ntaub ntawv tsim tawm. Los ntawm kev siv thev naus laus zis Spatial Transcriptomics, cov kws tshawb fawb tuaj yeem nkag siab tob txog lub koom haum spatial ntawm cov hlwb thiab cov kev sib cuam tshuam ntawm cov molecular uas tshwm sim hauv cov ntaub so ntswg, muab kev nkag siab zoo rau cov txheej txheem hauv cov txheej txheem lom neeg hauv ntau qhov chaw, suav nrog oncology, neuroscience, kev loj hlob biology, immunology. , thiab kev tshawb fawb botanical.
Platform: 10X Genomics Visium thiab Illumina NovaSeq
