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Sib piv Genomics
Kev sib piv genomics suav nrog kev tshuaj xyuas thiab kev sib piv ntawm tag nrho cov genome sequences thiab cov qauv ntawm ntau hom. Daim teb no nrhiav kev nthuav tawm cov evolution ntawm hom, txiav txim siab gene functions, thiab elucidate cov genetic regulatory mechanisms los ntawm kev txheeb xyuas cov conserved los yog divergent sequence qauv thiab cov ntsiab lus nyob rau hauv ntau yam kab mob. Kev tshawb fawb txog kev sib piv genomics suav nrog kev txheeb xyuas xws li cov tsev neeg cov noob, kev txhim kho kev hloov pauv, tag nrho-genome duplication txheej xwm, thiab qhov cuam tshuam ntawm cov kev xaiv xaiv.
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Evolutionary noob caj noob ces
Cov pej xeem thiab evolutionary genetic tsom xam platform yog tsim los ntawm lub hauv paus ntawm loj heev kev sau nyob rau hauv BMK R & D pab neeg rau xyoo. Nws yog cov cuab yeej siv tus phooj ywg tshwj xeeb tshaj yog rau cov kws tshawb fawb uas tsis loj hauv bioinformatics. Lub platform no tso cai rau cov kev hloov pauv ntawm cov noob caj noob ces cuam tshuam txog kev txheeb xyuas qhov tseem ceeb suav nrog kev tsim kho tsob ntoo phylogenetic, kev sib txuas tsis sib xws, kev ntsuam xyuas kev sib txawv ntawm caj ces, xaiv cheb tsom xam, txheeb xyuas kev sib txheeb, PCA, kev txheeb xyuas pej xeem cov qauv, thiab lwm yam.
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Hi-C Based Genome Assembly
Hi-C yog ib txoj hauv kev tsim los ntes chromosome configuration los ntawm kev sib txuas kev soj ntsuam raws li kev sib cuam tshuam thiab kev sib tw siab. Qhov kev siv ntawm cov kev sib cuam tshuam no ntseeg tau tias muaj kev cuam tshuam tsis zoo nrog lub cev nyob deb ntawm chromosomes. Yog li ntawd, Hi-C cov ntaub ntawv yog siv los coj cov pawg, kev txiav txim, thiab kev taw qhia ntawm cov kab ke sib dhos hauv cov txheej txheem genome thiab khi rau cov uas mus rau qee tus lej ntawm chromosomes. Cov thev naus laus zis no txhawb nqa lub chromosome-theem genome los ua ke thaum tsis muaj daim ntawv qhia txog noob caj noob ces. Txhua lub genome xav tau Hi-C.
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Nroj / Tsiaj De Novo Genome Sequencing
De Novosequencing yog hais txog kev tsim kho ntawm ib hom genome tag nrho siv cov thev naus laus zis sib txuas hauv qhov tsis muaj kev siv genome. Kev taw qhia thiab kev siv dav dav ntawm kev sib txuas thib peb, uas muaj kev nyeem ntawv ntev dua, tau txhim kho genome los ua ke los ntawm kev nce qhov sib tshooj ntawm kev nyeem. Qhov kev txhim kho no tshwj xeeb tshaj yog cuam tshuam thaum cuam tshuam nrog cov genomes nyuaj, xws li cov uas muaj heterozygosity siab, qhov sib piv siab ntawm cov cheeb tsam rov qab, polyploids, thiab cov cheeb tsam nrog cov khoom rov ua dua, cov ntsiab lus tsis zoo ntawm GC, lossis cov complexity siab uas feem ntau tsis zoo sib sau ua ke siv luv luv nyeem sequencing. ib leeg.
Peb qhov kev daws teeb meem ib leeg muab cov kev pabcuam sib txuas ua ke thiab kev tshuaj xyuas bioinformatic uas xa cov khoom zoo ntawm novo sib sau ua ke genome. Thawj qhov kev tshawb fawb genome nrog Illumina muab kev kwv yees ntawm genome loj thiab nyuaj, thiab cov ntaub ntawv no yog siv los coj cov kauj ruam tom ntej ntawm kev nyeem ntawv ntev nrog PacBio HiFi, ua raws li.de novsib dhos ntawm contigs. Kev siv tom ntej ntawm HiC sib dhos ua kom muaj kev sib txuas ntawm cov kab mob mus rau genome, tau txais chromosome-theem sib dhos. Thaum kawg, lub genome yog piav qhia los ntawm kev kwv yees cov noob thiab los ntawm kev sib txuas cov noob caj noob ces, hloov mus rau cov ntawv sau nrog luv luv thiab ntev nyeem.
