BMKMANU S3000_SPATIAial transcriptome
BMKManu S3000 spatial transcriptome txuj ci txheej txheem
Nta
- Kev daws teeb meem: 3.5 μm
- Saib txoj kab uas hla: 2.5 μm
- Tus naj npawb ntawm cov pob: kwv yees li 4 lab
- 3 Lub Zos Yuav Tsum Tau Ua: 6.8 hli * 6.8 hli, 11 hli * 11 hli lossis 15 hli * 20 hli
- Txhua Barcoded hlaws yog thauj khoom nrog cov ntawv tshaj tawm ntawm 4 ntu:
• POLY (DT) tus Tsov tus tw rau mRNA priming thiab cdna synthesis,
• Cov cim roj ntsha txhais tau tias (UMI) los kho qhov tsis ncaj ncees
• Spatial Barcode
• Kev khi ua ntu zus ntawm kev nyeem ib nrab 1 ua ntu zus
- H & E thiab fluorescent staining ntawm ntu
- Muaj peev xwm siv cov xov tooj ntawm tes ua haujlwm: kev sib xyaw ntawm H & E staining, fluorescent staining, thiab RNA txoj kev muab tshaj tawm qhia rau txhua lub cell. Ua cov txheej txheem hauv qab kev tshawb xyuas cov khoom siv tau raws li ntawm tes rau hauv.
- Muaj peev xwm ua tiav kev soj ntsuam Multilevel Daws Teeb Meem: Kev siv tau ntau-qib kev txheeb xyuas txij li 100 teev rau 3.5 um los daws cov pa hluav taws xob ntau yam.
Qhov Zoo Ntawm BMKManu S3000
-Ob npaug ntawm kev ntes me ntsis rau 4 lab: Nrog kev daws teeb meem txhim kho ntawm 3.5 um, ua rau cov noob siab dua thiab UMI nrhiav tau ib lub cell. Qhov no ua rau muaj kev txhim kho pawg ntawm cov hlwb raws li kev hloov pauv cov ntaub ntawv, nrog cov ntawv qhia kom zoo uas phim cov qauv ntaub ntawv.
- Sub-Cellular daws teeb meem:Txhua thaj chaw ntes tau muaj> 2 lab spatial barcoded me ntsis nrog ib lub cheeb ntawm 2.5 μm thiab qhov chaw sib nrug, ua kom muaj spatial transcriptome nrog sub-cellular daws teeb meem (5 μm).
-Ntau qib kev soj ntsuam kev daws teeb meem:Kev siv hluav taws xob ntau ntau los ntawm 100 μm txog 5 μm los daws cov ntsej muag sib txawv ntawm kev daws teeb meem zoo tshaj plaws.
-Muaj peev xwm siv "peb nyob rau hauv ib qho swb" ntawm tes segmentation thev naus laus zis:Sib txuas fluorescence staining, h & e qhov staining, thiab RNA excuring ntawm ib lub vias ntawm ib thaj tsam ntawm tes rau cov transcriptomics tom qab.
-Tau tshaj ntau yam sib npaug: Ob qho tib si NGS thiab ntev-nyeem cov txheej txheem muaj.
-Saj zawg zog tsim ntawm 1-8 Lub chaw ua haujlwm nquag: Qhov luaj li cas ntawm thaj chaw ntes yog hloov tau, yog muaj peev xwm siv 3 hom (6.8 hli * 6.8 hli * 11 hli * 20 hli)
-Kev Pabcuam One-Nres: Ua ke txhua yam kev paub thiab kev kawm ua cov txheej txheem, suav nrog Cryo-socking, special barcoding, chaw npaj, ua ntu zus, thiab bioinformatics, thiab bioinformics.
-Kev ua kom zoo rau Bioinformformics thiab cov neeg siv kev pom zoo ntawm cov txiaj ntsig:Pob suav nrog 29 kev tshuaj xyuas thiab 100+ cov nuj nqis zoo, sib xyaw nrog kev siv software kom pom thiab cov chaw cell sib xws thiab cov chaw ntawm tes.
-Customized cov ntaub ntawv tsom xam thiab kev pom: muaj rau kev tshawb fawb sib txawv
-Cov txuj ci siab tshaj lij pab pawg: Nrog kev paub dhau 250 cov nqaij hom thiab 100+ hom suav nrog rau tib neeg, nas, tsiaj, ntses thiab nroj tsuag.
-Real-time hloov tshiab ntawm tag nrho cov phiaj xwm: Nrog kev tswj xyuas tag nrho ntawm kev sim ua kev sim.
-Kev xaiv sib koom tes kev txheeb xyuas nrog ib leeg ntawm Cell MRNA Exportensing
Kev Pabcuam Tshwj Xeeb
| Qauv Yuav Tsum Tau | Lub tsev qiv ntawv | Seitencing Lub Tswv Yim | Cov ntaub ntawv pom zoo | Kev Tswj Xyuas Zoo |
| Os-embedded Cryo Cov Qauv (Kev ua siab ntau tshaj: kwv yees. 6 × 6 × 6 mm ... 2 blocks ib qho piv txwv 1 rau kev sim, 1 rau thaub qab | S3000 CDNA Qiv Ntawv | ICATINA PE150 | 160K PE Nyeem txog 100υM (250 GB) | Rin> 7 |
Yog xav paub ntxiv txog cov qauv kev npaj thiab kev pabcuam workflow, thov koj tham nrog aBMKGENE CART
Kev Pabcuam Khiav
Hauv cov qauv npaj theem, ib qho pib RNA extraction sim yog ua kom pom zoo RNA tuaj yeem tau txais. Nyob rau hauv daim ntaub ua kom nce qib ntu yog stained thiab pom thiab permeagilization tej xwm txheej rau mrna tso tawm ntawm cov nqaij yog qhov zoo. Tom qab cov qauv zoo li qub tau thov thaum lub tsev qiv ntawv siv, ua raws kev ua ntu zus thiab cov ntaub ntawv tsom xam.
