De Novo fungal genome sib dhos
BMKGENES Muaj cov kev daws teeb meem ntau yam rau fungal menomes, catering rau ntau cov kev tshawb fawb xav tau thiab xav tau genome ua tiav. Siv kev ua ntu zus Cov lus luv luv thiab ntev-nyeem cov kev siv ua ntu zus uas siv cov nanopore lossis pacbio tau ua ke rau ntau lub fungal gorgal genome nrog cov contigs ntev dua. Ntxiv mus, ua ntu zus-C sib txuas ntxiv txhim kho lub peev xwm, ua kom muaj kev ua tiav ntawm kev ua tiav chromosome-qib genome.
Cov Nta
Nrog peb txoj kev xaiv los xaiv los ntawm nyob ntawm qhov xav tau degree ntawm genome ua tiav:
● cua ntsawj xaiv: Gentry xaiv: Kev sib txuas lus luv luv nrog Illina Novasei PE150.
Fungal Nplua genrome kev xaiv:
Daim ntawv ntsuam xyuas genome: Icmina Novase zaum PE150.
Genome Sib Dhos: Pacbio Revio (HiFi nyeem) lossis Nanopore Provethion 48.
● Chromosome-qib fungal genome:
Daim ntawv ntsuam xyuas genome: Icmina Novase zaum PE150.
Genome Sib Dhos: Pacbio Revio (HiFi nyeem) lossis Nanopore Provethion 48.
Contell anchoring nrog Hi-C Asfullly.
Kev Pabcuam Zoo
●Ntau txoj kev ua ntu zus muaj: Rau cov hom phiaj sib txawv thiab cov cai xav tau ntawm qhov ua tiav Genome
●Ua tiav Bioinformformics ua haujlwm:Qhov no suav nrog Genome sib dhos thiab cov twv ua ntej ntawm ntau cov ntsiab lus genomic, cov noob ua haujlwm ntxiv, thiab contig anchoring.
●Kev txawj ntse dav: Nrog ntau tshaj 12,000 microbial genomes sib sau ua ke, peb nqa dhau kev paub dhau los, cov ntsiab lus tsom xam, thiab cov kev muag khoom tom qab zoo nkauj.
●Kev txhawb nqa tom qab:Peb cov lus cog tseg txuas ntxiv nthuav tawm cov phiaj xwm ua tiav nrog 3-hli tom qab muag lub sijhawm pabcuam. During this time, we offer project follow-up, troubleshooting assistance, and Q&A sessions to address any queries related to the results.
Kev Pabcuam Tshwj Xeeb
| Kev pab | Seitencing Lub Tswv Yim | Kev Tswj Xyuas Zoo |
| Tawv genome | ICATINA PE150 100 X | Q30-85% |
| Zoo genome | Daim Ntawv Qhia Genu: Illina PE150 50 x Los ua ke: Pacbio HiFi 30x lossis nanopore 100 x | Contig n50 ≥1MB (Pacbio Unicellular) Contig n50 ≥2MB (ont unicellular) Contig n50 ≥500kb (lwm tus) |
| Chromosome-theem genome | Daim Ntawv Qhia Genu: Illina PE150 50 x Los ua ke: Pacbio HiFi 30x lossis nanopore 100 x Hi-c los ua ke 100x | Contig Anchoring piv> 90%
|
Yuav Tsum Tau Ua Haujlwm
| Concentration (ng / μl) | Tag nrho cov nyiaj (μg) | Ntim (μl) | OD260 / 280 | OD260 / 230 | |
| Pacbio | ≥20 | ≥2 | ≥20 | 1.7-2.2 | ≥1.6 |
| Tus pw | ≥40 | ≥2 | ≥20 | 1.7-2.2 | 1.0-3.0 |
| Tsom iav | ≥1 | ≥0.06 | ≥20 | - | - |
Unicellular fungus: ≥3.5x1010 ig
Macro fungus: ≥10 g
Kev Pabcuam Khiav
Qauv Xa
Kev Siv Hauv Tsev Qiv Ntawv
Ze
Cov Ntaub Ntawv Kev Ntsuam Xyuas
Cov Kev Pab Tom Qab Muag
Xws li kev tsom xam hauv qab no:
Daim Ntawv Nug Genome:
- Ua Ntej Cov Ntaub Ntawv Tswjhwm Zoo
- Genome kwv yees: Qhov loj me, heterozygosity, rov ua dua
Genome Genome Sib Dhos:
- Ua Ntej Cov Ntaub Ntawv Tswjhwm Zoo
- De novoRooj sib sau
- Kev Tshawb Xyuas GEME tiv thaiv: twv ua ntej ntawm CDS thiab ntau yam genomic
- Kev ua haujlwm tshaj tawm nrog ntau cov ntaub ntawv dav dav (mus, Kgg, thiab lwm yam) thiab Ady tau Databases (daim npav, VFDB, thiab lwm yam)
Hi-C Asfbly:
- Kev soj ntsuam hi-C qiv ntawv.
- Contigs anchoring clustering, xaj thiab kev sib koom tes
- Kev ntsuam xyuas ntawm Hi-C Assemly: Raws li cov qauv genome thiab cov cua sov
Daim Ntawv Ntsuam Xyuas Genu: K-Mer Faib
Genome sib dhos: Gene homologous andN database)
Genome sib dhos: ua haujlwm gene annotation (mus)
Tshawb nrhiav kev nce qib los ntawm BMKgene lub fungal genome ua ke cov kev pab los ntawm kev sau cov ntaub ntawv zoo nkauj.
Hao, J. li al. (2023) 'Kev sib xyaw ua ke ntawm cov tshuaj ntsuab ncebBMC Genomics, 24 (1), pp. 1-12. DOI: 10.1186 / s12864-023-09656-Z / Cov nuj nqis / 3.
Lu, L. li al. (2023) 'Kev ua tiav qib siab qhia tawm cov kev hloov pauv thiab cov kab ke ntawm cov nplej ntse eyogp pathogen Rhizoctonia cerealis',Cov qoob loo Jop, 11 (2), pp. 405-416. DOI: 10.1016 / J.CJ.2022.07.024.
Zhang, H. li al. (2023) 'Cov peev txheej Genwb rau plaub scaraceedia ua rau muaj cov khoom siv nyiaj daus las ntawm cov muaj ntau lub turfgrasses',Kab Mob Nroj, 107 (3), pp. 929-934. DOI: 10.1094 / PDIS-08-22-1921-A
Zhang, SS thiab al. (2023) Cov pov thawj caj ces thiab molecular pov thawj ntawm tetrapolar mating system nyob rau hauv eule nceb Grifosa ',Phau ntawv Journal ntawm Fungi, 9 (10), p. 959. DOI: 10.3390 / JOF9100959 / S1.