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Human Whole Exome Sequencing
Human Whole exome sequencing (hWES) yog dav lees paub tias yog ib qho txiaj ntsig zoo thiab muaj zog sib txuas rau kev txheeb xyuas cov kab mob uas ua rau muaj kev hloov pauv. Txawm hais tias tsuas yog kwv yees li 1.7% ntawm tag nrho cov genome, exons ua lub luag haujlwm tseem ceeb los ntawm kev cuam tshuam ncaj qha rau qhov profile ntawm tag nrho cov protein ua haujlwm. Qhov tseem ceeb, hauv tib neeg genome, ntau dua 85% ntawm kev hloov pauv cuam tshuam nrog cov kab mob tshwm sim hauv thaj tsam protein coding. BMKGENE muab cov kev pabcuam dav dav thiab hloov tau yooj yim rau tib neeg tag nrho exome sequencing kev pabcuam nrog ob qhov sib txawv exon ntes cov tswv yim muaj los ua kom tau raws li ntau yam kev tshawb fawb lub hom phiaj.
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Specific-Locus Amplified Fragment Sequencing (SLAF-Seq)
High-throughput genotyping, tshwj xeeb tshaj yog nyob rau hauv loj-scale pej xeem, yog ib qho tseem ceeb kauj ruam nyob rau hauv kev tshawb fawb genetic koom haum thiab muab ib tug genetic hauv paus rau kev ua hauj lwm gene discovery, evolutionary tsom xam, thiab lwm yam. Tsis txhob sib sib zog nqus tag nrho genome re-sequencing,Txo Cov Neeg Sawv Cev Genome Sequencing (RRGS)feem ntau yog ua hauj lwm hauv cov kev tshawb fawb no kom txo qis tus nqi sequencing ib tus qauv thaum tswj kev ua haujlwm tsim nyog hauv kev tshawb nrhiav caj ces. RRGS ua tiav qhov no los ntawm kev zom cov DNA nrog kev txwv cov enzymes thiab tsom mus rau qhov tshwj xeeb fragment loj ntau yam, yog li sequencing tsuas yog ib feem ntawm cov genome. Ntawm cov txheej txheem RRGS ntau yam, Specific-Locus Amplified Fragment Sequencing (SLAF) yog ib txoj hauv kev kho kom haum thiab zoo. Txoj kev no, tsim nws tus kheej los ntawm BMKGene, optimizes txwv enzyme teem rau txhua qhov project. Qhov no ua kom muaj ntau tus lej ntawm SLAF cov cim npe (400-500 bps cheeb tsam ntawm cov genome tau ua ntu zus) uas tau muab faib ua ke thoob plaws lub genome thaum ua haujlwm kom tsis txhob rov ua dua thaj chaw, yog li ua pov thawj qhov zoo tshaj plaws genetic marker discovery.
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Lub tsev qiv ntawv Illumina ua ntej
Illumina sequencing thev naus laus zis, raws li Sequencing los ntawm Synthesis (SBS), yog lub ntiaj teb puag ncig NGS kev tsim kho tshiab, lub luag haujlwm tsim tawm ntau dua 90% ntawm lub ntiaj teb cov ntaub ntawv sequencing. Lub hauv paus ntsiab lus ntawm SBS suav nrog cov duab fluorescently sau npe rov qab cov terminators raws li txhua dNTP tau ntxiv, thiab tom qab ntawd tau txiav kom tso cai rau kev koom ua ke ntawm lub hauv paus tom ntej. Nrog rau tag nrho plaub qhov thim rov qab-txhim kho dNTPs tam sim no nyob rau hauv txhua lub voj voog sib luag, kev sib tw ntuj txo qis kev sib koom ua ke. Qhov kev siv thev naus laus zis no txhawb nqa ob qho tib si nyeem ib leeg thiab ua ke-kawg cov tsev qiv ntawv, ua rau ntau yam kev siv genomic. Illumina sequencing's high-throughput peev xwm thiab precision qhov chaw nws yog lub hauv paus hauv kev tshawb fawb genomics, txhawb cov kws tshawb fawb los daws qhov tsis sib xws ntawm cov genomes nrog cov ntsiab lus tsis sib xws thiab kev ua haujlwm zoo.
Peb lub tsev qiv ntawv ua ntej ua ke ua ke ua rau cov neeg siv khoom npaj cov tsev qiv ntawv sib txuas los ntawm ntau qhov chaw (mRNA, tag nrho cov genome, amplicon, 10x cov tsev qiv ntawv, thiab lwm yam). Tom qab ntawd, cov tsev qiv ntawv no tuaj yeem raug xa mus rau peb cov chaw sib txuas ua ke rau kev tswj xyuas zoo thiab kev sib txuas hauv Illumina platforms.
