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BMKMANU S3000_Spatial Transcriptome
Spatial transcriptomics sawv ntawm qhov ua ntej ntawm kev tshawb fawb txog kev tsim kho tshiab, txhawb cov kws tshawb fawb kom delve rau hauv cov qauv ntawm cov noob caj noob ces hauv cov ntaub so ntswg thaum khaws cia lawv cov ntsiab lus spatial. Nyob nruab nrab ntawm ntau lub platform, BMKGene tau tsim BMKManu S3000 Spatial Transcriptome Chip, khav theeb qhov kev daws teeb meem zoo dua ntawm 3.5µm, mus txog qhov subcellular ntau yam, thiab ua kom muaj ntau theem kev daws teeb meem. Lub S3000 nti, uas muaj kwv yees li 4 lab qhov chaw, ntiav microwells txheej nrog cov hlaws dai nrog spatially barcoded capture probes. Lub tsev qiv ntawv cDNA, txhawb nqa nrog spatial barcodes, tau npaj los ntawm S3000 nti thiab tom qab ntawd tau ua tiav ntawm Illumina NovaSeq platform. Kev sib xyaw ua ke ntawm spatial barcoded qauv thiab UMIs ua kom qhov tseeb thiab tshwj xeeb ntawm cov ntaub ntawv tsim tawm. BMKManu S3000 nti yog qhov muaj ntau yam, muab ntau theem kev daws teeb meem uas tuaj yeem hloov kho kom zoo rau cov ntaub so ntswg sib txawv thiab cov qib xav tau nthuav dav. Qhov kev hloov pauv no tso lub nti ua qhov kev xaiv zoo tshaj plaws rau ntau yam kev tshawb fawb spatial transcriptomics, kom ntseeg tau meej qhov sib cais nrog cov suab nrov tsawg. Kev siv ntawm tes segmentation thev naus laus zis nrog BMKManu S3000 ua rau qhov kev txwv ntawm cov ntaub ntawv hloov pauv mus rau thaj tsam ntawm cov hlwb, ua rau muaj kev tshuaj xyuas uas muaj lub ntsiab lus ncaj qha lom neeg. Tsis tas li ntawd, kev txhim kho kev daws teeb meem ntawm S3000 ua rau muaj cov noob ntau dua thiab UMIs kuaj pom hauv ib lub xovtooj ntawm tes, ua rau muaj kev tshuaj xyuas ntau dua ntawm cov qauv kev hloov pauv ntawm qhov chaw thiab pawg cell.
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DNBSEQ cov tsev qiv ntawv ua ntej
DNBSEQ, tsim los ntawm MGI, yog ib qho tshiab NGS thev naus laus zis uas tau tswj kom txo qis cov nqi sib txuas ntxiv thiab nce kev nce qib. Kev npaj ntawm DNBSEQ cov tsev qiv ntawv suav nrog DNA fragmentation, kev npaj ntawm ssDNA, thiab dov lub voj voog kom tau txais DNA nanoballs (DNB). Cov no yog tom qab ntawd thauj mus rau qhov chaw ruaj khov thiab tom qab sib txuas los ntawm combinatorial Probe-Anchor Synthesis (cPAS). DNBSEQ thev naus laus zis sib xyaw ua ke qhov zoo ntawm qhov muaj qhov tsis zoo ntawm kev ua yuam kev tsawg nrog kev siv cov qauv kev ua yuam kev siab nrog nanoballs, uas ua rau muaj kev sib txuas nrog ntau dua thiab qhov tseeb.
Peb lub tsev qiv ntawv ua ntej ua ke ua ke ua rau cov neeg siv khoom npaj Illumina sequencing cov tsev qiv ntawv los ntawm ntau qhov chaw (mRNA, tag nrho cov genome, amplicon, 10x cov tsev qiv ntawv, thiab lwm yam), uas tau hloov mus rau MGI cov tsev qiv ntawv hauv peb lub chaw soj nstuam kom ua tiav hauv DNBSEQ-T7, ua rau cov ntaub ntawv siab ntawm cov nqi qis.
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Hi-C raws Chromatin Interaction
Hi-C yog ib txoj hauv kev tsim los ntes genomic configuration los ntawm kev sib txuas kev soj ntsuam kev sib thooj-raws li kev sib raug zoo thiab kev sib tw siab. Cov txheej txheem yog ua raws li chromatin crosslinking nrog formaldehyde, ua raws li kev zom zaub mov thiab rov ua dua tshiab nyob rau hauv ib txoj kev uas tsuas yog cov seem uas txuas nrog covalently yuav tsim cov khoom ligation. Los ntawm kev ua raws cov khoom lag luam ligation, nws tuaj yeem kawm txog 3D lub koom haum ntawm genome. Hi-C tso cai rau kev kawm txog kev faib tawm ntawm feem ntawm cov genome uas tau ntim me me (A compartments, euchromatin) thiab feem ntau yuav ua rau muaj kev cuam tshuam, thiab cov cheeb tsam uas ntim ntau dua (B compartments, Heterochromatin). Hi-C kuj tuaj yeem siv los txheeb xyuas Topologically Associated Domains (TADs), thaj chaw ntawm cov genome uas muaj cov qauv sib dhos thiab yuav muaj cov qauv qhia zoo sib xws, thiab txhawm rau txheeb xyuas cov chromatin loops, thaj tsam DNA uas tau koom ua ke los ntawm cov proteins thiab uas yog feem ntau enriched nyob rau hauv kev cai hais. BMKGene's Hi-C sequencing service pab txhawb cov kws tshawb fawb los tshawb txog qhov loj me ntawm genomics, qhib txoj hauv kev tshiab rau kev nkag siab txog kev tswj hwm genome thiab nws qhov cuam tshuam rau kev noj qab haus huv thiab kab mob.
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TGuide Ntse Sib Nqus Nroj Tsuag RNA Cov Khoom Siv
TGuide Ntse Sib Nqus Nroj Tsuag RNA Cov Khoom Siv
Purify zoo tag nrho RNA los ntawm cov ntaub so ntswg
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TGuide Smart Blood/Cell/Tissue RNA Kit
TGuide Smart Blood/Cell/Tissue RNA Kit
Cov khoom siv prefilled / phaj reagent rau purification ntawm high-yield, high-purity, high-zoo, inhibitor-free tag nrho RNA los ntawm cov tsiaj cov ntaub so ntswg / cell / tshiab tag nrho cov ntshav.
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TGuide Ntse Magnetic Plant DNA Kit
TGuide Ntse Magnetic Plant DNA Kit
Purify high-zoo genomic DNA los ntawm ntau yam nroj tsuag cov ntaub so ntswg
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TGuide Ntse Av / quav DNA Kit
TGuide Ntse Av / quav DNA Kit
Purifies inhibitor-dawb DNA ntawm siab purity thiab zoo los ntawm cov av thiab cov quav
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TGuide Ntse DNA Purification Kit
Rov qab zoo DNA los ntawm PCR cov khoom lossis agarose gels.
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TGuide Ntse Ntshav Genomic DNA Kit
TGuide Ntse Ntshav Genomic DNA Kit
Cov khoom siv prefilled / phaj reagent rau genomic DNA purification los ntawm cov ntshav thiab buffy tsho
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TGuide Ntse Magnetic Tissue DNA Kit
Cov khoom siv prefilled / phaj reagent rau genomic DNA rho tawm los ntawm cov ntaub so ntswg tsiaj
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TGuide Ntse Universal DNA Kit
Cov khoom siv prefilled / phaj reagent rau purification genomic DNA los ntawm cov ntshav, cov ntshav qhuav, kab mob, hlwb, qaub ncaug, qhov ncauj swabs, tsiaj cov ntaub so ntswg, thiab lwm yam.
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TGuide S16 Nucleic Acid Extractor
TGuide S16 Nucleic Acid Extractor
Ib qho yooj yim-rau-siv Benchtop Instrument, 1-8 Los yog 16 Cov Qauv Tib Lub Sijhawm
Catalog tooj / ntim
Cat. tsis muaj
ID
Tsis muaj preps
OSE-S16-AM
1set ib
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PacBio 2+3 Full-Length mRNA Solution
Thaum NGS-raws li mRNA sequencing yog ntau yam cuab yeej rau kev txheeb xyuas cov noob qhia, nws qhov kev cia siab ntawm kev nyeem luv luv txwv nws txoj kev ua tau zoo hauv kev txheeb xyuas cov ntaub ntawv transcriptomic. Ntawm qhov tod tes, PacBio sequencing (Iso-Seq) ntiav cov thev naus laus zis nyeem ntev, ua kom muaj kev sib luag ntawm cov ntawv sau ntev mRNA. Txoj hauv kev no ua rau muaj kev tshawb nrhiav dav dav ntawm lwm txoj kev sib txuas, cov noob fusions, thiab poly-adenylation, txawm hais tias nws tsis yog qhov kev xaiv tseem ceeb rau kev nthuav qhia cov noob. 2 + 3 kev sib xyaw ua ke txuas qhov sib txawv ntawm Illumina thiab PacBio los ntawm kev tso siab rau PacBio HiFi nyeem los txheeb xyuas qhov ua tiav ntawm cov ntawv teev lus isoforms thiab NGS sequencing kom ntsuas cov isoforms zoo ib yam.
Platforms: PacBio Sequel II / PacBio Revio thiab Illumina NovaSeq;
