Hi-c raws li chromatin kev sib cuam tshuam
Cov Nta
● Kev sib txuas ntawm Illina Novase nrog PIB150.
● Kev pabcuam yuav tsum muaj cov ntaub so ntswg, tsis txhob muab cov kua qaub extracted, kom hla nrog formaldehyde thiab tsom kom cov DNA-protein kev sib cuam tshuam.
● Cov kev sim hi-c sim suav nrog kev txwv thiab xaus kho ntawm cov nplaum xaus nrog biotin, ua raws li kev sib tw ntawm kev sib tw blasks thaum khaws cov kev sib cuam tshuam. Cov DNA yog tom qab rub tawm nrog Streptavidir hlaws thiab ua kom huv rau kev npaj tom tsev txuas ntxiv.
Kev Pabcuam Zoo
●Qhov Zoo Tshaj Plaws Cov Qauv Cuam Tshuam: Txhawm rau kom muaj kev kub siab-c ua tau zoo ntawm cov hom sib txawv nrog nce mus txog 93% siv tau kev sib cuam tshuam khub.
●Kev txawj ntse thiab cov ntaub ntawv tshaj tawm:BMKGAE muaj kev paub loj heev nrog> 2000-C Cov phiaj xwm ua ntu zus los ntawm 800 hom sib txawv thiab ntau hom patents. Tshaj 100 tshaj tawm cov ntaub ntawv nrog kev sib txuam sib cuam tshuam ntawm ntau dua 900.
●Heev-txawj Bioinformformics Pab Pawg:Nrog rau hauv-tsev patents thiab software cenports rau hi-c kev sim thiab cov ntaub ntawv tsom xam thiab cov ntaub ntawv pom kev software tus kheej.
●Kev txhawb nqa tom qab:Peb cov lus cog tseg txuas ntxiv nthuav tawm cov phiaj xwm ua tiav nrog 3-hli tom qab muag lub sijhawm pabcuam. During this time, we offer project follow-up, troubleshooting assistance, and Q&A sessions to address any queries related to the results.
●Kev qhia tshwj xeeb: Peb siv ntau yam databases kom muaj nuj nqi rau cov caj ces nrog cov kev sib txawv thiab ua rau pom muaj kev tshawb fawb txog kev tshawb fawb txog ntau yam.
Kev Pabcuam Tshwj Xeeb
| Lub tsev qiv ntawv | Seitencing Lub Tswv Yim | Pom zoo cov ntaub ntawv tso zis | Hi-C teeb liab daws teeb meem |
| Hi-C Library | ICATINA PE150 | Chromatin Loop: 150x Tad: 50x | Chromatin voj: 10kb Tad: 40kb |
Yuav Tsum Tau Ua Haujlwm
| Qauv Hom | Yuav Tsum Tau |
| Tsiaj Cov Ntaub So | ≥2g |
| Tag nrho cov ntshav | ≥2ml |
| Nceb | ≥1G |
| Cog- cov nqaij mos | 1g / aliquot, 2-4 aliquotes pom zoo |
| Cov cells | ≥1X107 |
Xws li kev tsom xam hauv qab no:
● Cov ntaub ntawv nyoos QC;
● Mw Fitel LCNA QC: Siv tau kev cuam tshuam khub thiab cuam tshuam ntawm kev khaws tseg (IDES);
● Genome-dav kev sib cuam tshuam profilation: CIS / CL kev tshawb xyuas thiab Hi-C sib tham hauv daim ntawv qhia;
● ● ● ● ● Kev Ntsuam Xyuas ntawm A / B ComputerThaum ntawd faib tawm;
● Kev txheeb xyuas ntawm TADS thiab Chromatin loops;
● Kev xam phaj txawv ntawm 3D Chratin cov qauv elements ntawm cov qauv thiab kev sib raug zoo tshaj tawm cov khoom siv txuam.
CIS thiab hloov cov nqi faib tawm
Kev tshav kub tshav ntawm cov kev sib txuam ntawm cov qauv sib cuam tshuam ntawm cov qauv
Genome-dav faib ntawm A / B Compartments
Genom-dav faib ntawm cov lipomatin loops
Kev pom ntawm Tads
Tshawb cov kev tshawb fawb txog kev tshawb fawb los ntawm BMKGene's Hi-C sib txuas cov kev pabcuam los ntawm kev sau cov ntawv sau ntawm cov ntawv tshaj tawm.
Meng, T. li al. (2021) 'Kev sib piv kev sib xyaw ua ke omics tsom xam cov cim CA2 raws li lub hom phiaj tshiab rau chordoma',Neuro-oncology, 23 (10), pp. 1709-1722. DOI: 10.1093 / Neuonc / Noab156.
Xu, L. li al. (2021) '3D kev tsis sib haum xeeb thiab kev rov ua pov thawj los ntawm pathogenesis ntawm nafld los ntawm kev sib xyaw Hi-C, Nanopore, thiab RNNing expressioning',Acta Pharmautica Sinica B, 11 (10), pp. 3150-3164. DOI: 10.1016 / J.PREB.202.032.
