Exclusive Agency for Korea

BMKCLOURT nkag mus

Khoom

DNA / RNA EXTENCING - Nanopore Sequencer

Ont externenction yog ib qho lwg me me tiag tiag ua ntu hluav taws xob ua ntu zus raws li Nanopores, lub hauv paus ntsiab lus ntawm txhua lub platform yog tib yam. Muab ob npaug rau pov tseg DNA / RNA yuav khi rau cov protein nanopnor sib txawv los ntawm ob tog ntawm biofilm, DNA / RNA strands dhau los ntawm Nanopore channel protein ntawm ib qho tus nqi. Vim yog cov sib txawv ntawm cov tshuaj lom neeg ntawm cov hauv paus sib txawv ntawm DNA / RNA strand, thaum ib lub hauv paus lossis DNA cov cim hluav taws xob, nws yuav ua rau cov teeb meem hluav taws xob sib txawv. Los ntawm kev kuaj pom thiab sib raug rau cov cim no, cov hauv paus coj tuaj yeem raug suav, thiab qhov tseeb nrhiav tau ntawm cov txheej txheem tuaj yeem ua tiav.

Tiv Tauj Peb

Cov Lus Qhia Pab

Demo tshwm sim

Cov Lus Qhia Kev Pabcuam Tshwj Xeeb Nta

Sam thiaj	Lub tsev qiv ntawv loj	Theoretical cov ntaub ntawv tawm los (ib lub cell)	Ib Leeg-Lub Hauv Paus Raug	Cov Ntawv Thov
Tus pw	8kB, 10kb, 20kb, ultralong, cdna-pc	70-90GB / Cell	85-92%	SV hu, tsib Novo, tag nrho-ntev-ntev-ntev-seq, gene annotation, nrhiav kom muaj methylation

Kev Pabcuam Zoo

● Ntau tshaj 5 xyoos kev paub ntawm pacbio exctraging platform nrog ntau txhiab tus tau kaw cov haujlwm nrog ntau hom.
● Bmkgene yog tus khub ua tus khub ntawm Oxford Nanopore, nrog dual platform RNA / DNA ntawv pov thawj.
● Muaj cov qauv tseem ceeb ntawm seallers nrog cov khoom siv ua tiav thiab txaus ua ntu zus los ntawm kev ua ntu zus.
● Raws li cov kev tshawb fawb Nanopore ntau dua 10 tsiaj thiab cog cov kev tshawb fawb Denovo tau tshaj tawm hauv cov ntawv sau ua si thoob ntiaj teb.

Qauv Yuav Tsum Tau

Qauv Hom

Pes tsawg

Ecentrating (QUbb 5)

Qho loj hnyav

Kev coj dawb huv

Lwm tus

Genomic DNA

Nyob ntawm cov ntaub ntawv xav tau

≥20G / μl

≥15μl

OD260 / 280 = 1.7-2.2;

OD260 / 230≥1.5;

Ntshiab ncov ntawm 260 nm, tsis muaj kev paug

Kev xav tau xav tau kev ntsuas los ntawm Litit thiab Litit / Nanopore ≤ 2

Tag nrho RNA

≥1.2μg

≥100μg / μl

≥15μl

OD260 / 280 = 1.7-2.5;

OD260 / 230 = 0.5-2.5; Tsis muaj kev phom sij

Rin nqi ≥7.5

Kev Pabcuam Workflow

Qauv npaj

Kev Siv Hauv Tsev Qiv Ntawv

Ze

Cov Ntaub Ntawv Kev Ntsuam Xyuas

Qhov Haujlwm Xa

Yav dhau los: Illina ua ntej-ua cov tsev qiv ntawv

Tom ntej: Tseeb-Locus Fragment Fragment Sequencing (SLAF-SEQ)

Cov Ntaub Ntawv Zoo ntawm DNA Qauv

Cov lus 1. Txheeb cais ntawm cov ntaub ntawv huv.

BMKID	nyoos nojqnum	yuag	kev ntxuav cia kom huv	huv si	ntxhua huv	ntxhua huv	kev huv nkaus	kev ntxuav kom huv	kev huv si
DNA_BMK01	1,218,239	26.37	1,121,736	25.90	28,014	15,764	23,090	143,181	9