DNBSEQ ua ntej-ua tsev qiv ntawv
Nta
●Platform:Mgi-dnbseq-t7
●Summing hom:PE150
●Hloov chaw ntawm lub teeb taws teeb rauMgi:Kev ua kom muaj kev sib txuas ntawm cov ntaub ntawv siab ntawm tus nqi qis.
●Kev tswj hwm zoo ntawm cov tsev qiv ntawv ua ntej ua ntu zus.
●Cov ntaub ntawv QC thiab kev xa khoom:Kev xa cov QC daim ntawv qhia thiab cov ntaub ntawv nyoos hauv cov hom ceev tom qab demultipleXing thiab lim Q30 nyeem.
Qhov Zoo
●Versatility ntawm seals cov kev pab:Cov qhua tuaj noj mov tuaj yeem xaiv ua ntu zus los ntawm txoj kab lossis cov ntaub ntawv.
●Cov ntaub ntawv ntau:1500 GB / LANE
●Kev Xa Tawm ntawm Sequencing QC qhia:Nrog kev ntsuas zoo, cov ntaub ntawv raug thiab tag nrho kev ua tau zoo ntawm kev ua ntu zus.
●Cov Txheej Txheem Elixed:nrog luv tig-ncig.
●Kev tswj kom nruj: Peb siv nruj QC cov kev xav tau kom lav tau tias kev xa cov txiaj ntsig zoo hauv cov txiaj ntsig zoo.
Qauv Yuav Tsum Tau
| Cov ntaub ntawv tus nqi (x) | Concentration (QPCR / NM) | Qho loj hnyav | |
| Ib nrab kab
| X ≤ 10 GB | ≥ 1nm | ≥ 25 μl |
| 10 GB <X ≤ 50 GB | ≥ 2 nm | ≥ 25 μl | |
| 50 gb <X ≤ 100 GB | ≥ 3 nm | ≥ 25 μl | |
| X> 100 gb | ≥ 4 nm | ||
| Ib txoj kab | Ib txoj kab | ≥ 1.5 NM / FARDED FARDON | ≥ 25 μl / Tsev qiv Ntawv Pas |
Ntxiv rau kev xav thiab tag nrho cov nyiaj, ib qho qauv peak zoo kuj tseem xav tau.
Nco tseg: Txoj kab kev sib txuas ntawm cov tsev qiv ntawv muaj ntau haiv neeg yuav tsum phix taws-hauv kom muaj kev hu kom ruaj khov.
Peb pom zoo xa cov tsev qiv ua ntej ua ntej. Yog tias koj xav tau BMKgene los ua cov tsev qiv ntawv pas, thov saib mus rau
Cov tsev qiv ntawv yuav tsum muaj rau ib nrab kab sib txuas.
Lub tsev qiv ntawv loj (peak daim ntawv qhia)
Lub ntsiab tseem ceeb yuav tsum tsis pub dhau 300-450 bp.
Cov tsev qiv ntawv yuav tsum muaj ib lub ncov loj, tsis muaj kev sib kis thiab tsis muaj qhov chaw tso quav.
Kev Pabcuam Workflow
Daim Ntawv Qhia QC Daim Ntawv Qhia
Daim ntawv tshaj tawm nyob rau hauv qhov zoo ntawm lub tsev qiv ntawv tau muab ua ntej ua ntu zus, ntsuas chaw qiv ntawv, thiab tawg.
Txuas QC Daim Ntawv Qhia
Cov lus 1. Txheeb cais ntawm cov ntaub ntawv sib txuas.
| Qauv ID | BMKID | RAW tau nyeem | Cov ntaub ntawv nyoos (Bp) | Cov nyeem kom huv (%) | Q20 (%) | Q30 (%) | GC (%) |
| C_01 | BMK_01 | 22,870,120 | 6,861,036,000 | 96.48 | 99.14 | 94.85 | 36.67 |
| C_02 | BMK_02 | 14,717,867 | 4,415,360,100 | 96.00 | 98.95 | 93.89 | 37.08 |
Daim duab 1. Kev faib ua haujlwm zoo nrog nyeem hauv txhua qhov qauv
Daim duab 2. Puag cov ntsiab lus xa tawm
Daim duab 3. Kev faib tawm ntawm cov ntawv nyeem hauv cov ntaub ntawv ua ntu zus
