mRNA-seq (NGS) – De novo

mRNA sequencing tso cai rau profiling ntawm tag nrho cov mRNA transcripts nyob rau hauv lub hlwb nyob rau hauv tej yam kev mob thiab yog ib tug dav siv technology nyob rau hauv ntau yam kev tshawb fawb. BMKCloud De novo mRNA-seq pipeline yog tsim los tshuaj xyuas poly-A enriched sequencing cov tsev qiv ntawv thaum tsis muaj genome siv. Lub raj xa dej pib nrog kev tswj kom zoo, ua raws lide novtranscript sib dhos thiab unigene teeb xaiv. Lub unigene qauv tsom xam kwv yees coding sequence (CDS) thiab yooj yim ib theem zuj zus (SSR). Tom qab ntawd, kev tshuaj xyuas qhov sib txawv ntawm qhov sib txawv pom pom qhov sib txawv ntawm cov noob (DEGs) ntawm cov kev sim ntsuas, ua raws li cov lus piav qhia ua haujlwm thiab ua kom muaj txiaj ntsig ntawm DEGs kom tshem tawm cov kev xav lom neeg.