Circrna Seem-artina
Nta
● RRNA DePetion ua raws li los ntawm cov ntaub ntawv qhia txog lub tsev qiv ntawv, ua kom cov ntaub ntawv sib txawv tshwj xeeb.
● Bioinformic ua haujlwm ua haujlwm rau Circrau twv ua ntej thiab cov lus qhia kom muaj nuj nqis
Kev Pabcuam Zoo
●Ntau tshaj cov chaw qiv ntawv RNA:Peb siv RRA depetion es tsis txhob ua linear RNA qhov kev xaiv nyob hauv peb cov ntaub ntawv npaj ua ntej, kom ua tiav thiab lncring thiab lnging kev tshuaj xyuas ntawm cov ntaub ntawv no
●Kev Tshawb Fawb Txog Kev Sib Tw Thaum Lausogenous RNA (CERNA) Tes hauj lwm: Muab cov ntaub ntawv tob tob rau hauv kev tswj hwm cellular
●Kev txawj ntse dav: Nrog cov ntaub ntawv teev tseg ntawm kev ua cov qauv 20,000 ntawm BMKGENE, ncua cov phiaj xwm muaj hom thiab LNCND Prosts, peb pab neeg coj kev muaj txiaj ntsig rau txhua qhov project.
●Kev tswj kom nruj: Peb siv cov ntsiab lus tseem ceeb thoob plaws txhua theem, los ntawm cov qauv thiab chaw khaws ntawv npaj rau kev sib txuas thiab bioinformics. Qhov kev saib xyuas me me no ua kom pom tseeb qhov kev xa tawm ntawm cov txiaj ntsig zoo.
● Kev them nyiaj yug tom qab muag: Peb cov kev cog lus txuas ntxiv tshaj qhov ua tiav nrog 3-hli tom qab muag lub sijhawm pabcuam. During this time, we offer project follow-up, troubleshooting assistance, and Q&A sessions to address any queries related to the results.
Qauv yuav tsum tau ua thiab xa khoom
| Lub tsev qiv ntawv | Sam thiaj | Cov ntaub ntawv pom zoo | Cov ntaub ntawv QC |
| RRNA Depleted Directional Lub Tsev Qiv Ntawv | ICATINA PE150 | 16-20 GB | Q30-85% |
Qauv Yuav Tsum Tau Ua:
Nucleotides:
| Conc. (NG / μl) | Tus nqi (μg) | Kev coj dawb huv | Kev muaj siab dawb paug |
| ≥ 80 | ≥ 0.8 | OD260 / 280 = 1.7-2.5 OD260 / 230 = 0.5-25-2.5 Txwv tsis pub lossis tsis muaj protein los yog cov khoom tsis huv uas muaj nyob ntawm gel. | Rin≥6.0; 5.0 ≥28s / 18S≥1.0; Txwv tsis pub lossis tsis muaj qhov nce hauv qab |
● ● Nroj tsuag:
Hauv paus, kav lossis Petal: 450 mg
Nplooj lossis noob: 300 mg
Txiv Hmab Txiv Ntoo: 1.2 g
Tsiaj:
Lub plawv lossis txoj hnyuv: 450 mg
Viscera lossis Hlwb: 240 mg
Cov leeg: 600 mg
Cov pob txha, plaub hau lossis tawv nqaij: 1.5g
Arthropods:
Kab: 9G
Crustacea: 450 mg
Ntshav tag nrho:2 leeg
● Cells: 106 ig
● Ntshav thiab ntshav: 6 ML
Pom zoo tus qauv xa mus
Thawv: 2 Ml Centrifuge Tube (Tin Ntawv Nyiaj tsis pom zoo)
Qauv kos npe: Pab pawg + hloov kho dua li AG1, A2, A3; B1, B2, B3.
Cov zaub mov xa tuaj:
1. Cov dej khov txias: Cov qauv yuav tsum tau ntim rau hauv cov hnab thiab faus rau hauv cov dej khov qhuav.
2. Rnastable Hlis: RNA cov qauv tuaj yeem qhuav hauv RNA stabilization raj (piv txwv li, rnastable®) thiab xa tawm ntawm chav tsev kub.
Kev Pabcuam Khiav
Kev Tshawb Fawb Kev Tshawb Fawb
Qauv Xa
RNA rho tawm
Kev Siv Hauv Tsev Qiv Ntawv
Ze
Cov Ntaub Ntawv Kev Ntsuam Xyuas
Cov Kev Pab Tom Qab Muag
Bioinformformic
Circrna Kev twv ua ntej: chromosomal faib
Kev Tshaj Tawm Tshaj Tawm Circrnas - Roob hluav taws
Sib txawv hais txog Circrnas - Hierarchical Clustering
Ua haujlwm rau Circraw tus tswv cuab cov noob
Tshawb cov kev tshawb fawb txog kev tshawb fawb los ntawm BMKGENE 'CIRCRAN EXTRECTION cov kev pabcuam los ntawm kev sau cov ntawv sau los ntawm kev sau cov ntawv tshaj tawm.
Wang, X. li al. (2021) 'CPSF4 tswj Circra tsim thiab Micrigna cov khoom siv kho kom haum rau cov roj av hauv Hepatocellular Carcinoma', oncogene carcinoma ', oncogene 2021 40:25-43338-4351. DOI: 10.18 / s41388-021-01867-6.
Xia, K. li al. (2023) 'X oo-teb kev qhia tawm lub luag haujlwm ntawm OSARAB hauv Roo133 Kev Tshawb Fawb Txog Kev Tshawb Fawb, 5 (1), pp. 1-14. DOI: 10.1186 / s42483-023-00188-8 / Nuj / Nuj nqis / 6.
Y, H. li al. (2023) 'CPSF3 modulates qhov sib npaug ntawm cov voj voog thiab linear cov ntawv sau hauv Hepatocellular Carcinoma'. DOI: 10.21203 / Rs.3.rs-2418311 / v1.
Zhang, Y. li al. (2023) 'Kev Ntsuam Xyuas Cov Circrnas hauv Cirrhotic Cardiomenyopathy Ua ntej thiab tom qab lub siab hloov impplantation', International Kev Khoos Phis Nyuaj ', 114, p. 109495. DOI: 10.1016 / J.Inimp.20222229495.
