Tag nrho genome rov qab
Kev soj ntsuam Genomics ntawm SARS-COV-2 uncovov ntom nsp1 dedetion variant uas modforon teb
Nanopore | Agina | Tag nrho genome rov qab | Metagenomics | RNA-SEQ | Neeg thuam
Biomarmer technologies muab kev txhawb nqa kev txhawb nqa ntawm cov qauv ua ntu zus hauv txoj kev kawm no.
Cov Tseem Ceeb
1.sars-COV-1 Genome Excensing thiab Kev Tshawb Xyuas Phylincetic txheeb xyuas 35 rov qab cov kev hloov pauv suav nrog 31 sneps thiab 4 indels thiab 4 indels.
2. npaj nrog 117 lub tsev kho mob phaenotypes pom muaj peev xwm
tseem ceeb sib.
Δ500-532 hauv NSP1 coding cheeb tsam txheeb ze nrog tus vaub kib qis
3.Kuv thiab ntshav ifn-β.
4.Viral cais nrog Δ500-532 mutation induce qis dua ifn-i
teb nyob rau hauv cov cell kis.
Kev tsim qauv tsim
Kev ua tiav
1. Covid-19 Epidemiological thiab Kev Tshawb Xyuas Genomic
Cov ntaub ntawv hauv tsev kho mob tau sau rau hauv lub xeev Sichuan, Suav hla lub sijhawm tshwm sim los ntawm 538 covid-19, 2020. Tag nrho 2020. Peev. Cov ntaub ntawv paub tseeb nyob rau hauv Sichuan nce kev nthuav dav, peaking ntawm lub ib hlis ntuj. 30th. Tsis tas li, cov ntaub ntawv txhawb nqa tias kev sib txawv ntawm kev sib raug zoo tuaj yeem yog qhov tseem ceeb hauv kev tiv thaiv tus kabmob kis.
Daim duab 1. Epidemiological Txoj Kev Kawm ntawm Kevis-19 hauv Sichuan xeev, Suav
2. SARS-COV-1 GENOME Kev Tsim Kho thiab Ntau Yam Cim
Nrog ntau cov ampification ua raws li los ntawm Nanopore exterencing, tag nrho ntawm 310 ze- lossis ib nrab-ua tiav genomes los ntawm 248 tus neeg mob tau tsim nrog kwv yees li ntawm. 80% ntawm genomes them los ntawm 10 nyeem (txhais tau li cas: 0.39 m nyeem ib qho qauv).
Daim Duab 2. Kev Zij Ntawm Txhua Qhov Txawv Hauv Lub Sichuan Cohort
Tag nrho ntawm 104 SnPs thiab 18 Isdels tau txheeb xyuas los ntawm SARS-COV-CAY-CE-2 GUPES, nyob rau hauv uas 13 snps tau txheeb raws li cov kev sawv rov los genetent. Los ntawm kev sib piv nrog 169 kuaj los ntawm Wuhan thiab nrog 81,391 Zoo tshaj tshaj tawm cov Gisaid, 29 ntawm 35 tus neeg sib txawv pom nyob rau lwm cov teb chaws. Tsis muaj tseeb, plaub qhov sib txawv xws li δ500-532, ACC1830, tsuas yog pom tias cov khoom siv gisaid tau pom los ntawm Wuhan, uas tau ntsib lub Cov ntaub ntawv taug kev ntawm cov neeg mob.
Kev soj ntsuam kev txheeb xyuas nrog qhov siab tshaj plaws (ml) thiab Bayesian molecular moos txoj kev sib cuag tau ua tiav tau ua tiav tau ua tiav thiab 250 tus txiv neej tshiab los ntawm lwm thaj chaw. Genomes nrog δ500-532 (kho kom haum rau hauv NSP1 Coding cheeb tsam) tau pom tias muaj sparsely sparsely nyob rau hauv cov ntoo phylogenetic. Kev txheeb xyuas Haplotype ntawm NSP1 cov neeg txawv tau txheeb txog 5 ntawm lawv los ntawm ntau lub nroog. Cov txiaj ntsig tau qhia tias lub δ500-532 tshwm sim hauv ntau lub nroog thiab tej zaum yuav yog imported ntau zaus los ntawm Wuhan.
Daim duab 2. Cov kev txheeb xyuas caj ces caj ces thiab cov phylogenetic hauv lub SARS-COV-2 GENOMES
3. Koom haum ntawm cov kev rov qab tshwm sim genetic variants nrog cov chaw kuaj mob cuam tshuam
117 Kho Mob Phenotypes tau cuam tshuam nrog covid-19 qhov mob hnyav, qhov twg 19 qhov hnyav tshaj plaws ntawm cov phenotypes tau muab cais rau hauv cov cwj pwm hnyav thiab tsis yog. Kev sib raug zoo ntawm cov kev sib raug zoo no thiab 35 cov kev rov ua dua tshiab genetic variants tau variized hauv bi-pawg hluav taws xob. Ib qho Gsea-li Banked Intrichment Tshawb xyuas pom tias δ500-532 yog tsis zoo nrog ESR, Ntshav Qab Zib Cell-β thiab Cell suav hauv cov ntshav. Ntxiv mus, QPRCTs qhia tau tias cov neeg mob harbored δ500-532 tau muaj nqi ntau tshaj plaws, piv txwv li tus kab mob qis tshaj plaws.
Daim duab 3. Koom Haum Xeeb ntawm 35 Cov Kev Recurent Genety Cov Variants nrog cov tshuaj ntsuam Penotypes
4. Kev siv tau rau ntawm kev sib txuas sib xyaw ua ke Phenotypes
Txhawm rau kom nkag siab txog qhov cuam tshuam ntawm δ500-532 rau NSP1 cov haujlwm, HEK239T cov cell tau mus rau tag nrho, wt nsp1 thiab mutant cov ntaub ntawv nrog decetions. Transcriptome profiles ntawm txhua kho hek239t hlwb tau ua rau PCA kev tsom xam sawv cev sib txawv thiab tau txawv ntawm WT NSP1. Cov noob uas tau raug ntiab tawm nyob rau hauv cov mutants feem ntau yog enriceynthety "," RibermedeSin), "RibermedeSin)," Ribermedination)
Daim duab 4. Kev Tshawb Fawb Kev Tshawb Xyuas ntawm Hek239T cov hlwb los ntawm WT NSP1 thiab uas nrog tshem tawm
Qhov cuam tshuam ntawm deletions ntawm ifn-1 teb kuj tau sim ua kev kawm dhau los ua. Txhua qhov kev ntsuas deletions tau tshwm sim los txo IFN-1 RepSSE hauv transfected Hek239t thiab A549 hlwb ntawm theem transcriptome thiab qib protein. Qhov zoo siab, qhov tseem ceeb-cov khoom siv tswj hwm tau ua rau tus kab mob "," kev tiv thaiv gennome "," kev tiv thaiv Genpome Genication "thiab" teb rau hom uas kuv Interferon ".
Daim duab 5. Down kev cai ntawm txoj kev cuam tshuam nrog txoj kev cuam tshuam hauv δ500-532 mutant
Hauv txoj kev kawm no, kev cuam tshuam ntawm cov kab mob no rau tus kab mob tau lees paub ntxiv los ntawm kev tshawb fawb tus kab mob kis tau. Cov kab mob uas qee cov mutants tau cais los ntawm cov kuaj mob thiab kis rau Calu-3 hlwb. Cov ncauj lus kom ntxaws ntawm kev kis tus kab mob kis tau tuaj yeem nyeem hauv daim ntawv.
Doi:10.1016 / J.CHOM.202.01.015
Phau ntawv
Lin J, Tang C, Wei H, li al. Kev soj ntsuam genomic ntawm SARS-COV-2 uncovov ib tug nsp1 dedetion variant uas modferon teb [J]. Tus Thawj Coj ntawm tes & Micrebe, 2021.
Xov xwm thiab cov ntsiab lus tseem ceeb Lub hom phiaj ntawm kev sib qhia cov rooj plaub zoo kawg nkaus nrog kev ua tiav biomarker, ntes cov txuj ci kev tshawb fawb tshiab uas muaj txiaj ntsig zoo raws li kev kawm.
Lub Sijhawm Post: Jan-06-2022