Genome Evolution
Piag dej
Lub keeb kwm kev hloov pauv thiab keeb kwm domestication ntawm goldfish (Carassius Auratus)
Pacbio | Agina | Bionan Genome Daim Ntawv Qhia | Hi-C Genome Sib Tham | Daim duab qhia caj ces | Gwas | RNA-SEQ
Cov Tseem Ceeb
1. Cov Genmoos Genome tau hloov kho nrog cov kev sib txoos zoo siab, Anchoring 95.75% ntawm cov cross rau hauv 50 pseudochromosomes (Scaffold N50 = 31.84 MB). Ob lub subgenomes tau tsis txaus ntseeg.
2.Txoj cheeb tsam ntawm kev xaiv cheb thaum lub sijhawm ua haujlwm tau txheeb xyuas los ntawm cov ntaub ntawv rov qab los ntawm 20190 cov noob neej muaj feem cuam tshuam nrog cov kev sib tw hauv kev teeb meem.
3.Gwas ntawm dorsal tis nyob rau hauv domactic formalic qhia 378 tus neeg los tsim los ntawm cov noob uas muaj feem cuam tshuam. Tus neeg sau xov xwm Tyrosine-protein Kinsein Kinase tau txheeb raws li kev sib tw ua rau muaj kev sib tw nrog kev hloov pauv
Qhov nyob nram qab
Kub (Carassius autatus) yog ib qho ntawm cov ntses ua liaj ua teb tshaj plaws, uas yog kev ua haujlwm ntawm cov crucian carpep chapn hauv lub teb chaws Suav thaum ub. Lawv tau hais los ntawm Charles Darwin li "dhau ib qho kev yuav luag tsis kawg ntawm cov xim, peb tau sib ntsib nrog cov qauv zoo tshaj plaws ntawm cov qauv". Ntau yam muaj ntau yam sib txawv thiab ntev keeb kwm ntawm domestication thiab kev yug me nyuam ua goldfish ua tau zoo genetic qauv system rau ntses physiology thiab kev hloov pauv.
Kev ua tiav
Tus ntses genome
JOint tshuaj ntsuam ntawm pacbio thiab Illina Pauv-end extra Electrens cov ntaub ntawv yields ua ntej 1.657 g drraft N50 = 474 KB). Bionano Optical Daim Ntawv Qhia tau tsim thiab kho cov limhiam rau hauv 1.73 GB hauv qhov loj me (kwv yees nce qib: 1.8 GB). Hi-c raws li sib dhos txuas ntxiv txhim kho scaffold n50 ntawm 606 kb txog 31.84 MB rau 315.75% (1.65 GB) taw qhia thiab xaj anchoring tus nqi. Cov Genome muaj 56,251 coding genes thiab 10,098 ntev tsis-cod ntawv sau. Ntxiv mus, 38 lub peev xwm centromeric thaj chaw tau kwv yees tawm ntawm 50 chromosomes.
Fig.1 Kub Ntses Genome
TWo cov txheej txheem ntshiab ntawm cov subgenomes tau txheeb xyuas hauv 50 tus ntses kub ntses uas tau ua dhau los ntawm kev teeb meem hybridization txheej thaum ub. Lub teeb ntawm cov chromosomes nrog cov feem ntau dua ntawm kev nyeem ua raws li cov ntses kub thiab lub Chra01 ~ A25), piv txwv li kev tseem nyob ntawm Stergenome B (ChrB01 ~ B25).
Domestication thiab xaiv tau cheb
ATag nrho ntawm 16 tus tsiaj qus hom Crucian carps thiab 185 sawv cev goldfish variants tau rov qab nrog qhov tob tob ntawm kwv yees li 12.5x, tsim tawm 4.3 terabases ntawm cov ntaub ntawv. Kev Tshawb Fawb Phylogenetic thiab PCA tau lees paub txog cov ntses goldfish thiab cov crucian tau muab faib ua ob cajdab.
LKev tsom xam kom ntawm sab saud muaj kev txwv tsis pub muaj ntawm cov pej xeem ntawm cov pejxeem caj dab thaum lub sij hawm xaiv goldfish. Ua kom muaj kev sib tw dhau los (π) los ntawm CRUCIA los ntawm CROLIAL CARK ntxiv rau Goldfish thiab qe goldfish qhia txog kev sib txawv ntawm kev sib txawv ntawm kev hloov pauv thaum lawv lub neej. 50 xaiv cheb cov cheeb tsam npog 25,2 MB thiab 946 noob tau txheeb xyuas los ntawm cov ntaub ntawv sawv cev (33 tus ntses kub thiab 16 tus ntoo khaub thuas). Nthuav kev tshawb fawb mus rau 201 cov tib neeg, 393 caj ces teev cov cheeb tsam ntawm ua tiav kev cheb. Cov noob no tau pom ntawm cov khoom muaj ntau yam tsawg, uas muaj feem cuam tshuam nrog phenotypes cuam tshuam nrog cov teeb meem loj hauv goldfish.
Fig.3 Genome-dav Domestication-Engleiator
Gwas ntawm cov goldfish
DOrsal Fin yog qhov tseem ceeb ua kom paub qhov txawv ntawm wen foldfish los ntawm qe ntses. Gwas ntawm dorsal fin ntawm 96 wen goldfish thiab 87 qe ntses qhia thoob plaws 13 qhov kev faib tawm tsis sib xws ntawm cov noob ntawm cov noob hauv lub subgenomes. Cov neeg sib tw no muaj cov txheej txheem biological muaj kev sib tw "," Transmemranrane Tsheb Loj Hlob "," Lub Cev Kev Loj Hlob ", thiab lwm yam.
Fig.4 GWAS ntawm dorsal tis rau hauv cov ntses goldfish
IN Lub gene encoding ib tyrosine-protein kinase tus neeg txais raug txheeb xyuas hauv ib tus neeg sib tw sib koom tes.
Fig.5 GWAS ntawm Pob tshab Cov Hluav Taws Xob Hluav Taws Xob
Phau ntawv
CHen D li al. Lub keeb kwm kev hloov pauv thiab keeb kwm domestication ntawm goldfish (Carassius autatus). Pnas (2020)
Txoj moo Lub hom phiaj ntawm kev sib qhia cov rooj plaub zoo kawg nkaus nrog kev ua tiav biomarker, ntes cov txuj ci kev tshawb fawb tshiab uas muaj txiaj ntsig zoo raws li kev kawm.
Lub Sijhawm Post: Jan-04-2022