Nroj / Tsiaj Tag Nrho Cov Secome Seag Zog
Cov Nta
● Kev npaj lub tsev yuav khoom tuaj yeem ua qauv lossis pcr-dawb
● Muaj nyob rau hauv 4 cov txheej txheem ua ntu zus: ease novaseq, mgi T7, Nanopore Promethion P48, lossis Pacbio Revio.
● Bioinformatic Kev tsom xam rau kev kuaj pom ntawm cov variants: SNP, Indel, SV thiab CNV
Kev Pabcuam Zoo
●Cov ntaub ntawv tshaj lij thiab cov ntaub ntawv tshaj tawm: Cov kev paub ntau heev hauv Genome Securencing rau ntau dua 1000 hom tau ua rau muaj cov kev qhia cuam tshuam ntau tshaj li ntawm 5000.
●Kev txheeb xyuas qhov twg?: Xws li kev hloov hu thiab ua haujlwm tshaj xov xwm.
● Cov muag khoom txhawb:Peb cov lus cog tseg txuas ntxiv nthuav tawm cov phiaj xwm ua tiav nrog 3-hli tom qab muag lub sijhawm pabcuam. During this time, we offer project follow-up, troubleshooting assistance, and Q&A sessions to address any queries related to the results.
●Kev qhia tshwj xeeb: Peb siv ntau yam databases kom muaj nuj nqi rau cov caj ces nrog cov kev sib txawv thiab ua rau pom muaj kev tshawb fawb txog kev tshawb fawb txog ntau yam.
Kev Pabcuam Tshwj Xeeb
| Cov variants kom raug txheeb xyuas | Seitencing Lub Tswv Yim | Pom zoo tob |
| SNP thiab Indel | Icina Novase zaum PE150 lossis mgi t7 | 10 x |
| SV thiab CNV (tsawg dua) | 30 X | |
| SV thiab CNV (muaj tseeb dua) | Nanopore Prom p48 | 20 x |
| SNPs, Indels, SV thiab CNV | Pacbio Revio | 10 x |
Qauv Yuav Tsum Tau
| Cov ntaub so ntswg lossis rho tawm nucleic acids | Illina / mgi | Tus pw | Pacbio
| ||
| Tsiaj Viscera | 0.5-1 g | ≥ 3.5 g
| ≥ 3.5 g
| ||
| Cov leeg tsiaj | ≥ 5 g
| ≥ 5 g
| |||
| Cov ntshav Mammalian | 1.5 ml | ≥ 0.5 ml
| ≥ 5 ml
| ||
| Nqaij qaib / Ntses Ntshav | ≥ 0.1 ML
| ≥ 0.5 ml
| |||
| Tsob ntoo- nplooj ntawv tshiab | 1-2 g | ≥ 2 g
| ≥ 5 g
| ||
| Cov cells |
| ≥ 1x107
| ≥ 1x108
| ||
| Cov ntaub so ntswg / tus neeg | 0.5-1 g | ≥ 1 g
| ≥ 3 g
| ||
| Rho DNA
| Concentration: ≥ 1 ng / μl Tus nqi: ≥ 30 ng Txwv tsis pub lossis tsis muaj kev nyuaj siab lossis paug
| Kev xav ntsoov Pes tsawg
OD260 / 280
OD260 / 230
Txwv tsis pub lossis tsis muaj kev nyuaj siab lossis paug
| ≥ 40 ng / μl 4 μg / ndlwg ntawm tes / qauv
1.7-2.2
≥1.5 | Kev xav ntsoov Pes tsawg
OD260 / 280
OD260 / 230
Txwv tsis pub lossis tsis muaj kev nyuaj siab lossis paug | ≥ 50 ng / μl 10 μg / ndlwg ntawm tes / qauv
1.7-2.2
1.8-2.5 |
| Kev Npaj Luam PC-Dawb Cov Ntawv Npaj Ua: Kev tsom siab≥ 40 ng / μl Tus nqi≥ 500 ng | |||||
Kev Pabcuam Khiav
Qauv Xa
DNA extraction
Kev Siv Hauv Tsev Qiv Ntawv
Ze
Cov Ntaub Ntawv Kev Ntsuam Xyuas
Cov Ntaub Ntawv Xa
Xws li kev tsom xam hauv qab no:
- Cov Ntaub Ntawv Nyoos Zoo Tswj
- Txheeb cais ntawm cov kev sib txawv los siv genome
- Kev txheeb xyuas tus kheej: SNP, Indel, SV thiab CNV
- Ua haujlwm tshaj tawm ntawm cov variants
Txheeb cais ntawm cov kev sib txawv los siv genome - sequelencing tob faib
SNP hu ntawm ntau cov qauv
Indel qhia txog tus kheej - kev txheeb cais ntawm Indel ntev hauv CDD cheeb tsam thiab thaj av Genome-dav
Kev sib txawv faib thoob plaws lub genome - circos zajlus
Ua haujlwm tshaj tawm ntawm cov noob uas tau txheeb xyuas cov sib txawv - gene oftology
Chai, Q. li al. (2023) 'ib lub glutathione s-hloov ghtt19 txiav txim paj popal pigmentation ntawm radulating antoschnient hauv paj rwb', cog biotechnology phau ntawv sau nkauj, 21 (2), p. 433. DOI: 10.1111 / PBI.13965.
Cheng, H. li al. (2023) 'Chromosome-qib Tsiaj Dub yog Genomic-Pab Cov Roj Hmab thiab cov ntawv xov xwm cog qoob loo tsim khoom, 21 (5), pp. 1058-1072. DOI: 10.1111 / PBI.14018.
Li, A. li al. (2021) 'Genome Ostuarine Oyster muab kev pom rau hauv kev nyab xeeb thiab kev sib xyaw ua ke', 1, 4 (1 (1 (1 (1 (pp. 1-12. DOI: 10.18 / s42003-021-02823-6.
Zeng, T. li al. (2022) Qhov kev hloov pauv Genome thiab methylation nyob rau hauv Suav cov qaib hauv paus txawm qhia txog kev txuag tsiaj, 5 (1), pp. 1-12. DOI: 10.18 / s42003-02907-7.
