MRNA-SEQ (NGS) nrog siv genome

MRNA-SEQ (NGS) nrog siv genome

RNA-SEQ yog tus qauv txheem thoob plaws lub neej thiab qoob loo sciences, bridging qhov sib txawv ntawm genomes thiab proteomes. Nws lub zog nyob hauv kev nrhiav cov ntawv sau tshiab thiab cov nuj nqis lawv cov lus qhia hauv ib qho kev xav. Nws tau dav siv rau kev sib piv cov kev sib piv ntawm cov caj ces muaj rau cov tsiaj qus lossis ua piv txwv rau cov tsiaj qus lossis pom cov noob uas piav qhia txog cov xwm txheej tshwj xeeb. BMKCLOUD MRNA (Siv) Kev Tshawb Fawb Kev Tshawb Fawb, Deg), thiab cov qauv kev sib txawv ntawm cov software zoo sib xws, ua kom yooj yim thiab cov neeg siv-ua phooj ywg. Cov neeg siv tau tso lawv cov ntaub ntawv RNA Ib qho ntxiv, nws ua ntej cov neeg siv khoom, muab cov haujlwm ntawm tus kheej ua rau cov neeg siv kev xav tau tshwj xeeb. Cov neeg siv tuaj yeem tsim cov kev txwv thiab xa cov raj xa hluav taws xob los ntawm lawv tus kheej, saib cov ntaub ntawv sib txuam, xws li: Kev xaiv clustering, kev ua raws li cov txheej txheem.

Demo Cov Ntsiab Lus

Cov ntaub ntawv tsuas

Ntshuam yuav tsum tau ua

Kev Tshawb Fawb

Phau ntawv

Demo Cov Ntsiab Lus

Cov ntaub ntawv tsuas

Ntshuam yuav tsum tau ua

Platform:Illina, mgi
Lub Tswv Yim:RNA-SEQ
Nthuav: Paried, ntxuav cov ntaub ntawv.
Hom tsev qiv ntawv:fr-unstranded, FR-thawjstrand lossis fr-vib nastrand
Nyeem ntev:150 bp
Cov hom ntaub ntawv:* .Fastb, * .fq, * .fastq.gz lossis * .fq.gz. Lub kaw lus yuavcia li Khub cov ntaub ntawv .Fastb raws li thier npe,Piv txwv li * _1.Fastb paired nrog * ._ 2.5fastq.
Tus naj npawb ntawm cov qauv:Tsis muaj kev txwv rau tus lejntawm cov qauv, tab sis lub sijhawm tsom xam yuav nce raws li tus naj npawb ntawmKuaj loj hlob.
Cov Ntaub Ntawv Pom Zoo:6g rau ib yam piv txwv

Kev Tshawb Fawb

Cov cuab yeej tseem ceeb thiab bioinformatic cov khoom ntawm MRNA-SEQ (siv)Cov raj xa hluav taws yog raws li hauv qab no:

1. Rawata zoo tswj:

• Tshem tawm cov khoom ua tau zoo, adapter ua ntu zus,lwm yam;

• Cov Cuab Yeej: Hauv-Tsev Tsim Kev Tshawb Nrhiav Haujlwm;

2. Cov ntaub ntawv ntawm cov ntaub ntawv rau qhov siv Genome:

• Ua raws li kev nyeem tau zoo nrog kev sib tw-paub txog kev tiv thaiv covSiv Genome.

• Cov Cuab Yeej:HERAT2, samtools

3. Cov Tsev Khaws Khoom Zoo:

• Muab kev ntsuas ntev ntev kev ntsuas, ua ntu zaum ua kom pom tseeb, thiab lwm yam;

• Cov Cuab Yeej:samtools;

4. Sequence Qauv Qauv:

• Lwm Yam Kev Tshawb Fawb Txog Kev Tshawb Xyuas Cov Lus Qhia, Cov Noob Tes Tsim Kho Kom Zoo,Cov kev twv ua ntej gene twv ua ntej, thiab lwm yam;

• Cov Cuab Yeej:Txoj ntaub ntawv, hnab muag gilcompare, Tus gatk,Lub hlawv, Tus kws txhais lus, thiabHmmer.

5. Kev Ntsuas Ntxiv Kev Ntsuas:

• DEG tshuaj ntsuam, kev txheeb xyuas kev sib raug zoo, ua haujlwmkev txhawb nqa;

•Ntau cov txiaj ntsig pom tau;

•RnrogTus siqeq, DESEQ2, ggplot2, Dexseq

Phau ntawv

1. Kim, DaEhwan li al. "Cov duab graph raws genome sib zog thiabgenotyping nrog hisat2 thiab herat-genotype. "Tus yeeb yamBiotechnology37 (2019): 907 - 915.

2. McKenna, Aaron li al. "Lub genome tsom xam cov cuab yeej: aMapreduce moj khaum rau kev txiav txim siab tom ntej no cov DNAextraCerving cov ntaub ntawv. "Kev Tshawb Nrhiav GEME20 9 (2010): 1297-303.

3. Lis, Heng et al. "Cov txheej txheem sib txawv / Daim Ntawv Qhia Hom thiabSamtools. "Bioinformformic25 (2009): 2078 - 2079.

4. Peralea, Mihaela li al. "Hlawstie ua kom tau zoo duaKev tsim kho ntawm cov transcriptome los ntawm RNN-SeQ nyeem. "Tus yeeb yamBiotechnology33 (2015): 290-295.

5. Hlub, Michael I. li al. "Qhov kev kwv yees kev kwv yees ntawm cov kev hloov pauv thiabdispersion rau RNA-SEQ cov ntaub ntawv nrog Deseq2. "GenomeKev kawm tsiaj15 (2014): n. pag.

6. Eddy, Sean R .. "nrawm profile HMM tshawb nrhiav."PLOS Kev Sib Koom Tes Biology7 (2011): n. pag.

MRNA-SEQ (NGS) nrog siv genome

MRNA-SEQ (NGS) nrog siv genome

Tau txais cov lus tsocai

Xa koj cov lus rau peb: