Illina ua ntej-ua cov tsev qiv ntawv
Nta
●Platforms:Ecina novaseq 6000 thiab novaseq x plus
●Summing hom:PE150 thiab PE250
●Kev tswj hwm zoo ntawm cov tsev qiv ntawv ua ntej ua ntu zus
●Cov ntaub ntawv QC thiab kev xa khoom:Kev xa cov QC daim ntawv qhia thiab cov ntaub ntawv nyoos hauv cov hom ceev tom qab demultipleXing thiab lim Q30 nyeem
Kev Pabcuam Zoo
●Versatility ntawm seals cov kev pab:Cov qhua tuaj noj mov tuaj yeem xaiv cov kab ke los ntawm txoj kab, ntws ntawm tes, lossis los ntawm cov ntaub ntawv yuav tsum muaj (ib nrab kab kev sib txuas).
●Cov kev paub dav dav ntawm ICYE SEFRATION PLATFORM:nrog ntau txhiab tus haujlwm tau kaw nrog ntau hom.
●Kev Xa Tawm ntawm Sequencing QC qhia:Nrog kev ntsuas zoo, cov ntaub ntawv raug thiab tag nrho kev ua tau zoo ntawm kev ua ntu zus.
●Cov Txheej Txheem Elixed:nrog luv tig-ncig.
●Kev tswj kom nruj: Peb siv nruj QC cov kev xav tau kom lav tau tias kev xa cov txiaj ntsig zoo hauv cov txiaj ntsig zoo.
Qauv Platform
| Sam thiaj | Night cell | Seem Vomencing hom | Ib yam | Kwv yees kwv yees |
| Novaseq x | 10b (8 txoj kab) | PE150 | Ib txoj kab Ib nrab kab | 375gb / kab |
| 25b (8 kab) | PE150 | Ib txoj kab Ib nrab kab | 1000 GB / LANE | |
| Novaseq 6000 | Sp ntws (2 txoj kab) | Feem pua250 | Night cell Ib txoj kab Ib nrab kab | 325-400 m nyeem / txoj kab |
| S4 ntws cell (4 txoj kab) | PE150 | Night cell Ib txoj kab Ib nrab kab | ~ 800 GB / LANE |
Qauv Yuav Tsum Tau
| Cov ntaub ntawv tus nqi (x) | Concentration (QPCR / NM) | Qho loj hnyav | |
| Ib nrab kab sib txuas
| X ≤ 10 GB | ≥ 1 nm | ≥ 25 μl |
| 10 GB <X ≤ 50 GB | ≥ 2 nm | ≥ 25 μl | |
| 50 gb <X ≤ 100 GB | ≥ 3 nm | ≥ 25 μl | |
| X> 100 gb | ≥ 4 nm | ≥ 25 μl | |
| Txoj kab kev ua ntu zus | Ib txoj kab | ≥ 1.5 NM / FARDED FARDON | ≥ 25 μl / Tsev qiv Ntawv Pas |
Ntxiv rau kev xav thiab tag nrho cov nyiaj, ib qho qauv peak zoo kuj tseem xav tau.
Nco tseg: Txoj kab kev sib txuas ntawm cov tsev qiv ntawv muaj ntau haiv neeg yuav tsum phix taws-hauv kom muaj kev hu kom ruaj khov.
Peb pom zoo xa cov tsev qiv ua ntej ua ntej. Yog tias koj xav tau BMKgene los ua cov tsev qiv ntawv pas, thov saib mus
Cov tsev qiv ntawv xav tau rau txoj kab ib nrab ua ntu zus.
Lub tsev qiv ntawv loj (peak daim ntawv qhia)
Lub ntsiab tseem ceeb yuav tsum tsis pub dhau 300-450 bp.
Cov tsev qiv ntawv yuav tsum muaj ib lub ncov loj, tsis muaj kev sib kis thiab tsis muaj qhov chaw tso quav.
Thov ncav cuag peb yog tias koj cov qauv tsis tau raws li cov khoom yuav tsum tau ua.
Kev Pabcuam Workflow
Loos Living Tswjhwm
Ze
Cov Ntaub Ntawv Tswjhwm Zoo
Qhov Haujlwm Xa
Daim Ntawv Qhia QC Daim Ntawv Qhia
Daim ntawv tshaj tawm nyob rau hauv qhov zoo ntawm lub tsev qiv ntawv tau muab ua ntej ua ntu zus, ntsuas chaw qiv ntawv, thiab tawg.
Txuas QC Daim Ntawv Qhia
Cov lus 1. Txheeb cais ntawm cov ntaub ntawv sib txuas.
| Qauv ID | BMKID | RAW tau nyeem | Cov ntaub ntawv nyoos (Bp) | Cov nyeem kom huv (%) | Q20 (%) | Q30 (%) | GC (%) |
| C_01 | BMK_01 | 22,870,120 | 6,861,036,000 | 96.48 | 99.14 | 94.85 | 36.67 |
| C_02 | BMK_02 | 14,717,867 | 4,415,360,100 | 96.00 | 98.95 | 93.89 | 37.08 |
Daim duab 1. Kev faib ua haujlwm zoo nrog nyeem hauv txhua qhov qauv
Daim duab 2. Puag cov ntsiab lus xa tawm
Daim duab 3. Kev faib tawm ntawm cov ntawv nyeem hauv cov ntaub ntawv ua ntu zus
