Nanopore Daim Ntawv Qhia Tag Nrho

Nanopore transcriptome sequencing yog ib txoj hauv kev zoo rau kev ua kom tiav cDNAs tag nrho, txheeb xyuas kom raug thiab ntsuas cov ntaub ntawv isoforms. BMKCloud Nanopore Full-Length Transcriptome Pipeline yog tsim los tshuaj xyuas RNA-Seq cov ntaub ntawv tsim tawm ntawm Nanopore platform tiv thaiv cov khoom siv tau zoo tshaj plaws, muab cov txiaj ntsig zoo thiab kev tshuaj xyuas ntau ntawm ob theem noob thiab cov ntawv sau tseg. Tom qab kev tswj hwm zoo, tag nrho-ntev tsis-chimeric (FLNC) ib ntus tau txais thiab cov kev pom zoo ua ntu zus tau ua rau cov ntaub ntawv siv genome kom tshem tawm cov ntawv sau tsis raug. Los ntawm cov ntawv teev lus no, cov lus qhia muaj ntau thiab sib txawv ntawm cov noob caj noob ces thiab cov ntawv sau tseg raug txheeb xyuas thiab ua haujlwm tau piav qhia. Cov raj xa dej kuj suav nrog lwm qhov kev tshuaj xyuas polyadenylation (APA), kev tshuaj xyuas lwm txoj kev sib txuas, kev soj ntsuam yooj yim rov ua dua (SSR), kev kwv yees ntawm lncRNA thiab cov hom phiaj sib xws, kev kwv yees ntawm coding sequences (CDS), gene family analysis, transcription factor analysis, prediction of new genes. thiab functional annotation ntawm transcripts.