Hi-c raws li genome sib dhos
Hi-C yog ib txoj kev tsim los ntes chromosome kev teeb tsa los ntawm kev sib txuas ua ke kev sib cuam tshuam thiab kev sib txuas siab ntawm kev sib txuas lus siab. Kev siv ntawm cov kev sib cuam tshuam no ntseeg tau tias tsis zoo nrog kev sib haum xeeb nrog lub cev nrug ntawm chromosomes. Yog li ntawd, hi-c cov ntaub ntawv yog siv los coj cov clustering, kev txiav txim, thiab kev sib sau ua ke hauv cov cua ntsawj ntshab thiab anchoring cov uas nyob rau hauv ib tug naj npawb ntawm cov chromosomes. Cov cuab yeej no ua rau lub Chromosome-qib Genome sib dhos hauv qhov tsis muaj cov pejxeem ntawm cov pejxeem daim ntawv qhia txog caj ces. Txhua tus granome xav tau ib hi-c.
Cov Nta
● Kev sib txuas ntawm Illina Novase nrog PIB150.
● Kev pabcuam yuav tsum muaj cov ntaub so ntswg, tsis txhob muab cov kua qaub extracted, kom hla nrog formaldehyde thiab tsom kom cov DNA-protein kev sib cuam tshuam.
● Cov kev sim hi-c sim suav nrog kev txwv thiab xaus kho ntawm cov nplaum xaus nrog biotin, ua raws li kev sib tw ntawm kev sib tw blasks thaum khaws cov kev sib cuam tshuam. Cov DNA yog tom qab rub tawm nrog Streptavidir hlaws thiab ua kom huv rau kev npaj tom tsev txuas ntxiv.
Kev Pabcuam Zoo
Txheej txheem cej luam ntawm hi-c
(Lierman-Aiden e li al.,Kev kawm txuj ci, 2009)
●Tshem tawm qhov kev xav tau rau cov khoom caj ces cov ntaub ntawv:Hi-C hloov cov ntaub ntawv tseem ceeb uas xav tau rau Contig Anchoring.
●Siab cim ntom ntom:Ua rau lub siab contig anchoring piv rau saum 90%.
●Kev txawj ntse thiab cov ntaub ntawv tshaj tawm:BMKGAE muaj kev paub ntau ntau dua 2000 tus neeg mob ntawm Hi-C Genome ua ke los ntawm 1000 ntau hom thiab ntau hom patents. Tshaj 200 tus neeg tshaj tawm muaj kev cuam tshuam nrog ntau qhov cuam tshuam ntawm ntau dua 2000.
●Cov kws txawj ntse heev BioinformNrog rau hauv-tsev patents thiab software catenights rau hi-c kev sim thiab cov ntaub ntawv ntsuas kev pom kev software, thim rov qab, thim rov qab, thiab rov ua dua.
●Kev txhawb nqa tom qab:Peb cov lus cog tseg txuas ntxiv nthuav tawm cov phiaj xwm ua tiav nrog 3-hli tom qab muag lub sijhawm pabcuam. During this time, we offer project follow-up, troubleshooting assistance, and Q&A sessions to address any queries related to the results.
●Kev qhia tshwj xeeb: Peb siv ntau yam databases kom muaj nuj nqi rau cov caj ces nrog cov kev sib txawv thiab ua rau pom muaj kev tshawb fawb txog kev tshawb fawb txog ntau yam.
Kev Pabcuam Tshwj Xeeb
| Kev npaj lub tsev qiv ntawv | Seitencing Lub Tswv Yim | Pom zoo cov ntaub ntawv tso zis | Kev Tswj Xyuas Zoo |
| Hi-C Library | Icina Novase zaum PE150 | 100 X | Q30 ≥ 85% |
Qauv Yuav Tsum Tau
| Nqaij | Yuav Tsum Tau |
| Tsiaj Viscera | ≥ 2 g |
| Cov leeg tsiaj | |
| Cov ntshav Mammalian | ≥ 2 ML |
| Nqaij qaib / Ntses Ntshav | |
| Tsob ntoo- nplooj ntawv tshiab | ≥ 3 g |
| Cov cells | ≥ 1x107 |
| Tus kab | ≥ 2 g |
Kev Pabcuam Khiav
Kev Tshawb Fawb Kev Tshawb Fawb
Qauv Xa
Kev Siv Hauv Tsev Qiv Ntawv
Ze
Cov Ntaub Ntawv Kev Ntsuam Xyuas
Cov Kev Pab Tom Qab Muag
1) Cov Ntaub Ntawv LAW QC
2) Hied Qiv QC: Kev kwv yees ntawm siv tau Hi-C Kev Sib Tham
3) Hi-c Assembly: Clustering ntawm cov sib txuas ua pab pawg, ua raws li kev txiav txim cais nyob hauv txhua pab pawg thiab muab cov kev qhia sib txuas
4) Hi-C Kev Ntsuam Xyuas
Hi-C Liv Qc Tsev QC - Kev kwv yees ntawm Hi-C Siv Tau Cov khub
Hi-C Assuaj - Txheeb cais
Cov kev ntsuam xyuas tom qab sib dhos - ua kom puam muaj teeb meem ntawm kev siv hluav taws xob ntawm cov khoom noj
Tshawb cov kev nce qib uas yooj yim los ntawm BMKGene's Hi-c los ntawm kev sib sau los ntawm kev sau cov ntaub ntawv curated.
TIAN, T. LI AL. (2023) 'Genetic sib dhos thiab kev ua kom muaj caj ces ntawm cov chaw nkaum uas txaus ntshai tiv thaiv kev kub ntxhov-tiv thaiv bateplasm', xwm genetics 2023 55: 3), pp. 496-506. DOI: 10.18 / s41588-023-01297-y.
Wang, Zl et al. (2020) 'ib qho kev sib dhos chromosome-standbly-stainbee ntawm Asian zib ntab APIs Cerana Gerana', Frontiers hauv Genetics, 11, p. 52414. DOI: 10.3389 / FGAENE.2020.00279 / BIBTEX.
Zhang, F. li al. (2023) 'qhia kev hloov pauv ntawm tropane alkaloid bihoynthes los ntawm kev tshuaj xyuas ob lub siab rau hauv Solanaceae tsev neeg', kev sib txuas lus 2023 14: 1, 4 (1). DOI: 10.18 / s41467-023-37133-4.
Zhang, X. li al. (2020) 'Cov ntoo ntawm Banyan thiab Pollinator Wasp muab kev nkag siab rau Fig-wasp coevolution', 183 (4), pp. 875-889.e17. DOI: 10.1016 / J.CEL.2020.09.043
