Evolutionary Genetics
Kev Pabcuam Zoo
Takagi li al.,Cov phau ntawv xov xwm cog, 2013
●Kev tsom xam Bioinformatic Bioinformatic:Kev ua kom muaj kev kwv yees kev kwv yees kev sib txawv, uas cuam tshuam txog kev hloov pauv ntawm hom uas muaj kev cuam tshuam nrog cov tsiaj sib luag
●Xaiv tau customized tsom xam: Xws li kev kwv yees ntawm divergence sij hawm thiab ceev raws li variations ntawm nucleotide thiab amino acids theem.
●Cov ntaub ntawv tshaj lij thiab cov ntaub ntawv tshaj tawm: Bmkgene tau sau ntau dhau ntawm cov pej xeem thiab cov phiaj xwm evoluted rau ntau dua 1000 hom kev sib txuas lus, cov nroj tsuag molecular, thiab lwm yam.
● Cov neeg txawj ntse heev Bioinformformics thiab kev tsom xam luv luv: Nrog rau kev paub zoo hauv kev nce qib siab tiag tiag, BMKGene pawg neeg tshem tawm kev txheeb xyuas nrog lub sijhawm nrawm nrawm.
● Cov muag khoom txhawb:Peb cov lus cog tseg txuas ntxiv nthuav tawm cov phiaj xwm ua tiav nrog 3-hli tom qab muag lub sijhawm pabcuam. During this time, we offer project follow-up, troubleshooting assistance, and Q&A sessions to address any queries related to the results.
Kev Pab Tshwj Xeeb thiab cov tseev kom muaj
| Hom ntu ntu | Pom zoo cov pej xeem | Seitencing Lub Tswv Yim | Nucleotide yuav tsum tau ua |
| Tag nrho guarome seals | ≥ 30 cov tib neeg, nrog ≥ 10 tus neeg los ntawm txhua pawg
| 10 x | Concentration: ≥ 1 ng / μl Tag nrho tus nqi≥ 30ng Txwv tsis pub lossis tsis muaj kev nyuaj siab lossis paug |
| Tseeb-Locus Fragment (SLF) | TAG SEAR: 10 x Tus naj npawb ntawm cov cim npe: <400 MB: WGS raug pom zoo <1GB: 100k cim npe 1G > 2GB: 300k cim npe Max 500k cim npe | Kev xav ≥ 5 ng / μl Tag nrho cov nqi ≥ li 80 ng Nanodrop Od260 / 280 = 1.6-2.5 Agarose gel: tsis muaj lossis txwv tsis zoo lossis paug
|
Kev Pabcuam Khiav
Kev Tshawb Fawb Kev Tshawb Fawb
Qauv Xa
Kev Siv Hauv Tsev Qiv Ntawv
Ze
Cov Ntaub Ntawv Kev Ntsuam Xyuas
Cov Kev Pab Tom Qab Muag
Cov kev pabcuam uas muaj kev soj ntsuam cov pej xeem cov qauv (pca, cov pejxeem stratification), cov pejxeem kev tsis txaus siab, xaiv cov kev sib txuas-kev xaiv ntawm cov chaw zoo). Cov kev pabcuam tseem tuaj yeem suav nrog kev txheeb xyuas customized (piv txwv li lub sijhawm divergence, gene flow).
*Demo cov txiaj ntsig tau qhia ntawm no yog txhua yam los ntawm genomes luam tawm nrog bmkgene
1.Yojolution tsom xam muaj kev tsim kho cov tsiaj phylogenetic, cov pejxeem qauv thiab PCA raws cov kev sib txawv ntawm caj ces.
Cov ntoo phylogenetic piv txog kev sib raug zoo se thiab evolutionary kev sib raug zoo ntawm hom poj zeej.
PCA lub hom phiaj rau kev pom tseeb ntawm cov neeg sab nraud.
Cov neeg muaj neeg nyob hauv cov pej xeem pom muaj kev sib txawv ntawm cov pej xeem sau hauv cov nqe lus ntawm txhua zaus ntau zaus.
Chen, thiab. Al.,Piag dej, 2020
2.selective cheb
Xaiv cheb yog hais txog tus txheej txheem uas ib qho txiaj ntsig tau xaiv thiab ntau zaus ntawm cov chaw tsis muaj zog tau nce ntxiv, ua rau muaj kev txo ntawm cheeb tsam.
Genome-dav nrhiav kev xaiv sweep thaj tsam yog ua los ntawm kev laij cov neeg muaj tus cwj pwm ntawm caj ces hauv ib kauj ruam (100 kb).
Nucleotide ntau haiv neeg (π)
Tajima d
Txhim kho qhov ntsuas (FST)
WU, thiab. Al.,Molecular Tsob Nroj, 2018
3.GET ntws
WU, thiab. Al.,Molecular Tsob Nroj, 2018
4.Demogrographic Keeb Kwm
Zhang, thiab. Al.,Xwm Ecology & Evolution, 2021
5.Divengence Time
Zhang, thiab. Al.,Xwm Ecology & Evolution, 2021
Tshawb cov kev nce qib uas yooj yim los ntawm BMKGene lub noob caj noob ces cov kev pabcuam los ntawm kev sau cov ntawv tshaj tawm:
Hassanyar, AK li al. (2023) 'Qhov kev pom zoo ntawm SNP molecular cov cim thiab cov neeg sib tw genees ntawm supbrood tus kab mob tsis kam nyob rau hauv APIs Cerana Larvae los ntawm tag nrho cov genome rov ua dua',Cov Ntaub Ntawv Thoob Ntiaj Teb ntawm Molecular Sciences, 24 (7). DOI: 10.3390 / iJMS24076238.
Chai, J. li al. (2022) Qhov kev tshawb pom ntawm cov tsiaj qus, caj ces cov huab tais dawb loj loj loj heev tsim cov kev txuag tshiab ',Kev Tshawb Fawb Zoological, 2022, vol. 43, Qhov 3, phab: 469-480, 43 (3), pp. 469-480. DOI: 10.24272 / J.issn.2095-8137.2022.101.
HAN, M. li al. (2022) 'qauv pyllogeographical qauv thiab cov pej xeem evolution illmus txiiricus L. Rau Qinghai-Tibetan Plateau',Frontiever nyob rau hauv cog science, 13, p. 882601. DOI: 10.3389 / FLSSS022.882601 / BIBTEX.
Wang, J. li al. (2022) 'Genomic kev pom rau hauv cov kev hloov kho ntev los ntawm ib tug Chromosome-qib Genome sib dhos thiab cov pejxeem Genomics ntawm Longan Supreed',Kev Tshawb Fawb Teb Ua Teb, 9. DOI: 10.1093 / hr / tuhac021.