Qhov kev pabcuam ua tiav ua haujlwm koom nrog lub sijhawm tshiab thiab cov neeg siv tau lees paub los tswj cov vaj lug kub ua kom tiav, ua kom tiav txoj haujlwm du.
Cov ntaub ntawv tsim los ntawm BMKManu S3000 yog tshuaj xyuas siv software "BSTMATRIX", uas yog tsim kev daws teeb meem ntawm tes thiab kev daws teeb meem ntawm tes thiab cov khoom siv daws teeb meem. Los ntawm muaj, tus qauv qhia yog tsim tawm uas suav nrog cov ntaub ntawv zoo tswj, cov qauv kev soj ntsuam sab hauv thiab pab pawg txheeb xyuas.
- Cov ntaub ntawv zoo tswj:
- Cov ntaub ntawv tawm thiab cov qhab nia zoo
- noob nrhiav tau ib qho chaw
- Cov nqaij npog
- Cov qauv sab hauv:
- Kev nplua nuj
- Cov chaw sawv, suav nrog txo qis dogension
- Cov lus qhia kev sib txawv ntawm pawg: ID cim ntawm tus cim cim
- Kev ua haujlwm tshaj tawm thiab cov khoom siv ntawm cov cim cim
- Kev Ntsuam Xyuas Kev Ntsuas
- Rov ua ke ntawm cov pob me me los ntawm ob qho qauv (piv txwv li muaj mob thiab tswj) thiab rov ua dua tshiab
- Kev txheeb xyuas cov cim cim rau txhua pawg
- Kev ua haujlwm tshaj tawm thiab cov khoom siv ntawm cov cim cim
- Cov lus sib txawv ntawm tib pawg ntawm pawg
Ib qho ntxiv, BMKGAE tsim "BSTVIEY" yog cov neeg siv-tus neeg siv khoom siv rau kev nthuav qhia cov caj ces thiab cov nqaij sib txawv ntawm cov kev daws teeb meem sib txawv.
BMKGENE muaj cov kev pab cuam Profilial profiling ntawm kev daws teeb meem ntawm tes (raws li ntawm tes rau hauv lossis cov multilevel square rau hauv 100 teev rau 3.5 teev).
Cov ntaub ntawv hais txog kev sib txawv ntawm cov ntaub so ntswg ntawm S3000 Swb ua tau zoo li hauv qab no.
Cov Ntaub Ntawv Kev Kawm 1: Mes Lub Hlwb
Kev tsom xam ntawm ib qho nas lub hlwb lub hlwb nrog S3000 tshwm sim hauv kev txheeb xyuas ntawm ~ 44 000 cells, nrog ib lub hlwb ua ntu zus ntawm ~ 2000 gores ib cell. Kev txhim kho cov kev daws teeb meem ntawm 3.5 UM ua rau muaj cov ncauj lus ntxaws ntxaws ntawm cov hlwb raws li cov qauv khoom siv hluav taws xob, nrog cov pawg ntawm cov qauv hlwb ua ke. Qhov no tau qhia tau yooj yim los ntawm kev pom kev faib cov cells clustered li orsigodendrocytes, uas yog yuav luag tshwj xeeb thiab dawb teeb meem, feem.
Kev Kawm Kev Kawm 2: MOOS
Kev tsom xam ntawm ib tug nas embryo seem nrog S3000 ua rau cov cim ntawm ~ 2200 000 cells, nrog ib lub hlwb ua ntu zus ntawm ~ 1600 noob ib lub cell. Kev txhim kho cov kev daws teeb meem ntawm 3.5 UM ua rau muaj cov ncauj lus kom ntxaws ntxaws ntawm cov kab mob hlwb raws li cov qauv ntawm cov qauv, nrog 12 pawg nyob hauv thaj chaw ntawm lub hlwb.
Sab hauv-qauv tshuaj ntsuam ntawm tes pawg:
Tus cim cim cov cim npe thiab spatial faib:
- Ntau dua ntawm kev daws teeb meem ntawm tes: piv nrog S1000 Swb, txhua qhov chaw ntawm thaj tsam ntawm 2.5 μm thiab ib qho chaw deb ntawm kev ntsuas ntawm Cellular daws teeb meem (cov xwm txheej bin: 3.5 μm).
- Kev ntes siab dua: piv nrog S1000 SMIDE, nruab nrab, nruab nrab ntxiv txij li 30% mus rau 70%, median_gene nce los ntawm 30% mus rau 60%
Cov txheej txheem ntawm S1000 nti:
Cov txheej txheem ntawm S3000 nti:
