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DNBSEQ cov tsev qiv ntawv ua ntej
DNBSEQ, tsim los ntawm MGI, yog ib qho tshiab NGS thev naus laus zis uas tau tswj kom txo qis cov nqi sib txuas ntxiv thiab nce kev nce qib. Kev npaj ntawm DNBSEQ cov tsev qiv ntawv suav nrog DNA fragmentation, kev npaj ntawm ssDNA, thiab dov lub voj voog kom tau txais DNA nanoballs (DNB). Cov no yog tom qab ntawd thauj mus rau qhov chaw ruaj khov thiab tom qab sib txuas los ntawm combinatorial Probe-Anchor Synthesis (cPAS). DNBSEQ thev naus laus zis sib xyaw ua ke qhov zoo ntawm qhov muaj qhov tsis zoo ntawm kev ua yuam kev tsawg nrog kev siv cov qauv kev ua yuam kev siab nrog nanoballs, uas ua rau muaj kev sib txuas nrog ntau dua thiab qhov tseeb.
Peb lub tsev qiv ntawv ua ntej ua ke ua ke ua rau cov neeg siv khoom npaj Illumina sequencing cov tsev qiv ntawv los ntawm ntau qhov chaw (mRNA, tag nrho cov genome, amplicon, 10x cov tsev qiv ntawv, thiab lwm yam), uas tau hloov mus rau MGI cov tsev qiv ntawv hauv peb lub chaw soj nstuam kom ua tiav hauv DNBSEQ-T7, ua rau cov ntaub ntawv siab ntawm cov nqi qis.
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Lub tsev qiv ntawv Illumina ua ntej
Illumina sequencing thev naus laus zis, raws li Sequencing los ntawm Synthesis (SBS), yog lub ntiaj teb puag ncig NGS kev tsim kho tshiab, lub luag haujlwm tsim tawm ntau dua 90% ntawm lub ntiaj teb cov ntaub ntawv sequencing. Lub hauv paus ntsiab lus ntawm SBS suav nrog cov duab fluorescently sau npe rov qab cov terminators raws li txhua dNTP tau ntxiv, thiab tom qab ntawd tau txiav kom tso cai rau kev koom ua ke ntawm lub hauv paus tom ntej. Nrog rau tag nrho plaub qhov thim rov qab-txhim kho dNTPs tam sim no nyob rau hauv txhua lub voj voog sib luag, kev sib tw ntuj txo qis kev sib koom ua ke. Qhov kev siv thev naus laus zis no txhawb nqa ob qho tib si nyeem ib leeg thiab ua ke-kawg cov tsev qiv ntawv, ua rau ntau yam kev siv genomic. Illumina sequencing's high-throughput peev xwm thiab precision qhov chaw nws yog lub hauv paus hauv kev tshawb fawb genomics, txhawb cov kws tshawb fawb los daws qhov tsis sib xws ntawm cov genomes nrog cov ntsiab lus tsis sib xws thiab kev ua haujlwm zoo.
Peb lub tsev qiv ntawv ua ntej ua ke ua ke ua rau cov neeg siv khoom npaj cov tsev qiv ntawv sib txuas los ntawm ntau qhov chaw (mRNA, tag nrho cov genome, amplicon, 10x cov tsev qiv ntawv, thiab lwm yam). Tom qab ntawd, cov tsev qiv ntawv no tuaj yeem raug xa mus rau peb cov chaw sib txuas ua ke rau kev tswj xyuas zoo thiab kev sib txuas hauv Illumina platforms.
